Recombinant Human CMRF35-like molecule 8(CD300A),partial

Code CSB-YP887023HU
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Source Yeast
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Code CSB-EP887023HU
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Source E.coli
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Code CSB-EP887023HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP887023HU
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Source Baculovirus
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Code CSB-MP887023HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names CD300A
Uniprot No. Q9UGN4
Alternative Names CD300 antigen-like family member A; CD300a; CD300a antigen; CD300a molecule; CLM-8; CLM8_HUMAN; CMRF 35 H9; CMRF-35-H9; CMRF35-H; CMRF35-H9; CMRF35-like molecule 8; CMRF35H; CMRF35H leukocyte immunoglobulin like receptor; CMRF35H9; IgSF12; Immunoglobulin superfamily member 12; Inhibitory receptor protein 60; IRC1; IRC1/IRC2; IRC2; IRp60; Leukocyte membrane antigen; Mast cell derived paired immunoglobulin like receptor 1; NK inhibitory receptor; Polymeric immunoglobulin receptor 4
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation. Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 but not TRIF through activation of PTPN6.
Gene References into Functions
  1. Results provide an insight into the novel set of paired receptors CD300a and CD300c that are distinctively expressed on CD56(bright) natural killers (NK) cells with varied effector functions and show there differential binding to their ligands phosphatidylethanolamine and phosphatidylserine (PS) resulting in their differential ability to affect CD56(bright) NK cell functions. PMID: 27040328
  2. High CD300A expression is associated with diffuse large B-cell lymphoma. PMID: 26435477
  3. US3 protein kinase of pseudorabies virus triggers the binding of the inhibitory natural killer cell receptor CD300a to the surface of the infected cell, and protects of virus infected cells against natural killer cell-mediated lysis. PMID: 26581992
  4. Overall, these data indicate that CD300a is a novel Dengue virus binding receptor that recognizes phosphatidylethanolamine and phosphatidylserine present on virions and enhance infection. PMID: 26468529
  5. Data show that peroxisome proliferator activator receptor delta (PPARdelta/beta) directly regulates CD300a protein in macrophages. PMID: 24958459
  6. CD300a induction by the hypoxic environment represents a mechanism of regulation of monocyte/macrophage pro-inflammatory responses at pathologic sites PMID: 24131792
  7. interaction with phosphatidylserines inhibits IgE/FcepsilonRI-dependent anaphylactic basophil degranulation PMID: 24815424
  8. The inhibitory receptor CD300a is regulator of monocytes transendothelial migration. PMID: 24058511
  9. SHP-1, but not SHP-2 or the src homology 2 domain containing inositol 5' phosphatase SHIP, was utilized by CD300a for its inhibitory activity. These studies provide new insights into the function of CD300a in tuning T and B cell responses. PMID: 22537350
  10. CD300a is expressed on human peripheral blood basophils and rapidly up-regulated upon cross-linking of IgE/Fc-epsilon-RI and suppresses anaphylactic degranulation. PMID: 22173928
  11. our results indicate that phosphatidylethanolamine and phosphatidylserine are ligands for CD300a, and that this interaction plays an important role in regulating the removal of dead cells. PMID: 22302738
  12. These results indicated that CD300a is a new phosphatidylserine receptor. PMID: 22185693
  13. CD300a blocked TLR4-mediated and TLR9-mediated expression of pro-inflammatory mediators without affecting TLR3-mediated events. PMID: 22043923
  14. Down-regulation of CD300a is associated with HIV infection. PMID: 21482706
  15. CD300a is an inhibitory receptor expressed by human natural killer (NK) cells, but surprisingly, not all NK clones are inhibited in a CD300a-dependent manner. PMID: 20656921
  16. CD300a(+) human Th1 cells tend to be polyfunctional and after stimulation up-regulate Eomes. PMID: 20498708
  17. CMRF35A and CMRF35H have roles in immunoglobulin signaling; CMRF35H is an inhibitory molecule [review] PMID: 12456026
  18. IRp60 is constitutively expressed on mast cells but is down-regulated by eosinophil major basic protein and eosinophil-derived neurotoxin. Cross-linking of IRp60 led to inhibition of IgE-induced degranulation and stem cell factor-mediated survival PMID: 16339535
  19. Human neutrophils from peripheral blood express the ITIM containing CD300a (also known as IRp60 and CMRF-35H) receptor. Co-ligation of CD300a with (FcgammaRIIa) activation receptor inhibited CD32a mediated signalling. PMID: 17588661
  20. CD300a as a novel regulator of Kit in human MC and suggest roles for this receptor as a suppressor of Kit signaling in MC-related disorders. PMID: 18424727

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Subcellular Location Cell membrane; Single-pass type I membrane protein.
Protein Families CD300 family
Tissue Specificity Expressed not only by natural killer (NK) cells but also by T-cell subsets, B-cells, dendritic cells, mast cells, granulocytes and monocytes.
Database Links

HGNC: 19319

OMIM: 606790

KEGG: hsa:11314

STRING: 9606.ENSP00000353259

UniGene: Hs.9688

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