Recombinant Human Calcium-regulated heat stable protein 1 (CARHSP1)

Code CSB-YP896497HU
MSDS
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Source Yeast
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Code CSB-EP896497HU
MSDS
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Source E.coli
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Code CSB-EP896497HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP896497HU
MSDS
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Source Baculovirus
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Code CSB-MP896497HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CARHSP1
Uniprot No.
Alternative Names
Calcium regulated heat stable protein (24kD); Calcium regulated heat stable protein 1 (24kD); Calcium regulated heat stable protein 1; Calcium-regulated heat stable protein 1; Calcium-regulated heat-stable protein of 24 kDa; Carhsp1; CHSP1_HUMAN; CRHSP-24; CRHSP24; CSDC1
Species
Homo sapiens (Human)
Expression Region
2-147
Target Protein Sequence
SSEPPPPPQ PPTHQASVGL LDTPRSRERS PSPLRGNVVP SPLPTRRTRT FSATVRASQG PVYKGVCKCF CRSKGHGFIT PADGGPDIFL HISDVEGEYV PVEGDEVTYK MCSIPPKNEK LQAVEVVITH LAPGTKHETW SGHVISS
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Binds mRNA and regulates the stability of target mRNA. Binds single-stranded DNA (in vitro).
Gene References into Functions
  1. Increased miR-155 relieves chronic inflammation by a negative feedback loop and plays a protective role during atherosclerosis-associated foam cell formation by signaling through the miR-155-CARHSP1-TNF-alpha pathway. PMID: 26899994
  2. CARHSP1 inhibits hepatic gluconeogenic gene expression via repression of PPARalpha PMID: 21990353
  3. CRHSP-24 participates in oxidative stress response via a dynamic and temporal association between stress granules and processing bodies. PMID: 21177848
  4. Data suggest that CARHSP1 is a TNF-alpha mRNA stability enhancer required for effective TNF-alpha production, demonstrating the importance of both stabilization and destabilization pathways in regulating the TNF-alpha mRNA half-life. PMID: 21078874
Subcellular Location
Cytoplasm. Cytoplasm, P-body. Cytoplasmic granule.
Database Links

HGNC: 17150

OMIM: 616885

KEGG: hsa:23589

STRING: 9606.ENSP00000311847

UniGene: Hs.632184

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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