Recombinant Human All-trans-retinol 13,14-reductase (RETSAT)

Code CSB-YP761234HU
MSDS
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Source Yeast
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Code CSB-EP761234HU
MSDS
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Source E.coli
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Code CSB-EP761234HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP761234HU
MSDS
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Source Baculovirus
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Code CSB-MP761234HU
MSDS
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Source Mammalian cell
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Product Details

Purity
≥85% (SDS-PAGE)
Target Names
RETSAT
Uniprot No.
Alternative Names
RETSAT; PPSIG; UNQ439/PRO872; All-trans-retinol 13,14-reductase; EC 1.3.99.23; All-trans-13,14-dihydroretinol saturase; RetSat; PPAR-alpha-regulated and starvation-induced gene protein
Species
Homo sapiens (Human)
Expression Region
19-610
Target Protein Sequence
KV YLGLFSGSSP NPFSEDVKRP PAPLVTDKEA RKKVLKQAFS ANQVPEKLDV VVIGSGFGGL AAAAILAKAG KRVLVLEQHT KAGGCCHTFG KNGLEFDTGI HYIGRMEEGS IGRFILDQIT EGQLDWAPLS SPFDIMVLEG PNGRKEYPMY SGEKAYIQGL KEKFPQEEAI IDKYIKLVKV VSSGAPHAIL LKFLPLPVVQ LLDRCGLLTR FSPFLQASTQ SLAEVLQQLG ASSELQAVLS YIFPTYGVTP NHSAFSMHAL LVNHYMKGGF YPRGGSSEIA FHTIPVIQRA GGAVLTKATV QSVLLDSAGK ACGVSVKKGH ELVNIYCPIV VSNAGLFNTY EHLLPGNARC LPGVKQQLGT VRPGLGMTSV FICLRGTKED LHLPSTNYYV YYDTDMDQAM ERYVSMPREE AAEHIPLLFF AFPSAKDPTW EDRFPGRSTM IMLIPTAYEW FEEWQAELKG KRGSDYETFK NSFVEASMSV VLKLFPQLEG KVESVTAGSP LTNQFYLAAP RGACYGADHD LGRLHPCVMA SLRAQSPIPN LYLTGQDIFT CGLVGALQGA LLCSSAILKR NLYSDLKNLD SRIRAQKKKN
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the saturation of all-trans-retinol to all-trans-13,14-dihydroretinol. Does not exhibit any activity toward all-trans-retinoic acid, nor 9-cis, 11-cis or 13-cis-retinol isomers. May play a role in the metabolism of vitamin A. Independently of retinol conversion, may regulate liver metabolism upstream of MLXIPL/ChREBP. May play a role in adipocyte differentiation.
Gene References into Functions
  1. RetSat plays an important role in the biology of adipocytes, where it favors normal differentiation, yet is reduced in the obese state. PMID: 19139408
Subcellular Location
Endoplasmic reticulum membrane; Peripheral membrane protein.
Protein Families
Carotenoid/retinoid oxidoreductase family, CrtISO subfamily
Tissue Specificity
Expressed in liver; expression positively correlates with obesity and liver steatosis. Expressed in adipose tissue; expression tends to be decreased in obese versus lean individuals.
Database Links

HGNC: 25991

OMIM: 617597

KEGG: hsa:54884

STRING: 9606.ENSP00000295802

UniGene: Hs.440401

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China
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