Recombinant Human BCL2/adenovirus E1B 19 kDa protein-interacting protein 2 (BNIP2)

Code CSB-YP615541HU
MSDS
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Source Yeast
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Code CSB-EP615541HU
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Source E.coli
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Code CSB-EP615541HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP615541HU
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Source Baculovirus
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Code CSB-MP615541HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
BNIP2
Uniprot No.
Alternative Names
BCL2/adenovirus E1B 19 kDa protein interacting protein 2; BCL2/adenovirus E1B 19 kDa protein-interacting protein 2; BCL2/adenovirus E1B 19kDa interacting protein 2; BNIP 2 ; Bnip2; BNIP2_HUMAN; NIP2
Species
Homo sapiens (Human)
Expression Region
1-314
Target Protein Sequence
MEGVELKEEW QDEDFPIPLP EDDSIEADIL AITGPEDQPG SLEVNGNKVR KKLMAPDISL TLDPSDGSVL SDDLDESGEI DLDGLDTPSE NSNEFEWEDD LPKPKTTEVI RKGSITEYTA AEEKEDGRRW RMFRIGEQDH RVDMKAIEPY KKVISHGGYY GDGLNAIVVF AVCFMPESSQ PNYRYLMDNL FKYVIGTLEL LVAENYMIVY LNGATTRRKM PSLGWLRKCY QQIDRRLRKN LKSLIIVHPS WFIRTLLAVT RPFISSKFSQ KIRYVFNLAE LAELVPMEYV GIPECIKQVD QELNGKQDEP KNEQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Implicated in the suppression of cell death. Interacts with the BCL-2 and adenovirus E1B 19 kDa proteins.
Gene References into Functions
  1. BNIP-2 is a kinesin-1 adapter involved in vesicular transportation in the cytoplasm and that association with cargos depends on interaction of the CRAL-TRIO domain with membrane phosphatidylserine. PMID: 25472445
  2. BNIP-2 directly interacted with the motor and tail domains of KIF5B via its BCH domain. PMID: 25378581
  3. Data imply that SIRT1 may mediate MPP(+)-induced cytotoxicity, possibly through the regulation of BNIP2. PMID: 21130087
  4. Inhibition of BNIP-2 expression did not affect the susceptibility to NK cell-mediated killing. PMID: 20704564
  5. Transfection of the bnip2 cDNA in neuroblastoma cell line results in massive cell death counteracted by Bcl-2 overexpression; bnip2 is the mediator of the anti-apoptotic activity of estrogens in a variety of cells PMID: 11744098
  6. BNIP-2 has a role in regulating cell dynamics with a novel Rho GTPase-activating protein, BPGAP1 PMID: 12944407
  7. BNIP-2 in vivo induces cell dynamics by recruiting Cdc42 PMID: 15652341
  8. Our results suggest that the caspase-mediated cleavage of BNIP-2 and BNIP-XL could result in the release of the BCH domain or smaller fragments that are crucial for their proapoptotic activities. PMID: 17961507

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Subcellular Location
Cytoplasm. Cytoplasm, perinuclear region. Note=Localizes to the nuclear envelope region and to other cytoplasmic structures.
Database Links

HGNC: 1083

OMIM: 603292

KEGG: hsa:663

STRING: 9606.ENSP00000267859

UniGene: Hs.592515

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