Recombinant Human Acylpyruvase FAHD1, mitochondrial (FAHD1)

Code CSB-YP750807HU
MSDS
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Source Yeast
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Code CSB-EP750807HU
MSDS
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Source E.coli
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Code CSB-EP750807HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP750807HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP750807HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
FAHD1
Uniprot No.
Alternative Names
Acylpyruvase FAHD1; C16orf36; Chromosome 16 open reading frame 36; DKFZP566J2046; FAHD1; FAHD1_HUMAN; Fumarylacetoacetate hydrolase domain containing protein 1; Fumarylacetoacetate hydrolase domain-containing protein 1; MGC74876; mitochondrial; YISK like; YISK like/RJD15; YisK-like protein; YISKL
Species
Homo sapiens (Human)
Expression Region
28-224
Target Protein Sequence
ADH VREMRSAVLS EPVLFLKPST AYAPEGSPIL MPAYTRNLHH ELELGVVMGK RCRAVPEAAA MDYVGGYALC LDMTARDVQD ECKKKGLPWT LAKSFTASCP VSAFVPKEKI PDPHKLKLWL KVNGELRQEG ETSSMIFSIP YIISYVSKII TLEEGDIILT GTPKGVGPVK ENDEIEAGIH GLVSMTFKVE KPEY
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Probable mitochondrial acylpyruvase which is able to hydrolyze acetylpyruvate and fumarylpyruvate in vitro. Also has oxaloacetate decarboxylase activity.
Gene References into Functions
  1. The results indicate that FAHD1 is required for mitochondrial function in human cells and provide additional support to the growing evidence that mitochondrial dysfunction can induce cellular senescence by metabolic alterations independent of the DNA damage response pathway. PMID: 28286170
  2. we recently identified human FAH domain containing protein-1 (FAHD1) as the first eukaryotic oxaloacetate decarboxylase..these data establish a role of fahd-1 to maintain mitochondrial function and consequently physical activity PMID: 26266933
  3. Based on molecular modeling and subsequent biochemical investigations, the study identified FAHD1 as a eukaryotic oxaloacetate decarboxylase enzyme. PMID: 25575590
  4. identified mammalian FAHD1 as a novel mitochondrial enzyme with acylpyruvate hydrolase activity. PMID: 21878618
Subcellular Location
Mitochondrion. Cytoplasm, cytosol.
Protein Families
FAH family
Tissue Specificity
Ubiquitous (at protein level).
Database Links

HGNC: 14169

OMIM: 616320

KEGG: hsa:81889

STRING: 9606.ENSP00000372112

UniGene: Hs.513265

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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