Recombinant Human ATP-dependent RNA helicase DDX54 (DDX54), partial

Code CSB-YP844726HU
MSDS
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Source Yeast
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Code CSB-EP844726HU
MSDS
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Source E.coli
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Code CSB-EP844726HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP844726HU
MSDS
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Source Baculovirus
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Code CSB-MP844726HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DDX54
Uniprot No.
Alternative Names
2410015A15Rik; AI414901; ATP dependent RNA helicase DDX54; ATP-dependent RNA helicase DDX54; ATP-dependent RNA helicase DP97; DDX54; DDX54_HUMAN; DEAD (Asp-Glu-Ala-Asp) box polypeptide 54; DEAD box helicase 54; DEAD box protein 54; DEAD box RNA helicase 97 kDa; DP97; MGC2835; RGD1562539
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Has RNA-dependent ATPase activity. Represses the transcriptional activity of nuclear receptors.
Gene References into Functions
  1. Ddx54 plays an important role in central nervous system myelination, presumably in myelin sheath formation after the differentiation of oligodendrocytes. PMID: 23239230
  2. DP97 was found to be a gene (or promoter)-selective co-activator for hCAR PMID: 22910411
  3. Identifies DEAD (Asp-Glu-Ala-Asp) box polypeptide 54 (locus 79039) as a novel nucleolar protein in HeLa cells. PMID: 11790298
  4. Identifies dead box polypeptide 54 as a 14-3-3-binding protein. PMID: 16260042
  5. Identifies DEAD box polypeptide 54 (similar to RIKEN cDNA 2410015A15 gene) as one of 213 different nucleolar proteins in HeLa cells. PMID: 12429849
  6. Kinetic analysis of DEAD box helicases, including DEAD box polypeptide 54, in response to transcription inhibition. PMID: 15635413
  7. Includes the identification of DEAD box polypeptide 54 phosphopeptides following the stimulation of HeLa cells with epidermal growth factor. PMID: 17081983

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Subcellular Location
Nucleus, nucleolus.
Protein Families
DEAD box helicase family, DDX54/DBP10 subfamily
Tissue Specificity
Expressed in the fallopian tube, cervix and uterus. Also expressed in the brain.
Database Links

HGNC: 20084

OMIM: 611665

KEGG: hsa:79039

STRING: 9606.ENSP00000323858

UniGene: Hs.506861

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