Recombinant Human Calcium-activated potassium channel subunit beta-1(KCNMB1),partial

Code CSB-YP621958HU
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Source Yeast
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Code CSB-EP621958HU
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Source E.coli
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Code CSB-EP621958HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP621958HU
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Source Baculovirus
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Code CSB-MP621958HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names KCNMB1
Uniprot No. Q16558
Alternative Names BK channel beta subunit; BK channel subunit beta 1; BK channel subunit beta-1; BKbeta; BKbeta1; Calcium activated potassium channel subfamily M subunit beta 1; Calcium activated potassium channel subunit beta 1; Calcium activated potassium channel subunit beta; Calcium-activated potassium channel; Calcium-activated potassium channel subunit beta; Calcium-activated potassium channel subunit beta-1; Charybdotoxin receptor subunit beta 1; Charybdotoxin receptor subunit beta-1; Hbeta1; hslo beta; K(VCA)beta 1; K(VCA)beta; K(VCA)beta-1; KCMB1_HUMAN; KCNMB 1; Kcnmb1; Large conductance Ca2+ activated K+ channel beta 1 subunit; Maxi K channel beta subunit; Maxi K channel subunit beta 1; Maxi K channel subunit beta-1; Potassium large conductance calcium activated channel subfamily M beta member 1; Slo beta 1; SLO beta; Slo-beta; Slo-beta-1; subfamily M subunit beta-1
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Increases the apparent Ca(2+)/voltage sensitivity of the KCNMA1 channel. It also modifies KCNMA1 channel kinetics and alters its pharmacological properties. It slows down the activation and the deactivation kinetics of the channel. Acts as a negative regulator of smooth muscle contraction by enhancing the calcium sensitivity to KCNMA1. Its presence is also a requirement for internal binding of the KCNMA1 channel opener dehydrosoyasaponin I (DHS-1) triterpene glycoside and for external binding of the agonist hormone 17-beta-estradiol (E2). Increases the binding activity of charybdotoxin (CTX) toxin to KCNMA1 peptide blocker by increasing the CTX association rate and decreasing the dissociation rate.
Gene References into Functions
  1. Expression of vascular large-conductance calcium-activated potassium channel Kcnmb1 is regulated by Nrf2 signaling. PMID: 28429615
  2. The study demonstrates that both transmembrane domains of BKbeta1 are required to provide the characteristic ion current phenotype of beta1-containing BK channels. PMID: 25275635
  3. Data show that two lysine residues that are unique to the N terminus of calcium-activated potassium channel subunit beta-1 were identified to be sufficient for voltage-sensor modulation. PMID: 25825713
  4. To determine the relationship between atherosclerosis and KCNMB1, we studied some atherogenic factors affecting vascular tone. Blood of atherosclerotic patients shows increased concentration of 7-ketocholesterol, possibly a harmful lipid to blood vessels. PMID: 25576871
  5. Bisphenol A activates BK alpha via an extracellular site and that Bisphenol A-sensitivity is increased by the beta1 subunit. PMID: 24476761
  6. In large-conductance calcium-dependent potassium channels, beta1 and beta2 subunits alter the voltage sensing domain-cytosolic domain interface. PMID: 23825428
  7. The results indicate that the beta1-subunit can form a tripartite complex with TP and MaxiKalpha, has the ability to associate with each protein independently. PMID: 23255603
  8. HIF-1alpha increases KCNMB1 expression in response to hypoxia in human pulmonary artery smooth muscle cells by binding to two hypoxia response elements located at -3,540 to -3,311 of the KCNMB1 promoter PMID: 22114151
  9. Here we found that KCNMB1 common variant Glu65Lys influences glomerular filtration rate across multiple populations of different clinical characteristics and biogeographic ancestries. PMID: 20861615
  10. downregulation of vascular BK-beta(1) expression in diabetes and in high-glucose culture conditions was associated with FOXO-3a/FBXO-dependent increase in BK-beta(1) degradation. PMID: 20966391
  11. Results reveal the first ion channel subunit as a direct target of SRF-MYOCD transactivation, providing further insight into the role of MYOCD as a master regulator of the SMC contractile phenotype. PMID: 19801679
  12. Phosphorylation-dependent functional coupling of hSlo and its hbeta 4 subunit PMID: 11790768
  13. have a reset baroreflex. Variants in the gene (KCNMB1) coding for the BK beta1 subunit are associated with baroreflex function. PMID: 12011654
  14. mesangial cells contain hbeta1, a BK accessory protein, which confers activation of BK by cGMP kinase. PMID: 12670831
  15. Beta1 subunits facilitate gating of BK channels by acting through the Ca2+, but not the Mg2+, activating mechanisms. PMID: 12893878
  16. We conclude that transient membrane current is generated by a mechanism related to the deactivation, and level of prior activation, of glioma BK channels. PMID: 12962281
  17. results reveal the molecular regions in the beta1 and beta2 subunits that determine their differential functional coupling with the pore-forming alpha-subunit PMID: 16446507
  18. identified and characterized single nucleotide polymorphisms in the exons, intron/exon junctions, upstream region and 3' untranslated regions of KCNMB1 using denaturing high-performance liquid chromatography combined with direct DNA sequencing PMID: 17496725
  19. The protective effect of KCNMB1 E65K against hypertension is restricted to blood pressure treatment with beta-blockade. PMID: 18418400
  20. African American male asthmatics carrying the KCNMB1 818T allele (10% of population) are potentially at risk for greater airway obstruction and increased asthma morbidity. PMID: 18535015
  21. The findings suggest that the KCNMB1 Glu65Lys polymorphism associates with reduced systolic and diastolic blood pressure in middle-aged men. PMID: 18854753
  22. The KCNMB1 E65K variant is associated with reduced central pulse pressure. PMID: 19050450
  23. maxi-K(+) beta1-subunit could contribute to vascular dysregulation in severe obstructive sleep apnea syndrome patients. Leukocyte beta1 expression may be independent readout of hypoxemia that could be useful as molecular marker for impending hypertension. PMID: 19057512
  24. Cell membrane stretch and cell volume sensitivity are independently regulated by KCNMB1 (BK) and KCNQ1 channels, respectively. PMID: 19289549
  25. genetic polymorphism shows sex-specific association with asthma severity in male african population PMID: 19295429

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Subcellular Location Membrane; Multi-pass membrane protein.
Protein Families KCNMB (TC 8.A.14.1) family, KCNMB1 subfamily
Tissue Specificity Abundantly expressed in smooth muscle. Low levels of expression in most other tissues. Within the brain, relatively high levels found in hippocampus and corpus callosum.
Database Links

HGNC: 6285

OMIM: 603951

KEGG: hsa:3779

STRING: 9606.ENSP00000274629

UniGene: Hs.484099

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