Recombinant Human Biglycan(BGN)

In Stock
Code CSB-EP002683HU
Size US$1726Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names BGN
Uniprot No. P21810
Research Area Signal Transduction
Alternative Names Bone/cartilage proteoglycan I PG-S1
Species Homo sapiens (Human)
Source E.coli
Expression Region 38-368aa
Target Protein Sequence DEEASGADTSGVLDPDSVTPTYSAMCPFGCHCHLRVVQCSDLGLKSVPKEISPDTTLLDLQNNDISELRKDDFKGLQHLYALVLVNNKISKIHEKAFSPLRKLQKLYISKNHLVEIPPNLPSSLVELRIHDNRIRKVPKGVFSGLRNMNCIEMGGNPLENSGFEPGAFDGLKLNYLRISEAKLTGIPKDLPETLNELHLDHNKIQAIELEDLLRYSKLYRLGLGHNQIRMIENGSLSFLPTLRELHLDNNKLARVPSGLPDLKLLQVVYLHSNNITKVGVNDFCPMGFGVKRAYYNGISLFNNPVPYWEVQPATFRCVTDRLAIQFGNYKK
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 57.2kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA Please contact us to get it.
Still Have Questions? Leave a Message or Start an on-line Chat

Q&A and Customer Reviews

 Q&A
Q:

I have plane to buy the CSB-EP002683hu protein with powder form.
I have several questions:
1st Is it possible to dissolve it into the saline to the concetration on 3ug/2ul?
2nd How long maintaing the activity of protein with dissolved saline buffer?
3rd Let me know the temperature for storage.

A:
Thanks for choosing Cusabio !
1. You can use deionized sterile water to dissolve the protein directly, no need to use the saline, because the protein itself has salt ions before lyophilization.
You can dissolve the protein according to the concetration on 3ug/2ul, you need to observe if the protein is fully dissolved.
If you need to dissolve a lot of the protein, you'd better dissolve according to the method introduced in the COA.
2. Protein Shelf Life
* The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
* So the shelf life of different proteins won't be exactly the same. We haven’t performed detailed research for this at present.
* The shelf life of liquid-form protein will be 6 months around at -20℃/-80℃.
* The shelf life of lyophilized form is 12 months around at -20℃/-80℃.
* The shelf life of dissolved recombinant protein is 6 months around at -20℃/-80℃. It refers that customer dissolves protein immediately using sterile water once receiving the item, and then add glycerol with certain final concentration, and finally store at -20℃/-80℃, and then the reconstituted protein can be stored for six months. If customers use the protein immediately, then they don't need to add glycerol, and the protein can be stored for about one week at 4℃.
* The above shelf lives are concluded by our current storage experience. The specific shelf life will depend on specific protein.
3.If you don't use the protein immediately and want to store the protein after dissolving it, you'd better add glycerol with certain final concentration, and finally store at -20℃/-80℃, and then the dissolved protein can be stored for six months.
Sorry, we haven't tested the activity of this protein, we can't 100% guarantee it's active.
We just tell you the proper condition and operation to store this protein, not how to maintain the activity, because we haven't tested the activity.
 Customer Reviews

Target Data

Function May be involved in collagen fiber assembly.
Involvement in disease Meester-Loeys syndrome (MRLS); Spondyloepimetaphyseal dysplasia, X-linked (SEMDX)
Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families Small leucine-rich proteoglycan (SLRP) family, SLRP class I subfamily
Tissue Specificity Found in several connective tissues, especially in articular cartilages.
Database Links

HGNC: 1044

OMIM: 300106

KEGG: hsa:633

STRING: 9606.ENSP00000327336

UniGene: Hs.821

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