Recombinant Human Ankyrin repeat and SOCS box protein 4 (ASB4)

Code CSB-YP897287HU
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Source Yeast
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Code CSB-EP897287HU
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Source E.coli
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Code CSB-EP897287HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP897287HU
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Source Baculovirus
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Code CSB-MP897287HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ASB4
Uniprot No.
Alternative Names
Ankyrin repeat and SOCS box containing 4; Ankyrin repeat and SOCS box containing protein 4 ; Ankyrin repeat and SOCS box protein 4; ASB 4; ASB-4; ASB4; ASB4_HUMAN
Species
Homo sapiens (Human)
Expression Region
1-426
Target Protein Sequence
MDGTTAPVTK SGAAKLVKRN FLEALKSNDF GKLKAILIQR QIDVDTVFEV EDENMVLASY KQGYWLPSYK LKSSWATGLH LSVLFGHVEC LLVLLDHNAT INCRPNGKTP LHVACEMANV DCVKILCDRG AKLNCYSLSG HTALHFCTTP SSILCAKQLV WRGANVNMKT NNQDEETPLH TAAHFGLSEL VAFYVEHGAI VDSVNAHMET PLAIAAYWAL RFKEQEYSTE HHLVCRMLLD YKAEVNARDD DFKSPLHKAA WNCDHVLMHM MLEAGAEANL MDINGCAAIQ YVLKVTSVRP AAQPEICYQL LLNHGAARIY PPQFHKVIQA CHSCPKAIEV VVNAYEHIRW NTKWRRAIPD DDLEKYWDFY HSLFTVCCNS PRTLMHLSRC AIRRTLHNRC HRAIPLLSLP LSLKKYLLLE PEGIIY
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Probable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes differentiation and maturation of the vascular lineage by an oxygen-dependent mechanism.
Gene References into Functions
  1. Based on its differential expression, neuroanatomical distribution and colocalisation, we hypothesise that rat Asb-4 is a gene involved in energy homeostasis. PMID: 15929745
Protein Families
Ankyrin SOCS box (ASB) family
Database Links

HGNC: 16009

OMIM: 605761

KEGG: hsa:51666

STRING: 9606.ENSP00000321388

UniGene: Hs.602765

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