Recombinant Human COMM domain-containing protein 5 (COMMD5) is produced in E.coli and includes the full length of the mature protein, covering amino acids 2 to 224. An N-terminal 6xHis-tag has been added to help with purification and detection. SDS-PAGE analysis shows the protein achieves a purity level greater than 85%. The product is intended for research use only and appears to perform reliably in laboratory settings.
COMMD5 belongs to the COMM domain-containing protein family, which seems to play a role in various cellular processes. The protein is involved in intracellular signaling pathways and may contribute to regulating protein trafficking and degradation. Studying this protein is important for understanding cellular homeostasis and could provide insights into the broader functions of COMM domain-containing proteins in human biology.
Potential Applications
Note: The applications listed below are based on what we know about this protein's biological functions, published research, and experience from experts in the field. However, we haven't fully tested all of these applications ourselves yet. We'd recommend running some preliminary tests first to make sure they work for your specific research goals.
1. Protein-Protein Interaction Studies
This recombinant COMMD5 protein can be used in pull-down assays to identify and characterize potential binding partners within the COMMD protein family or other cellular proteins. The N-terminal 6xHis tag allows for immobilization on nickel-based resins during affinity purification experiments. Co-immunoprecipitation studies using this protein as bait may help reveal the molecular networks involving COMMD5 in cellular processes.
2. Antibody Development and Validation
The purified recombinant COMMD5 protein works well as an antigen for generating specific antibodies against human COMMD5. With its high purity level (>85%), there's likely to be minimal cross-reactivity during immunization protocols. Researchers can validate generated antibodies using this recombinant protein in Western blot, ELISA, and other immunoassays to confirm specificity and determine optimal working concentrations.
3. Structural and Biophysical Characterization
This full-length mature COMMD5 protein can be used for structural studies including X-ray crystallography, NMR spectroscopy, or cryo-electron microscopy to understand its three-dimensional architecture. Biophysical techniques such as dynamic light scattering, circular dichroism spectroscopy, and analytical ultracentrifugation may provide insights into protein folding, stability, and oligomerization states under various buffer conditions.
4. Biochemical Assay Development
The recombinant COMMD5 protein works as a standard or control in biochemical assays designed to study COMMD protein function. The His-tag makes purification and quantification straightforward for dose-response studies and enzyme kinetics experiments. This protein can also be used to develop and optimize detection methods for endogenous COMMD5 in cell lysates or tissue samples.
