Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

DHX9

Uniprot No.

Q08211

Research Area

Epigenetics and Nuclear Signaling

Alternative Names

ATP dependent RNA helicase A; ATP-dependent RNA helicase A; DDX 9; DDX9; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 9; DEAD/H box 9; DEAD/H box polypeptide 9; DEAH (Asp Glu Ala His) box polypeptide 9; DEAH box polypeptide 9; DEAH box protein 9; DHX 9; dhx9; DHX9_HUMAN; Leukophysin; LKP; NDH 2; NDH II; NDH2; NDHII; Nuclear DNA helicase II; RHA

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

325-840aa

Target Protein Sequence

NQVGVVPWSPPQSNWNPWTSSNIDEGPLAFATPEQISMDLKNELMYQLEQDHDLQAILQERELLPVKKFESEILEAISQNSVVIIRGATGCGKTTQVPQFILDDFIQNDRAAECNIVVTQPRRISAVSVAERVAFERGEEPGKSCGYSVRFESILPRPHASIMFCTVGVLLRKLEAGIRGISHVIVDEIHERDINTDFLLVVLRDVVQAYPEVRIVLMSATIDTSMFCEYFFNCPIIEVYGRTYPVQEYFLEDCIQMTHFVPPPKDKKKKDKDDDGGEDDDANCNLICGDEYGPETRLSMSQLNEKETPFELIEALLKYIETLNVPGAVLVFLPGWNLIYTMQKHLEMNPHFGSHRYQILPLHSQIPREEQRKVFDPVPVGVTKVILSTNIAETSITINDVVYVIDSCKQKVKLFTAHNNMTNYATVWASKTNLEQRKGRAGRVRPGFCFHLCSRARFERLETHMTPEMFRTPLHEIALSIKLLRLGGIGQFLAKAIEPPPLDAVIEAEHTLRELD
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

74.6kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Human DHX9 was expressed with the amino acid range of 325-840. This DHX9 protein is expected to have a theoretical molecular weight of 74.6 kDa. Expression of this DHX9 protein is conducted in e.coli. Fusion of the N-terminal 6xHis-SUMO tag into the DHX9 encoding gene fragment was conducted, allowing for easier detection and purification of the DHX9 protein in subsequent stages.

Human ATP-dependent RNA helicase A (DHX9) is a crucial enzyme involved in RNA metabolism. Functioning as an RNA helicase, DHX9 unwinds RNA duplexes, facilitating processes such as transcription, translation, and RNA splicing. It is vital for RNA-protein interactions and contributes to the regulation of gene expression. DHX9 is implicated in various cellular functions, including RNA virus replication and DNA repair. In molecular biology and cell biology, DHX9 is a key focus for understanding its multifaceted roles in nucleic acid processes, offering insights into RNA-related pathways and potential therapeutic applications.

Target Background

Function

Multifunctional ATP-dependent nucleic acid helicase that unwinds DNA and RNA in a 3' to 5' direction and that plays important roles in many processes, such as DNA replication, transcriptional activation, post-transcriptional RNA regulation, mRNA translation and RNA-mediated gene silencing. Requires a 3'-single-stranded tail as entry site for acid nuclei unwinding activities as well as the binding and hydrolyzing of any of the four ribo- or deoxyribo-nucleotide triphosphates (NTPs). Unwinds numerous nucleic acid substrates such as double-stranded (ds) DNA and RNA, DNA:RNA hybrids, DNA and RNA forks composed of either partially complementary DNA duplexes or DNA:RNA hybrids, respectively, and also DNA and RNA displacement loops (D- and R-loops), triplex-helical DNA (H-DNA) structure and DNA and RNA-based G-quadruplexes. Binds dsDNA, single-stranded DNA (ssDNA), dsRNA, ssRNA and poly(A)-containing RNA. Binds also to circular dsDNA or dsRNA of either linear and/or circular forms and stimulates the relaxation of supercoiled DNAs catalyzed by topoisomerase TOP2A. Plays a role in DNA replication at origins of replication and cell cycle progression. Plays a role as a transcriptional coactivator acting as a bridging factor between polymerase II holoenzyme and transcription factors or cofactors, such as BRCA1, CREBBP, RELA and SMN1. Binds to the CDKN2A promoter. Plays several roles in post-transcriptional regulation of gene expression. In cooperation with NUP98, promotes pre-mRNA alternative splicing activities of a subset of genes. As component of a large PER complex, is involved in the negative regulation of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms. Acts also as a nuclear resolvase that is able to bind and neutralize harmful massive secondary double-stranded RNA structures formed by inverted-repeat Alu retrotransposon elements that are inserted and transcribed as parts of genes during the process of gene transposition. Involved in the positive regulation of nuclear export of constitutive transport element (CTE)-containing unspliced mRNA. Component of the coding region determinant (CRD)-mediated complex that promotes cytoplasmic MYC mRNA stability. Plays a role in mRNA translation. Positively regulates translation of selected mRNAs through its binding to post-transcriptional control element (PCE) in the 5'-untranslated region (UTR). Involved with LARP6 in the translation stimulation of type I collagen mRNAs for CO1A1 and CO1A2 through binding of a specific stem-loop structure in their 5'-UTRs. Stimulates LIN28A-dependent mRNA translation probably by facilitating ribonucleoprotein remodeling during the process of translation. Plays also a role as a small interfering (siRNA)-loading factor involved in the RNA-induced silencing complex (RISC) loading complex (RLC) assembly, and hence functions in the RISC-mediated gene silencing process. Binds preferentially to short double-stranded RNA, such as those produced during rotavirus intestinal infection. This interaction may mediate NLRP9 inflammasome activation and trigger inflammatory response, including IL18 release and pyroptosis. Finally, mediates the attachment of heterogeneous nuclear ribonucleoproteins (hnRNPs) to actin filaments in the nucleus.; (Microbial infection) Plays a role in HIV-1 replication and virion infectivity. Enhances HIV-1 transcription by facilitating the binding of RNA polymerase II holoenzyme to the proviral DNA. Binds (via DRBM domain 2) to the HIV-1 TAR RNA and stimulates HIV-1 transcription of transactivation response element (TAR)-containing mRNAs. Involved also in HIV-1 mRNA splicing and transport. Positively regulates HIV-1 gag mRNA translation, through its binding to post-transcriptional control element (PCE) in the 5'-untranslated region (UTR). Binds (via DRBM domains) to a HIV-1 double-stranded RNA region of the primer binding site (PBS)-segment of the 5'-UTR, and hence stimulates DHX9 incorporation into virions and virion infectivity. Plays also a role as a cytosolic viral MyD88-dependent DNA and RNA sensors in plasmacytoid dendritic cells (pDCs), and hence induce antiviral innate immune responses. Binds (via the OB-fold region) to viral single-stranded DNA unmethylated C-phosphate-G (CpG) oligonucleotide.

Gene References into Functions

our study supports the presence of a p53-independent mechanism of cell death and cell cycle arrest resulting from DHX9 inhibition. our results support the feasibility of targeting DHX9 as a chemotherapeutic approach in p53-deficient tumors. PMID: 28427210
study identified the RNA helicase DHX9 as a regulator of pRNA processing; DHX9 binds to rRNA genes only upon embryonic stem cells differentiation and its activity guides TIP5 to rRNA genes and establishes heterochromatin PMID: 28588071
the decreased growth of osteosarcoma cells by MCM2 or MCM3 knockdown was reversed by DHX9 overexpression, indicating that MCM2 and MCM3 activity was DHX9-dependent. PMID: 28460433
analysis of the structure, biochemistry, and biology of DHX9, its role in cancer and other human diseases [review] PMID: 27034008
RNA helicase A was shown to cooperate with both tumor suppressors and oncoproteins in different tumours, indicating that its specific role in cancer is strongly influenced by the cellular context. PMID: 26885691
Importantly, binding of Nup98 to DHX9 stimulates the ATPase activity of DHX9, and a transcriptional reporter assay suggests Nup98 supports DHX9-stimulated transcription. PMID: 28221134
Our results demonstrate a robust tolerance for systemic DHX9 suppression in vivo and support the targeting of DHX9 as an effective and specific chemotherapeutic approach. PMID: 26973242
an evolutionarily conserved function of DHX9, it acts as a nuclear RNA resolvase that neutralizes the immediate threat posed by Alu transposon insertions and allows these elements to evolve as tools for the post-transcriptional regulation of gene expression PMID: 28355180
study provides evidence demonstrating that the PRRSV nucleocapsid protein interacts with Nsp9 and its RNA-dependent RNA-polymerase domain and also recruits the cellular helicase DHX9 during virus infection to facilitate viral RNA synthesis and virus production PMID: 27009951
Genotoxic stress inhibits Ewing sarcoma cell growth by modulating alternative pre-mRNA processing of the RNA helicase DHX9. PMID: 26450900
OB-fold is involved in modulating HIV-1 RNA splicing in the context of some HIV-1 strains while it is dispensable for the activation of HIV-1 transcription. PMID: 25149208
results demonstrate an essential role of DHX9 in DNA replication and normal cell cycle progression. PMID: 24990949
Results show RNA helicase DHX9 as an interacting partner of KIF1Bbeta and found that DHX9 is necessary for KIF1Bbeta to induce apoptosis. PMID: 24469107
The conserved lysine residues of dsRBDs play critical roles in the promotion of HIV-1 production by RNA helicase A. PMID: 24726449
RNA helicase A participates in HIV-1 RNA metabolism by multiple distinct mechanisms. PMID: 24223160
Data suggest that M029 (an RNA-binding protein) plays pivotal role in determining cellular tropism of Myxoma virus in all mammalian cells tested; human RNA helicase A/DHX9 and protein kinase R/EIF2AK2 are two binding partners of M029. PMID: 23853588
The data implicate DHX9 in processing intra-molecular triplex H-DNA structures in vivo and support its role in the overall maintenance of genomic stability at sites of alternatively structured DNA. PMID: 24049074
The crystal structure of huma DHX9 RNA binding domains in complex with double stranded RNA. PMID: 23361462
L1TD1 is part of the L1TD1-RHA-LIN28 complex that could influence levels of OCT4, suggesting that L1TD1 has an important role in the regulation of stemness. PMID: 22162396
RNA helicase A (RHA) is tethered to the 5' SL of collagen mRNAs by interaction with the C-terminal domain of LARP6. PMID: 22190748
RNA helicase A as a cellular factor that interacts with influenza A virus NS1 protein and its role in the virus life cycle. PMID: 22171255
DHX9 is an important RNA sensor that is dependent on interferon-beta promoter stimulator (IPS)-1 to sense pathogenic RNA. PMID: 21957149
DHX9 also unwound RNA-based G-quadruplexes that have been reported to occur in human transcripts PMID: 21561811
the molecular basis for the activation of translation by RHA is recognition of target mRNA by the N-terminal domain that tethers the ATP-dependent helicase for rearrangement of the complex 5'-UTR. PMID: 21123178
observed a positive correlation of the nuclear expression of EGF receptor, RHA, and cyclin D1 in human breast cancer samples PMID: 20802156
DHX9 displaced the third strand from a specific triplex DNA structure and catalyzed the unwinding with a 3' --> 5' polarity with respect to the displaced third strand. PMID: 20669935
DHX9/DHX36 represent the MyD88-dependent DNA sensors in the cytosol of plasmacytoid dendritic cells and suggest a much broader role for DHX helicases in viral sensing PMID: 20696886
the preferential unwinding of RNA-containing substrates by WRN helicase is stimulated by DHX9 in vitro, both on Okazaki fragment-like hybrids and on RNA-containing 'chicken-foot' structures. PMID: 20385589
DHX9 lacks base-selective contacts, forms an unspecific but important stacking interaction with the base of the bound nucleotide and the protein can hydrolyze ATP, guanosine 5'-triphosphate, cytidine 5'-triphosphate, and uridine 5'-triphosphate. PMID: 20510246
Proteomic analysis indicates that cellular proteins interacted with Kaposi's sarcoma-associated herpesvirus viral protein kinase (ORF36), and co-immunoprecipitation reactions further reveal interactions with human RNA helicase A. PMID: 20437153
Four genes encoding BRCA1-interacting proteins were analyzed in a cohort of 96 breast cancer individuals from high-risk non-BRCA1/BRCA2 French Canadian families. PMID: 19197335
Nuclear DNA helicase II recruitment to PML nuclear bodies(NBs) is connected with transcriptional regulation of interferon-alpha-inducible genes attached to PML NBs. PMID: 12163469
propose that NDH II operates in both nucleoplasmic and nucleolar mode, and that its redistribution reflects accumulations indicating a possible cycling of NDH II between nucleoplasm and the nucleolus PMID: 12243751
Overexpression of a truncated RHA peptide that binds to the BRCA1 carboxy-terminus prevents normal BRCA1 function & association with nuclear foci following DNA damage. It induces pleomorphic nuclei, aberrant mitoses with extra centrosomes, & tetraploidy. PMID: 12592385
DNA-PK phosphorylated recombinant NDH II in the presence of RNA PMID: 14704337
RNA helicase A in the MEF1 transcription factor complex up-regulates the MDR1 gene in multidrug-resistant cancer cells PMID: 14769796
RHA interacts with NF-kappa B p65 and functions as a transcriptional coactivator. PMID: 15355351
Results suggest that nuclear DNA helicase II plays a role in promoting the DNA processing function of Werner syndrome helicase, which in turn might be necessary for maintaining genomic stability. PMID: 15995249
Our results indicate that the nuclear import of RHA is mediated by the importin-alpha3/importin-beta-dependent pathway and suggest that the specificity for importin may regulate the functions of cargo proteins. PMID: 16375861
RHA represents the first example of cellular RNA helicases that participate in HIV-1 particle production and promote viral reverse transcription PMID: 16527808
Phosphorylated Zic2 protein makes a stable complex with RNA helicase A. PMID: 17251188
RNA interference is a conserved pathway of sequence-specific gene silencing that depends on small guide RNAs and the action of proteins assembled in the RNA-induced silencing complex (RISC). PMID: 17531811
In vitro dephosphorylation of HeLa cellular HDH II/Ku caused a significant decrease in the DNA helicase activity of this enzyme. PMID: 18053790
These results establish a link between the p53 tumor suppressor and RNA processing via hnRNPA2/B1 and RNA Helicase A. PMID: 18519039
knockdown of DHX-9 by siRNA did not inhibit the rRNA synthesis or cause the nucleolar disruption PMID: 18600529
A 21-day follow-up of the response of HCV replication to the presence and absence of RNAi indicated that RHA is a cellular factor involved in the HCV replication process. PMID: 18782589
protein kinase R (PKR) is shown to interact with RNA helicase A; RHA is identified as a substrate for PKR, with phosphorylation perturbing the association of the helicase with double-stranded RNA PMID: 19229320
Results show overexpression or depletion of RHA could influence the interaction of Pol II with beta-actin and beta-actin-involved gene transcription regulation. PMID: 19309309

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Subcellular Location

Nucleus. Nucleus, nucleoplasm. Nucleus, nucleolus. Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.

Protein Families

DEAD box helicase family, DEAH subfamily

Database Links

HGNC: 2750

OMIM: 603115

KEGG: hsa:1660

STRING: 9606.ENSP00000356520

UniGene: Hs.191518

Code	CSB-EP600078HU
Abbreviation	Recombinant Human DHX9 protein, partial
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP600078HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) DHX9. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP600078HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) DHX9.
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Recombinant Human ATP-dependent RNA helicase A (DHX9), partial

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