Recombinant Human CREB3 regulatory factor (CREBRF)

Code CSB-YP815547HU
MSDS
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Source Yeast
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Code CSB-EP815547HU
MSDS
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Source E.coli
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Code CSB-EP815547HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP815547HU
MSDS
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Source Baculovirus
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Code CSB-MP815547HU
MSDS
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Source Mammalian cell
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Product Details

Purity
≥85% (SDS-PAGE)
Target Names
CREBRF
Uniprot No.
Alternative Names
adult retina protein ; C5orf41; Chromosome 5 Open Reading Frame 41; CREB3 regulatory factor; CREBRF; CRERF_HUMAN; Hypothetical protein; LRF; Luman recruitment factor
Species
Homo sapiens (Human)
Expression Region
1-639
Target Protein Sequence
MPQPSVSGMD PPFGDAFRSH TFSEQTLMST DLLANSSDPD FMYELDREMN YQQNPRDNFL SLEDCKDIEN LESFTDVLDN EGALTSNWEQ WDTYCEDLTK YTKLTSCDIW GTKEVDYLGL DDFSSPYQDE EVISKTPTLA QLNSEDSQSV SDSLYYPDSL FSVKQNPLPS SFPGKKITSR AAAPVCSSKT LQAEVPLSDC VQKASKPTSS TQIMVKTNMY HNEKVNFHVE CKDYVKKAKV KINPVQQSRP LLSQIHTDAA KENTCYCGAV AKRQEKKGME PLQGHATPAL PFKETQELLL SPLPQEGPGS LAAGESSSLS ASTSVSDSSQ KKEEHNYSLF VSDNLGEQPT KCSPEEDEED EEDVDDEDHD EGFGSEHELS ENEEEEEEEE DYEDDKDDDI SDTFSEPGYE NDSVEDLKEV TSISSRKRGK RRYFWEYSEQ LTPSQQERML RPSEWNRDTL PSNMYQKNGL HHGKYAVKKS RRTDVEDLTP NPKKLLQIGN ELRKLNKVIS DLTPVSELPL TARPRSRKEK NKLASRACRL KKKAQYEANK VKLWGLNTEY DNLLFVINSI KQEIVNRVQN PRDERGPNMG QKLEILIKDT LGLPVAGQTS EFVNQVLEKT AEGNPTGGLV GLRIPTSKV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Acts as a negative regulator of the endoplasmic reticulum stress response or unfolded protein response (UPR). Represses the transcriptional activity of CREB3 during the UPR. Recruits CREB3 into nuclear foci.
Gene References into Functions
  1. The presence of each additional CREBRF rs373863828 A allele is associated with increased BMI yet reduced odds of type 2 diabetes in adults of Maori and Pacific (Polynesian) ancestry living in Aotearoa/New Zealand. PMID: 29721634
  2. We found that CREBRF and TRIM2 mRNA were significantly upregulated by rHDL, particularly in response to its phospholipid component 1-palmitoyl-2-linoleoyl-phosphatidylcholine, however, protein expression was not significantly altered. PMID: 29958463
  3. The rs373863828-A allele (a missense variant of the CREBRF gene) may not directly affect the level of serum HDL-cholesterol independent of BMI. PMID: 29877158
  4. CREBRF promotes the proliferation of human gastric cancer cells via the AKT signaling pathway. PMID: 29729692
  5. The rs373863828-A allele was not found in both AN-speaking and non-AN-speaking Melanesians living in Papua New Guinea. Our results suggest that rs373863828-A of CREBRF, a promising thrifty variant, arose in recent ancestors of AN-speaking Polynesians. PMID: 28405013
  6. Missense Mutation in CREBRF gene is associated with obesity. PMID: 27455349
  7. Findings provide new insight into the molecular mechanisms underlying hypoxia-induced glioblastoma cell autophagy and indicate that the hypoxia/CREBRF/CREB3/ATG5 pathway plays a central role in malignant glioma progression. PMID: 27278737

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Subcellular Location
Nucleus. Note=Colocalizes with CREB3 in nuclear foci.
Protein Families
BZIP family, CREBRF subfamily
Database Links

HGNC: 24050

KEGG: hsa:153222

STRING: 9606.ENSP00000296953

UniGene: Hs.484195

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