Recombinant Human Apoptosis-stimulating of p53 protein 2(TP53BP2) ,partial

Code CSB-YP622676HU
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Source Yeast
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Code CSB-EP622676HU
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Source E.coli
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Code CSB-EP622676HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP622676HU
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Source Baculovirus
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Code CSB-MP622676HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names TP53BP2
Uniprot No. Q13625
Alternative Names 53BP2; Apoptosis stimulating of p53 protein 2; Apoptosis stimulating protein of p53 2; Apoptosis-stimulating of p53 protein 2; ASPP2; ASPP2_HUMAN; BBP; Bcl2 binding protein; Bcl2-binding protein; NY REN 51 antigen ; p53 binding protein 2; p53-binding protein 2; p53BP2; PPP1R13A; Renal carcinoma antigen NY-REN-51; Tp53bp2; Tumor protein p53 binding protein 2; Tumor suppressor p53 binding protein 2; Tumor suppressor p53-binding protein 2
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions with proteins such as TP53. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of NAE1 to conjugate NEDD8 to CUL1, and thereby decreases NAE1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42.
Gene References into Functions
  1. Study have provided evidence that ASPP2 has an inhibitory influence on triplenegative breast cancer (TNBC) growth and metastasis, and that this may rely on using the PI3K/AKT pathway in a p53independent manner. PMID: 29568874
  2. the abnormal expression of ASPP2 may play an important role for development and metastasis in esophageal squamous cell carcinoma. PMID: 29747775
  3. These findings identify TP53BP2 as a strong candidate causative gene for central nervous system (CNS) defects in 1q41q42 microdeletion syndrome. PMID: 27447114
  4. Results showed that protein expression levels of ASPP2 and P53 were significantly higher in esophageal squamous cell carcinoma tissues than in paired noncancerous tissues. PMID: 28103919
  5. Expression levels of TP53BP2, FBXO28, and FAM53A genes were associated with patient survival specifically in ER-positive, TP53-mutated tumors. PMID: 28179588
  6. These data together implicated a critical impact of MiR-205/ASPP2 on promoting epithelial-mesenchymal transition. PMID: 27929537
  7. ASPP2 is a key regulator of BECN1-dependent autophagy. PMID: 27929538
  8. Our results suggest that Gal-1 and ASPP2 functionally compete in nanocluster for active Ras on the plasma membrane. ASPP2 dominates the biological outcome, thus switching from a Gal-1 supported growth-promoting setting to a senescence inducing and stemness suppressive program in cancer cells. Our results support Ras nanocluster as major integrators of tumour fate decision events. PMID: 27437940
  9. Downregulated expression of ASPP2 is associated with hepatocellular carcinoma. PMID: 27473084
  10. miR-548d-3p/TP53BP2 pathway is critically involved in the proliferation and apoptosis of breast cancer cells and may be new therapeutic target of breast cancer cells. PMID: 26663100
  11. ASPP1/2-PP1 complexes are required for chromosome segregation and kinetochore-microtubule attachments. PMID: 26595804
  12. ASSP2 expression is lower in cancerous tissues and decreased ASPP2 lead to higher cancer cells proliferation and autophagic flux, which contribute to the gemcitabine resistance. PMID: 26438046
  13. this study revealed a novel function of ASPP2 in modulating autophagy and apoptosis. PMID: 25534115
  14. ASPP2 may participate in the lipid metabolism of non-alcoholic steatohepatitis and attenuate liver failure. PMID: 25256142
  15. These results identify previously uncharacterized regions distributed throughout the protein sequence of ASPP2 as determinants of CagA binding, providing mechanistic insight into apoptosis reprogramming by CagA. PMID: 25963096
  16. ASPP2 directly induces the dephosphorylation and activation of junctional YAP PMID: 25360797
  17. CHOP is critical for mediating ASPP2-induced autophagic apoptosis by decreasing Bcl-2 expression and maintaining nuclear ASPP2-Bcl-2 complexes. PMID: 25032846
  18. ASPP1/2 interacted with centrosome linker protein C-Nap1. Co-depletion of ASPP1 and ASPP2 inhibited re-association of C-Nap1 with centrosome at the end of mitosis. PMID: 25660448
  19. these observations reveal that Itch and Yap1 have antagonistic roles in the regulation of ASPP2 protein stability through competing post-translational regulatory mechanism of ASPP2. PMID: 25436413
  20. phosphorylation of ASPP2 by RAS/MAPK pathway provides a novel link between RAS and p53 in regulating apoptosis PMID: 24312625
  21. Attenuated expression of apoptosis stimulating protein of p53-2 (ASPP2) in human acute leukemia is associated with therapy failure. PMID: 24312201
  22. higher rate Helicobacter pylori infection, an increased expression of inhibitor of apoptosis stimulating protein of p53 (iASPP), and decreased expression of apoptosis-stimulating of p53 protein 2(ASPP2) was present in gastric cancer PMID: 23528480
  23. our studies demonstrate the role of Siah2 in regulation of tight junction integrity and cell polarity under hypoxia, through its regulation of ASPP2 stability. PMID: 23644657
  24. Disruption of CagA and ASPP2 binding alters the function of ASPP2 and leads to the decreased survival of H. pylori-infected cells PMID: 24474782
  25. FIH-1 depletion did lead to impaired binding of Par-3 to ASPP2. PMID: 23606740
  26. We also unveiled a possible mechanistic link between ASPP2 and Csk/Src signaling pathway, implicating the multiple cellular functions of ASPP2. PMID: 23671128
  27. ASPP1 and ASPP2 cooperate with RAS to enhance p53-induced apoptosis suggests that loss of ASPP1 or ASPP2 expression may be a frequent event in human cancers with mutant RAS. PMID: 23392125
  28. When the Px(T)PxR motif is deleted or mutated via insertion of a phosphorylation site mimic (T311D), PP-1c fails to bind to all three ASPP proteins, ASPP1, ASPP2 and iASPP. PMID: 23088536
  29. ASPP2 binds to Ras-GTP at the plasma membrane and stimulates Ras-induced signaling and pERK1/2 levels via promoting Ras-GTP loading, B-Raf/C-Raf dimerization, and C-Raf phosphorylation. PMID: 23248303
  30. the mRNA expression of ASPP1 and ASPP2 was frequently dowregulated in tumor tissues, and this decreased significantly in samples expressing wild-type p53 PMID: 22552744
  31. Methylation in the promoter region of ASPP2 gene was not detected in lung cancer or adjacent non-neoplastic lung tissue. PMID: 22169642
  32. Helicobacter pylori cytotoxin-associated gene A (CagA) subverts the apoptosis-stimulating protein of p53 (ASPP2) tumor suppressor pathway of the host PMID: 21562218
  33. PP1A and ASPP2 play a critical role in promoting TAZ function by antagonizing the LATS kinase through TAZ dephosphorylation. PMID: 21189257
  34. study was to determine localization pattern of ASPP-2 in a variety of normal and malignant human tissues; study indicates that ASPP-2 has a specific distribution pattern within tissues and cells in a way that appears to be related to differentiation PMID: 21183427
  35. ASPP2(ANK-SH3) binds NFkappaB(p65) in a similar manner to its natural inhibitor IkappaB, suggesting a possible novel role for ASPP2 as an NFkappaB inhibitor PMID: 19507243
  36. ASPP1 and ASPP2 genes are frequently down-regulated by DNA methylation in HBV-positive hepatocellular carcinoma, which may play important roles in the development of HCC PMID: 20034025
  37. ASPP2 is a negative regulator of the neddylation pathway through specific interaction with APP-BP1 and suggest that dysfunction of the APP-BP1 interaction with APP may be one cause of Alzheimer's disease PMID: 12694406
  38. TP53BP2 encodes two mRNA species, either with (53BP2) or without exon 3 (ASPP2), by alternative splicing in various cell lines and tissues PMID: 14766226
  39. downstream of E2F, suggesting that it functions as a common link between the p53/p73 and Rb/E2F apoptotic pathways PMID: 15592436
  40. target of E2F transcription factor PMID: 15731768
  41. ASPP2 CpG island aberrant methylation could be one molecular and genetic alteration in wild-type p53 tumours. PMID: 15757645
  42. results suggest that the tumor protein p53 binding protein(TP53BP2) locus is associated with susceptibility to gastric cancer in the Korean population PMID: 15986435
  43. ASPP2/(53BP2L) expression regulation by proteasomal degradation modulates p53 apoptotic function PMID: 16091363
  44. In this study we explored the effect of NF-kappaB activation elicited by a physiological NF-kappaB inducer, interleukin-1beta (IL-1beta), and anti-apoptotic Bcl-2 family proteins on the 53BP2S-mediated apoptosis PMID: 16098144
  45. role of ASPP1, ASPP2, and iASPP as apoptotic specific regulators of p53 [review] PMID: 16139958
  46. suggest a possible role for the N-termini of ASPP proteins in binding to other proteins in the apoptotic response network PMID: 17594908
  47. 53BP2S interacts and modulates the insulin signals mediated by insulin receptor substrates. PMID: 17965023
  48. identifies the interaction sites of Bcl-2 and its homologues with ASPP2 PMID: 18719108
  49. bcl2 -938C/C genotype has worse prognosis and lower survival in patients with renal cell carcinoma. In addition, the bcl2 -938C/A single nucleotide polymorphism was shown to be an independent adverse prognostic factor for renal cell carcinoma PMID: 19539330

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Subcellular Location Cytoplasm, perinuclear region. Nucleus. Note=Predominantly found in the perinuclear region. Some small fraction is nuclear. Sequester in the cytoplasm on overexpression of DDX42.
Protein Families ASPP family
Tissue Specificity Widely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocyte. Reduced expression in breast carcinomas expressing a wild-type TP53 protein. Overexpressed in lung cancer cell lines.
Database Links

HGNC: 12000

OMIM: 602143

KEGG: hsa:7159

STRING: 9606.ENSP00000341957

UniGene: Hs.523968

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