Recombinant Human Alcohol dehydrogenase 1B(ADH1B)

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Code CSB-EP001354HU
Size $1812 How to order?
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names ADH1B
Uniprot No. P00325
Research Area Metabolism
Alternative Names ADH beta subunit; ADH1B; ADH1B_HUMAN; ADH2; Alcohol dehydrogenase 1B; alcohol dehydrogenase 2 (class I); beta polypeptide; Alcohol dehydrogenase 2; Alcohol dehydrogenase subunit beta; Aldehyde reductase; DKFZp686C06125; OTTHUMP00000220192
Species Homo sapiens (Human)
Source E.coli
Expression Region 2-375aa
Target Protein Sequence STAGKVIKCKAAVLWEVKKPFSIEDVEVAPPKAYEVRIKMVAVGICRTDDHVVSGNLVTPLPVILGHEAAGIVESVGEGVTTVKPGDKVIPLFTPQCGKCRVCKNPESNYCLKNDLGNPRGTLQDGTRRFTCRGKPIHHFLGTSTFSQYTVVDENAVAKIDAASPLEKVCLIGCGFSTGYGSAVNVAKVTPGSTCAVFGLGGVGLSAVMGCKAAGAARIIAVDINKDKFAKAKELGATECINPQDYKKPIQEVLKEMTDGGVDFSFEVIGRLDTMMASLLCCHEACGTSVIVGVPPASQNLSINPMLLLTGRTWKGAVYGGFKSKEGIPKLVADFMAKKFSLDALITHVLPFEKINEGFDLLHSGKSIRTVLTF
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 55.7kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal 6xHis-SUMO-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time 3-7 business days
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Function
Catalyzes the NAD-dependent oxidation of all-trans-retinol and its derivatives such as all-trans-4-hydroxyretinol and may participate in retinoid metabolism. In vitro can also catalyzes the NADH-dependent reduction of all-trans-retinal and its derivatives such as all-trans-4-oxoretinal. Catalyzes in the oxidative direction with higher efficiency. Has the same affinity for all-trans-4-hydroxyretinol and all-trans-4-oxoretinal.
Gene References into Functions
  1. common variants of ADH1B and ALDH2 are independently associated with gout. Our findings likewise suggest that genotyping these variants can be useful for the evaluation of gout risk. PMID: 28566767
  2. ADH1B rs3811802 and ADH1C rs4147542 significantly modified the alcohol-colon cancer association in women. PMID: 29390059
  3. The results of the study do not support our hypothesis that ADH1B and ADH7 genotypes affect blood ethanol, and acetaldehyde concentration. PMID: 28731573
  4. ADH1B*2/*2 genotype and amount of alcohol consumption increase the risk of atrial fibrillation recurrence after catheter ablation PMID: 28975638
  5. ADH1B*3 was not significantly associated with drinking motives or drinking behaviors. However, significant interaction effects of ADH1B*3 with drinking motives on drinking behavior were found; the presence of an ADH1B*3 allele protected against greater drinking quantity among students with high social motives PMID: 28662358
  6. the mediation effects of single nucleotide polymorphisms (SNPs) in the ADH1B and ALDH2 genes on hepatocellular carcinoma, were investigated. PMID: 29600553
  7. rs17033 of ADH1B and rs671 of ALDH2 SNPs were associated with GC risk and smoking habit may further modify the effect of rs671. Conversely, rs4147536 of ADH1B might have a protective role in our study population. PMID: 29166882
  8. Serum triglycerides were positively associated with alcohol consumption in the ADH1B His/His group. PMID: 29176272
  9. ADH1B and CDH1 polymorphisms predict prognosis in male patients with non-metastatic laryngeal cancer PMID: 27689323
  10. The single nuclear polymorphism rs1229984 in ADH1B results in a gain of function and hence an increase in enzyme activity resulting in the production of excess acetaldehyde. It is found in 19-91% of East Asians but in 0-10% of other populations. PMID: 27575312
  11. Alcohol dehydrogenase 1B (ADH1B) catalyzes the oxidation of alcohol to acetaldehyde. PMID: 25828809
  12. Men carrying the ADH1B rs1229984 G>A polymorphism consumed significantly less alcohol than non-carriers. PMID: 26644136
  13. study demonstrates that ADH1B*3 may protect college students of African descent against alcohol outcomes, although only in low alcohol-facilitating environments. PMID: 28494133
  14. In Japanese alcoholic men, ADH1B*1/*1 genotype increased the risk of thrombocytopenia. PMID: 27991683
  15. In analysis using the ADH1B and ADH1C genotypes as instrumental variables for alcohol consumption, the hazards ratio for atrial fibrillation per genetic drink per day was 0.79 (0.44-1.41) among men and 0.64 (0.12-3.42) among women PMID: 27071860
  16. enhanced Alcohol elimination rate in ADH1B*2 carriers and the increased salivary Acetaldehyde levels in ALDH2*1/*2 carriers among intoxicated alcoholics provide possible mechanisms explaining how each genetic polymorphism a ff ects the risk of alcoholism and upper aerodigestive tract cancer. PMID: 27087413
  17. Our findings elucidated the crucial role of multiple genetic variations in ADH1B and ALDH2 as biomarkers of metachronous ESCC. PMID: 27038040
  18. Besides the amount and frequency of alcohol drinking, the combination of ALDH2 and ADH1B polymorphisms predicts smoking initiation. This study suggests that alcohol tolerance regulated by ALDH2 and ADH1B polymorphisms is associated with smoking initiation, and facilitates the development of targeted interventions to reduce smoking prevalence. PMID: 28212515
  19. This study showed ADH1B appears to affect phenotypic traits via both alcohol-mediated and alcohol-independent effects. PMID: 27187070
  20. ADH1B gene Arg47His variant was associated with the decreased esophageal cancer risk. PMID: 27450204
  21. Polymorphisms on ADH1B and ALDH2 had significant indirect effects on hepatocellular carcinoma risk, mediated through alcohol drinking PMID: 26827895
  22. nucleotide polymorphisms (SNPs) of alcohol dehydrogenase (ADH1B) and aldehyde dehydrogenase 2 (ALDH2) genes encoding enzymes of alcohol and reactive aldehyde metabolism, respectively, are prevalent among East Asians. PMID: 27927211
  23. Data suggest that activation of the AhR/ARNT (aryl hydrocarbon receptor/aryl hydrocarbon receptor nuclear translocator) signaling by AhR ligands (environmental carcinogens TCDD/3MC/PCB used here) represents novel mechanism for regulating the expression of ADH1B (alcohol dehydrogenase 1B) and other isoenzymes (ADH4, ADH6) in hepatocytes. PMID: 27055685
  24. No significant relationship was measured between ADH1B Arg47His and gastric cancer risk; ALDH2 Glu487Lys and CYP4502E1 polymorphisms could be risk factors for the development of gastric cancer in the Chinese population. PMID: 28002588
  25. ADH1B rs1229984 and ALDH2 rs671 polymorphisms increased the risk of Alzheimer's disease in all the genetic models. PMID: 27808372
  26. ADH1B Arg47His polymorphism, but not the ALDH2 Glu487Lys variation, may influence development of colorectal cancer in the Chinese population. PMID: 27706775
  27. No significant association was found between the ADH2 Arg47His polymorphism and risk of Parkinson's disease in the co-dominant, dominant, or recessive models. PMID: 27706693
  28. ADH1B polymorphisms influence bladder cancer risk associated with alcohol drinking. PMID: 26992901
  29. The faster metabolism of ethanol and acetaldehyde by the ADH1B*2 allele and ALDH2*1/*1 genotype and higher ketosis levels were associated with higher UA levels in alcoholics, while diabetes and the consumption of sake were negative determinants. PMID: 26542604
  30. Leukocyte counts of male Japanese alcoholics were affected by ADH1B and ALDH2 genetic variants. PMID: 26917006
  31. Initiating moderate red wine consumption at dinner among type 2 diabetes patients does not have a discernable effect on mean 24-h BP. Yet, a modest temporal BP reduction could be documented, and a more pronounced BP-lowering effect is suggested among fast ethanol metabolizers. PMID: 26232779
  32. The authors for the first time observed a significant difference in alcohol sensitivity between ADH1B*1/*2 and ADH1B*2/*2 in older men aged 65 and above. PMID: 26825972
  33. the combination of genotypes ADH2 * 2, CYP2E1 * 1 combined with genotype homozygous ALDH2 * 1 found in this study could be leading to the population to a potential risk to alcoholism. PMID: 26848198
  34. suggested that having the ADH1B*2/*2 genotype caused a positive reaction in subjects with the ALDH2*1/*1 genotype PMID: 25994345
  35. Even at early stages of malignant transformation, a significant decrease in ADH1B, ADH3, RDHL, and RALDH1 mRNA levels was observed in 82, 79, 73, and 64% of tumor specimens, respectively PMID: 27239845
  36. The presence of the ADH1B*2 allele and the active ALDH2*1/*1 genotype increased the odds ratio for a high TG level. PMID: 26284938
  37. Women drinking less alcohol were more likely to carry the rare allele at rs1229984, particularly for consumption before and during pregnancy. PMID: 24065783
  38. modulates alcohol consumption [meta-analysis] PMID: 26036284
  39. ADH1B polymorphisms may be related to comorbid bipolar II disorder and alcohol dependence. PMID: 26033520
  40. ADH1B and ADH1C genetic variations are not related to the development of alcoholism or susceptibility to alcoholic cirrhosis. PMID: 26042511
  41. Results show that mutation of this protein confers significant protection against alcohol dependence. PMID: 24735490
  42. ADH1B and (ALDH2) polymorphisms in Japanese employees were associated with increased risk for anxiety and depression and alcohol-related disorders. PMID: 25543168
  43. a central role for ADH1B in obesity and insulin resistance PMID: 25830378
  44. The study explores the association of ADH1B and ADH1C genotype with alcohol consumption and biomarkers of liver function. PMID: 25503943
  45. Findings indicate that ALDH2*2, rather than ADH1B2*2, is a causal variant allele for the accumulation of blood acetaldehyde and the resultant facial flushing during low alcohol consumption. PMID: 25365528
  46. Results show that different types of both divergent and stabilizing selection processes on ADH1B genotypes were observed in different parts of Asia, but all the data demonstrated a clear positive selection for allele 48His at this locus. PMID: 25019189
  47. ADH1B +3170A>G and ADH1C +13044A>G SNPs are associated with an increased risk of head and neck squamous cell carcinoma , and they could be used as biomarkers for the high-risk group of HNSCC in Koreans. PMID: 25874489
  48. Studies have shown that A carriers of ADH1B rs1229984 are less likely to drink alcohol. PMID: 24716453
  49. High expression level of ADH1B is associated with increased incidence of residual disease in high-grade serous ovarian cancer after surgery. PMID: 24756370
  50. ADH1B variants and childhood diversity were associated with alcohol related disorders in African-Americans and Whites in both men and women. PMID: 25410943

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Subcellular Location Cytoplasm.
Protein Families Zinc-containing alcohol dehydrogenase family
Database Links

HGNC: 250

OMIM: 103720

KEGG: hsa:125

STRING: 9606.ENSP00000306606

UniGene: Hs.4

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