Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

APOA4

Uniprot No.

P06727

Research Area

Cardiovascular

Alternative Names

APOA4Apolipoprotein A-IV; Apo-AIV; ApoA-IV; Apolipoprotein A4

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

21-396aa

Target Protein Sequence

EVSADQVATVMWDYFSQLSNNAKEAVEHLQKSELTQQLNALFQDKLGEVNTYAGDLQKKLVPFATELHERLAKDSEKLKEEIGKELEELRARLLPHANEVSQKIGDNLRELQQRLEPYADQLRTQVNTQAEQLRRQLTPYAQRMERVLRENADSLQASLRPHADELKAKIDQNVEELKGRLTPYADEFKVKIDQTVEELRRSLAPYAQDTQEKLNHQLEGLTFQMKKNAEELKARISASAEELRQRLAPLAEDVRGNLRGNTEGLQKSLAELGGHLDQQVEEFRRRVEPYGENFNKALVQQMEQLRQKLGPHAGDVEGHLSFLEKDLRDKVNSFFSTFKEKESQDKTLSLPELEQQQEQQQEQQQEQVQMLAPLES
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

59.4kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 21-396 constitute the expression domain of recombinant Human APOA4. The expected molecular weight for the APOA4 protein is calculated to be 59.4 kDa. The APOA4 protein was expressed in e.coli. The N-terminal 6xHis-SUMO tag was fused into the coding gene segment of APOA4, making it easier to detect and purify the APOA4 recombinant protein in the later stages of expression and purification.

Apolipoprotein A-IV (APOA4) is a protein that has attracted a lot of interest in various research areas lately. In the study of lipid metabolism, researchers are mainly looking at how APOA4 impacts cholesterol and triglyceride levels. Studies show that APOA4 plays a vital role in transporting and absorbing cholesterol, crucial for maintaining a healthy balance of lipids in the body. What's especially interesting is the attention it's getting in research on metabolic and heart diseases. Its connection to issues like lipid disorders and atherosclerosis suggests it could be a useful marker and target for treatments.

Target Background

Function

May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.

Subcellular Location

Secreted.

Protein Families

Apolipoprotein A1/A4/E family

Tissue Specificity

Synthesized primarily in the intestine and secreted in plasma.

Database Links

HGNC: 602

OMIM: 107690

STRING: 9606.ENSP00000350425

UniGene: Hs.1247

Code	CSB-EP001916HU
Abbreviation	Recombinant Human APOA4 protein
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Apolipoprotein A-IV (APOA4)

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