Recombinant Human Adenylate kinase isoenzyme 5 (AK5)

Code CSB-YP897315HU
MSDS
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Source Yeast
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Code CSB-EP897315HU
MSDS
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Source E.coli
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Code CSB-EP897315HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP897315HU
MSDS
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Source Baculovirus
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Code CSB-MP897315HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
AK5
Uniprot No.
Alternative Names
Adenylate kinase 5; Adenylate kinase 6; Adenylate kinase isoenzyme 5; AK 5; AK5; AK6; ATP AMP transphosphorylase 5; ATP-AMP transphosphorylase 5; KAD5; KAD5_HUMAN; MGC33326
Species
Homo sapiens (Human)
Expression Region
1-562
Target Protein Sequence
MNTNDAKEYL ARREIPQLFE SLLNGLMCSK PEDPVEYLES CLQKVKELGG CDKVKWDTFV SQEKKTLPPL NGGQSRRSFL RNVMPENSNF PYRRYDRLPP IHQFSIESDT DLSETAELIE EYEVFDPTRP RPKIILVIGG PGSGKGTQSL KIAERYGFQY ISVGELLRKK IHSTSSNRKW SLIAKIITTG ELAPQETTIT EIKQKLMQIP DEEGIVIDGF PRDVAQALSF EDQICTPDLV VFLACANQRL KERLLKRAEQ QGRPDDNVKA TQRRLMNFKQ NAAPLVKYFQ EKGLIMTFDA DRDEDEVFYD ISMAVDNKLF PNKEAAAGSS DLDPSMILDT GEIIDTGSDY EDQGDDQLNV FGEDTMGGFM EDLRKCKIIF IIGGPGSGKG TQCEKLVEKY GFTHLSTGEL LREELASESE RSKLIRDIME RGDLVPSGIV LELLKEAMVA SLGDTRGFLI DGYPREVKQG EEFGRRIGDP QLVICMDCSA DTMTNRLLQR SRSSLPVDDT TKTIAKRLEA YYRASIPVIA YYETKTQLHK INAEGTPEDV FLQLCTAIDS IF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Active on AMP and dAMP with ATP as a donor. When GTP is used as phosphate donor, the enzyme phosphorylates AMP, CMP, and to a small extent dCMP. Also displays broad nucleoside diphosphate kinase activity.
Gene References into Functions
  1. Results indicate that AK5 expression significantly decreased in temporal lobe epilepsy: the expression of AK5 in epileptic brain tissue may play important roles in epilepsy, especially refractory epilepsy PMID: 27288770
  2. Data demonstrate that human adenylate kinase 5 has two separate functional domains and that both have enzymatic activity. PMID: 19647735
Subcellular Location
Cytoplasm.
Protein Families
Adenylate kinase family
Tissue Specificity
Brain specific.
Database Links

HGNC: 365

OMIM: 608009

KEGG: hsa:26289

STRING: 9606.ENSP00000346577

UniGene: Hs.559718

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