Recombinant Human Actin filament-associated protein 1-like 1 (AFAP1L1)

Code CSB-YP851559HU
MSDS
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Source Yeast
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Code CSB-EP851559HU
MSDS
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Source E.coli
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Code CSB-EP851559HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP851559HU
MSDS
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Source Baculovirus
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Code CSB-MP851559HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
AFAP1L1
Uniprot No.
Alternative Names
Actin filament-associated protein 1-like 1; AF1L1_HUMAN; AFAP1-like protein 1; Afap1l1; AI173486; RGD1311580
Species
Homo sapiens (Human)
Expression Region
1-768
Target Protein Sequence
MDRGQVLEQL LPELTGLLSL LDHEYLSDTT LEKKMAVASI LQSLQPLPAK EVSYLYVNTA DLHSGPSFVE SLFEEFDCDL SDLRDMPEDD GEPSKGASPE LAKSPRLRNA ADLPPPLPNK PPPEDYYEEA LPLGPGKSPE YISSHNGCSP SHSIVDGYYE DADSSYPATR VNGELKSSYN DSDAMSSSYE SYDEEEEEGK SPQPRHQWPS EEASMHLVRE CRICAFLLRK KRFGQWAKQL TVIREDQLLC YKSSKDRQPH LRLALDTCSI IYVPKDSRHK RHELRFTQGA TEVLVLALQS REQAEEWLKV IREVSKPVGG AEGVEVPRSP VLLCKLDLDK RLSQEKQTSD SDSVGVGDNC STLGRRETCD HGKGKKSSLA ELKGSMSRAA GRKITRIIGF SKKKTLADDL QTSSTEEEVP CCGYLNVLVN QGWKERWCRL KCNTLYFHKD HMDLRTHVNA IALQGCEVAP GFGPRHPFAF RILRNRQEVA ILEASCSEDM GRWLGLLLVE MGSRVTPEAL HYDYVDVETL TSIVSAGRNS FLYARSCQNQ WPEPRVYDDV PYEKMQDEEP ERPTGAQVKR HASSCSEKSH RVDPQVKVKR HASSANQYKY GKNRAEEDAR RYLVEKEKLE KEKETIRTEL IALRQEKREL KEAIRSSPGA KLKALEEAVA TLEAQCRAKE ERRIDLELKL VAVKERLQQS LAGGPALGLS VSSKPKSGET ANKPQNSVPE QPLPVNCVSE LRKRSPSIVA SNQGRVLQKA KEWEMKKT
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
May be involved in podosome and invadosome formation.
Gene References into Functions
  1. AFAP1L1 promotes cell proliferation, accelerates cell cycle progression, and prevents cell apoptosis in lung cancer cells. PMID: 29323101
  2. data define a novel pathway that directs Lyn/Src family tyrosine kinase signals to sarcoma cell invadopodia through specific recruitment of Vav2 and Nck2 to phosphorylated AFAP1L1, to control cell migration and invasion PMID: 26212012
  3. AFAP1L1 plays a role in the progression of colorectal cancer by modulating cell shape and motility and by inhibiting anoikis, presumably through interactions with vinculin-including protein complexes. PMID: 24723436
  4. Findings indicate a role for transcription factor Sp3 in sarcomas as a driver for expression of the metastasis-related gene AFAP1L1 (actin filament-associated protein 1-like 1). PMID: 23326307
  5. AFAP1L1 has a role in the progression of spindle cell sarcomas and is a prognostic biomarker. PMID: 21516130
  6. Data suggest that AFAP1L1 may forge unique protein interactions in which AFAP1 is less efficient, and these interactions may allow AFAP1L1 to affect invadosome formation. PMID: 21333378

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Subcellular Location
Cytoplasm. Cell projection, podosome. Cell projection, invadopodium. Cytoplasm, cytoskeleton, stress fiber.
Tissue Specificity
Expressed in breast, colon and brain. In all 3 tissues, expressed in the microvasculature (at protein level). In addition, in the breast, found in the contractile myoepithelial cell layer which surrounds the breast ducts (at protein level). In the colon,
Database Links

HGNC: 26714

OMIM: 614410

KEGG: hsa:134265

STRING: 9606.ENSP00000296721

UniGene: Hs.483793

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