Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

IBSP

Uniprot No.

P21815

Research Area

others

Alternative Names

BNSP; Bone sialoprotein 2; Bone sialoprotein II; BSP; BSP II; BSPII; Cell binding sialoprotein; Cell-binding sialoprotein; IBSP; Integrin binding sialoprotein; Integrin-binding sialoprotein; SIAL_HUMAN; SPII

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

129-281aa

Target Protein Sequence

AIQLPKKAGDITNKATKEKESDEEEEEEEEGNENEESEAEVDENEQGINGTSTNSTEAENGNGSSGGDNGEEGEEESVTGANAEDTTETGRQGKGTSKTTTSPNGGFEPTTPPQVYRTTSPPFGKTTTVEYEGEYEYTGANEYDNGYEIYESE
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

32.4kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Unlock the potential of Bone Sialoprotein 2 (BSP2) in your research with our premium Recombinant Human IBSP protein. BSP2, also known as Integrin-Binding Sialoprotein, is an essential component of the extracellular matrix in bone and dentin, playing a crucial role in the process of bone mineralization, cell adhesion, and integrin-mediated signaling pathways. BSP2 is a key protein in the study of bone metabolism, osteoporosis, and dental pathologies, making it an indispensable tool for researchers in these fields.

Our Recombinant Human IBSP protein is expressed in E. coli, ensuring a consistent and reliable product for your research needs. The partial protein length, covering the expression region from 129-281aa, maintains the crucial functional domains of BSP2. An N-terminal 6xHis-SUMO tag has been added to our recombinant IBSP protein to enable straightforward protein purification and detection. With a purity greater than 90% as determined by SDS-PAGE, our Recombinant Human IBSP protein is supplied as a lyophilized powder, providing you with a dependable and effective tool for your bone metabolism and integrin-mediated signaling research endeavors.

Target Background

Function

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment.

Gene References into Functions

These data indicate that secretome derived from salivary gland cancer cells can influence the expression of two potential biomarkers of oral cancer-namely, bone sialoprotein (BSP) and dentin sialoprotein (DSP)-in normal salivary gland cells. PMID: 27881474
In conclusion, serum levels of BSP, ALP, ICTP, and PSA increased in patients with bone metastases, and combined detection of all markers could improve the positive-predictive value. PMID: 27323113
Preameloblast-Derived Factors Mediate Osteoblast Differentiation of Human Bone Marrow Mesenchymal Stem Cells by Runx2-Osterix-BSP Signaling. PMID: 26413977
Two significant SNPs within IBSP, rs1054627 and rs17013181, were associated with BMD and postmenopausal osteoporosis by the two-stage strategy, and rs17013181 was also significantly associated with serum IBSP levels. PMID: 26568273
Our results suggest that SSEA-4 is a specific cell surface antigen that can be used to identify dental pulp stem cells. PMID: 22266579
the strong correlation between bone sialoprotein and OPN and papillary thyroid carcinoma suggests a role for BSP and OPN in calcification and tumor progression of papillary thyroid carcinoma PMID: 25973097
oxidized low-density lipoprotein-induced expression dependent on Runx2 expression PMID: 25504218
Bone sialoprotein could be a key mediator of the hypertrophic chondrocytes-induced angiogenesis of osteoarthritis. PMID: 24530278
Current evidence demonstrates that BSP and OPN, play significant roles in bone metastasis of osteotropic malignancies derived from breast, prostate, lung, thyroid, and multiple myeloma. [review] PMID: 24071501
High expression of bone sialoprotein in breast neoplasms was associated with cytokeratin-positive cells in bone marrow, but not with lymph node metastasis. PMID: 23726130
results indicate that FGF2 increases BSP transcription by targeting the FRE and AP1 elements in the proximal promoter of the human BSP gene. PMID: 23485603
BSP silencing decreased the integrin alphavbeta3 and beta3 levels, in turn inhibiting cell migration and invasion and decreasing the ability of the cells to metastasize to bone. PMID: 23667544
IBSP mRNA is over expressed in carotid atheroma plaque (3.74 fold, p = 1.41E-09) in an intraindividual comparison. PMID: 23314561
High BSP expression occurs in a significant subset of high-grade glioma patients and predicts a poorer outcome PMID: 23119009
HTRA1 has a central role in osteogenesis through modification of proteins within the extracellular matrix, in particular, ibsp. PMID: 22865667
human primary cementoblasts subjected to compression and IL-1beta stimulation impeded BSP and CEMP-1 expression, proteins that are associated with cementogenesis. PMID: 22349547
OPN plasma levels are associated with the genetic polymorphisms in integrin-binding sialoprotein gene locus (IBSP) PMID: 20967421
BSP protein expression in the primary resected non-small-cell lung cancer is strongly associated with bone metastasis and could be used to identify high-risk patients. PMID: 19376608
RT-PCR analysis of human bone marrow stromal cells during osteogenesis in vitro: the mRNA levels of bone morphogenetic protein-2 (BMP-2), bone sialoprotein-II (BSP), osteopontin (OP) and cbfa-1 increased with culture time in osteogenic medium. PMID: 11968014
has RGD sequence, affinity to collagen, and induces mineral crystal formation PMID: 11979972
Osteoblast-related transcription factors Runx2 (Cbfa1/AML3) and MSX2 mediate the expression of bone sialoprotein in human metastatic breast cancer cells. PMID: 12750290
BSP is expressed in breast and prostate cancer and has a role as a stimulator of bone mineralisation PMID: 14524533
The time course of the expression of BSP wss visualized after dental implnt implatation in mandibular bone fibroblasts. PMID: 15795688
Data show that RUNX2 is a direct regulator of bone sialoprotein in osteoblasts and that it functions in cooperation with DLX5 or a related factor to activate osteoblast-specific gene expression. PMID: 16000302
Bone sialoprotein is involved in migration of bone marrow stromal cells through Matrigel and collagen barriers. PMID: 16995818
bone sialoprotein expression in the primary resected NSCLC is strongly associated with BM progression and could be useful in identifying high-risk patients who could benefit from novel modalities of surveillance and preventive treatment PMID: 17050866
May be a prognostic marker for bone metastasis in breast cancer. PMID: 17213971
Runx2 and HDAC3 repress BSP gene expression and that this repression is suspended upon osteoblastic cell differentiation. PMID: 17956871
has an angiogenic capacity; important in the differentiation of osteoblasts, bone matrix mineralization and tumor metastasis [review] PMID: 18302613
PTH stimulates human BSP gene transcription by targeting the two cAMP response elements in the promoter of the human BSP gene. PMID: 19127545
Studies do not support a role for BSP in promoting metastasis through interactions with pro-MMP-2. PMID: 19386107
cooperative mechanisms by which BSP can enhance specific factors associated with a metastatic phenotype in tumor cell lines, an effect that is increased by circulating TGF-beta1 and EGF. PMID: 19492334
Eight threonines modified by O-glycans were identified, leaving the C terminus of the protein free of glycans. The recombinant protein showed similar secondary structures as bone-derived BSP PMID: 11459848

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Subcellular Location

Secreted.

Database Links

HGNC: 5341

OMIM: 147563

KEGG: hsa:3381

STRING: 9606.ENSP00000226284

UniGene: Hs.518726

Code	CSB-EP010945HU(A4)
Abbreviation	Recombinant Human IBSP protein, partial
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Bone sialoprotein 2 (IBSP),Partial

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