Recombinant Human CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1 (ST3GAL1), partial

Product Details

Purity

≥85% (SDS-PAGE)

Target Names

ST3GAL1

Uniprot No.

Alternative Names

3-GalNAc-alpha-2; 3-sialyltransferase 1; 3-sialyltransferase; 3-ST 1; Alpha 2; Alpha 23 ST; Beta galactoside alpha 23 sialyltransferase; Beta-galactoside alpha-2; beta-galactoside alpha-2,3-sialyltransferase 1; CMP N acetylneuraminate beta galactosamide alpha 23 sialyltransferase; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2; DKFZp666E036; DKFZp779K2051; FLJ36548; Gal beta 13 GalNAc alpha 23 sialyltransferase; Gal NAc6S; Gal-beta-1; Gal-NAc6S; MGC9183; OTTHUMP00000226912; OTTHUMP00000226913; SIA4A_HUMAN; Sialyltransferase 4A; SIAT4-A; SIAT4A; SIATFL; ST3Gal I; ST3GAL1; ST3GalA; ST3GalA.1; ST3GalI; ST3GalIA; ST3O

Species

Homo sapiens (Human)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

A beta-galactoside alpha2-3 sialyltransferase involved in terminal sialylation of glycoproteins and glycolipids. Catalyzes the transfer of sialic acid (N-acetyl-neuraminic acid; Neu5Ac) from the nucleotide sugar donor CMP-Neu5Ac onto acceptor Galbeta-(1->3)-GalNAc-terminated glycoconjugates through an alpha2-3 linkage. Adds sialic acid to the core 1 O-glycan, Galbeta-(1->3)-GalNAc-O-Ser/Thr, which is a major structure of mucin-type O-glycans. As part of a homeostatic mechanism that regulates CD8-positive T cell numbers, sialylates core 1 O-glycans of T cell glycoproteins, SPN/CD43 and PTPRC/CD45. Prevents premature apoptosis of thymic CD8-positive T cells prior to peripheral emigration, whereas in the secondary lymphoid organs controls the survival of CD8-positive memory T cells generated following a successful immune response. Transfers sialic acid to asialofetuin, presumably onto Galbeta-(1->3)-GalNAc-O-Ser. Sialylates GM1a, GA1 and GD1b gangliosides to form GD1a, GM1b and GT1b, respectively.

Gene References into Functions

Study demonstrates that ST3GalI regulates ovarian cancer cell migration and peritoneal dissemination via EGFR signaling. PMID: 28423672
Describe differential expression profiles of alpha-2, 3-sialyltransferases (ST) and miR-4701-5p in three pairs of chronic myeloid leukemia (CML) cell lines and 48 clinical samples of bone marrow mononuclear cells from CML patients. miR-4701-5p directly targeted ST3GAL1 to reduce CML cells resistance to multiple chemotherapeutics in vitro. PMID: 27088512
rs113350588 and rs1048479 may alter the function of ST3GAL1. The GC haplotype was associated with an increased risk of death in subjects with influenza. The AT haplotype was associated with an increased risk of severe disease and death. PMID: 26436774
ST3GAL1-associated transcriptomic program portends poor prognosis in glioma patients with higher tumor grades.ST3Gal1-regulated self-renewal traits are crucial to the sustenance of glioblastoma multiforme growth. PMID: 26547933
Results show that ST3Gal1 uses GM130-GRASP65 and giantin, whereas C2GnT-L uses only giantin for Golgi targeting and defective giantin dimerization in PC-3 and DU145 prostate cancer cells causes fragmentation of the Golgi and prevents its targeting. PMID: 25086069
NMIIA is the master regulator of Golgi fragmentation induced by heat shock or inhibition/depletion of HSP70/90 through interaction with gylosyltransferases. PMID: 23990450
Down-regulation of ST3GalI is correlated with breast cancer PMID: 22534569
Over-expression of ST3Gal-I promotes mammary tumorigenesis. PMID: 20534593
ST3Gal.I plays the major role in the sialylation of the T antigen in bladder cancer. PMID: 19811634
Although the human ST3Gal I has four N-glycan attachment sites in its catalytic domain that are potentially glycosylated, none necessary for enzyme activity, but N-glycosylation contributes to the folding and trafficking of the enzyme. PMID: 14722111
ST3Gal-I inactivation or enzymatic removal of its product renders CD8+ T cells, but not CD4+ T cells, susceptible to apoptosis by differential cross-linking of O-glycoproteins in the absence of interleukin-2 and T-cell receptor (TCR) signaling. PMID: 17101770
evidence of the association of bipolar disorder with SIAT4A was seen PMID: 18180429

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Subcellular Location

Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Golgi apparatus, trans-Golgi network membrane; Single-pass type II membrane protein. Secreted.

Protein Families

Glycosyltransferase 29 family

Tissue Specificity

Expressed in several tissues. Highest expression in lung, liver, skeletal muscle, kidney, pancreas, spleen and placenta.

Database Links

HGNC: 10862

OMIM: 607187

KEGG: hsa:6482

STRING: 9606.ENSP00000318445

UniGene: Hs.374257

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Recombinant Human CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1 (ST3GAL1), partial

Product Details

Customer Reviews and Q&A

Target Background

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