Recombinant Human Casein kinase II subunit alpha(CSNK2A1)

Code CSB-YP006072HU
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Source Yeast
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Code CSB-EP006072HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP006072HU
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Source Baculovirus
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Code CSB-MP006072HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names CSNK2A1
Uniprot No. P68400
Alternative Names Casein kinase 2 alpha 1 polypeptide; Casein kinase II alpha 1; Casein kinase II alpha 1 subunit; Casein kinase II alpha subunit; Casein kinase II subunit alpha; CK II alpha; CK II; CK2 alpha; CK2 catalytic subunit alpha; CK2A1; CKII; CKIIalpha; CSK21_HUMAN; CSNK2A1
Species Homo sapiens (Human)
Expression Region 1-391
Target Protein Sequence MSGPVPSRAR VYTDVNTHRP REYWDYESHV VEWGNQDDYQ LVRKLGRGKY SEVFEAINIT NNEKVVVKIL KPVKKKKIKR EIKILENLRG GPNIITLADI VKDPVSRTPA LVFEHVNNTD FKQLYQTLTD YDIRFYMYEI LKALDYCHSM GIMHRDVKPH NVMIDHEHRK LRLIDWGLAE FYHPGQEYNV RVASRYFKGP ELLVDYQMYD YSLDMWSLGC MLASMIFRKE PFFHGHDNYD QLVRIAKVLG TEDLYDYIDK YNIELDPRFN DILGRHSRKR WERFVHSENQ HLVSPEALDF LDKLLRYDHQ SRLTAREAME HPYFYTVVKD QARMGSSSMP GGSTPVSSAN MMSGISSVPT PSPLGPLAGS PVIAAANPLG MPVPAAAGAQ Q
Protein Length Full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation. Can also negatively regulate apoptosis. Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3. Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8. Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV. Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB. Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function. Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1. Acts as an ectokinase that phosphorylates several extracellular proteins. During viral infection, phosphorylates various proteins involved in the viral life cycles of EBV, HSV, HBV, HCV, HIV, CMV and HPV. Phosphorylates PML at 'Ser-565' and primes it for ubiquitin-mediated degradation. Plays an important role in the circadian clock function by phosphorylating ARNTL/BMAL1 at 'Ser-90' which is pivotal for its interaction with CLOCK and which controls CLOCK nuclear entry
Gene References into Functions
  1. Casein kinase II, glycogen synthase kinase-3, and Ikaros mediated regulation of leukemia has been summarized. (Review) PMID: 28623166
  2. Our results showcase the potentially valuable functional impact of microRNAs in regulating GG related epilepsy. As CK-2alpha inhibition enhances miR-217 expression, disrupting the miR-217-CK-2alpha interplay through CK-2alpha inhibitors might represent a novel therapeutic strategies aimed at LEAT. PMID: 28840260
  3. The results suggest that CK2alpha promotes migration and invasion of clear cell renal cell carcinoma and therefore could serve as a novel prognostic biomarker and molecular therapeutic target in this type of cancer. PMID: 27906674
  4. The newly designed casein kinase 2 (CK2) Inhibitors were screened on one lung cancer cell line A549, showing low micromolar anti-proliferative activity. PMID: 27491649
  5. CK2 governs the molecular decision between encephalitogenic TH17 cells and protective Treg cells development. PMID: 27555590
  6. Data suggest that casein kinase 2 (CK2) inhibition is a promising approach to blocking beta-catenin in MPNST cells, although combinatorial therapies may be required for maximal efficacy. PMID: 27448963
  7. Results provide evidence that CK2A is critical fro RSV virus replication in human cells. PMID: 27464690
  8. Vascular smooth muscle protein kinase CK2 inhibition suppresses neointima formation via a proline-rich homeodomain-dependent mechanism. PMID: 28927755
  9. we have identified kinases, particularly CK2, associated with the autocrine malignant cell network that may play a central role in sustaining the cytokine network and/or mediating its effects in ovarian cancer. PMID: 26871292
  10. CK2 and AKT display a high degree of cross-regulation of their respective functions, both directly, through physical interaction and phosphorylation, and indirectly, through an intense cross-talk of key downstream effectors, ultimately leading to sustained AKT activation. (Review) PMID: 28373060
  11. Phosphorylation of Ikaros by CK2 impairs Ikaros DNA-binding ability, as well as Ikaros ability to regulate gene expression and function as a tumor suppressor in leukemia. (Review) PMID: 27666503
  12. data implicate CK2 as a regulator of the Th17/Treg axis and Th17 cell maturation and suggest that CK2 could be targeted for the treatment of Th17 cell-driven autoimmune disorders. PMID: 28468969
  13. This study demonstrates that CSNK2A1 and SIRT6 are indicators of poor prognosis for breast carcinomas and that CSNK2A1-mediated phosphorylation of SIRT6 might be involved in the progression of breast carcinoma. PMID: 27746184
  14. Our results disclose a novel interplay between ubiquitin- and phosphorylation-dependent signalling, and represent the first report of a regulatory mechanism for UIM-dependent function. They also suggest that CK2 inhibitors could release the full neuroprotective potential of HSJ1, and deserve future interest as therapeutic strategies for neurodegenerative disease. PMID: 28031292
  15. CK2 inhibition in monocyte-derived dendritic cells led to an enhanced Th2 polarization in the absence of contact sensitizer stimulation. PMID: 27707883
  16. In the present work we have shown a key role for protein kinase CK2 in promoting LSC survival through the modulation of the STAT3, NF-kappaB and AKT/FOXO signaling pathways. We have also shown that CK2 inactivation makes LSCs more sensitive to the chemotherapeutic drug doxorubicin. PMID: 27479180
  17. our findings establish an important regulatory role of CK2alpha on BMI1 phosphorylation and stability and implicate the CK2alpha/BMI1 axis in ovarian cancer. PMID: 28270146
  18. The lack of intermediate species of CK2 alpha2beta2 in solution suggests that the holoenzyme is a strong/stable multimeric complex that does not spontaneously dissociate; a considerable amount of monomer, the active form of CK2, is present in low ionic strength solutions. Multimer assembly appears driven by electrostatic interactions between the CK2alpha P+1 loop and the CK2beta acidic loop. PMID: 28572157
  19. These data support a role for casein kinase 2 in regulation of protein synthesis by downregulating stress granule formation through G3BP1. PMID: 27920254
  20. The invasion and migration of A549 cells were significantly inhibited after the knockdown of CK2alpha expression. PMID: 28442011
  21. Somatic mutations in CSNK2A1 have been implicated in various cancers; however, this is the first study to describe a human condition associated with germline mutations in any of the CK2 subunits PMID: 27048600
  22. This study shows for the first time that LPS inhibits colonic biotin uptake via decreasing membrane expression of its transporter and that these effects likely involve a CK2-mediated pathway. PMID: 28052864
  23. This study indicates that CKII can modulate the intracellular reactive oxygen species level via FoxO3a. PMID: 27470586
  24. Twelve SNPs from four regions were significantly associated with aggressive disease, among which, three linked SNPs in CSNK1A1 at 5q32 (represented by rs752822) may differentiate GS 4+3 from GS 3+4 patients (OR = 1.44, 95% CI = 1.12-1.87, P = 4.76x10(-3)). PMID: 27515962
  25. using a kinase-inactive mutant of CK2alpha, that RAF-MEK inhibitor resistance did not rely on CK2alpha kinase catalytic function, and both wild-type and kinase-inactive CK2alpha maintained ERK phosphorylation upon inhibition of BRAF or MEK. PMID: 27226552
  26. Inhibition of CK2 increased the expression of metabolic regulators, PDK4 and AMPK along with the key cellular energy sensor CREB. PMID: 27001465
  27. Report provides evidence that CSNK2A1 kinase hyperactivity occurs in vivo in all classes of glioblastomas independently of TP53 status as well as in glial tumors of lower grades and histology. PMID: 27098015
  28. CK2-increased ECE-1c protein stability is related to augmented migration and invasion of colon cancer cells, shedding light on a novel mechanism by which CK2 may promote malignant progression of this disease. PMID: 26543229
  29. This study report increased levels of CK2 in the hippocampus and temporal cortex of AD patients compared to non-demented controls. PMID: 26732432
  30. CK2-mediated phosphorylation of ARC contributed to chemotherapy resistance by inhibiting DOX induced apoptosis; combining DOX with CK2 inhibitor could induce apoptosis of cancer cells synergistically by down-regulating the phosphorylation of ARC PMID: 26172393
  31. Protein kinase CK2 expression predicts relapse survival in ERalpha dependent breast cancer, and modulates ERalpha expression in vitro. PMID: 26703694
  32. Data suggest that complexes of HDAC3-H1.3 with NCOR1 and NCOR2/SMRT accumulate on chromatin in synchronized HeLa cells in late G2 phase and mitosis; deacetylation activity of HDAC3 is activated via phosphorylation of Ser-424 by CK2 only in mitosis. PMID: 26663086
  33. ATG16L1 as a bona fide physiological CSNK2 and PPP1 substrate, which reveals a novel molecular link from CSNK2 to activation of the autophagy-specific ATG12-ATG5-ATG16L1 complex and autophagy induction PMID: 26083323
  34. Data suggest that cryptochromes mediate periodic binding of Ck2b (casein kinase 2beta) to Bmal1 (aryl hydrocarbon receptor nuclear translocator-like protein) and thus inhibit Bmal1-Ser90 phosphorylation by Ck2a (casein kinase 2alpha). [SYNOPSIS] PMID: 26562283
  35. The combination treatment of TRAIL and the CK2 inhibitor decreased p65 nuclear translocation... the treatment of a sub-dose of TRAIL, downregulation of CK2, using both genetic and pharmacological approaches . PMID: 26165401
  36. our findings provide new insights on the potential relevance of the CK2-mediated phosphorylation of B23/NPM in cancer cells, revealing at the same time the potentialities of its pharmacological manipulation for cancer therapy PMID: 25805179
  37. Inhibition of ecto-CK2 by K137-E4 is accompanied by a slower migration of cancer cells as judged by wound healing assays PMID: 26349539
  38. CK2 is widely expressed in follicular, Burkitt and diffuse large B-cell lymphomas and may have role in malignant B-cell growth. PMID: 25788269
  39. High casein kinase II expression is associated with B-cell acute lymphoblastic leukemia. PMID: 26219304
  40. Data show that casein kinase 2 (CK2)-mediated phosphorylation of deubiquitylating enzyme OTUB1 at Ser16 causes nuclear accumulation of OTUB1. PMID: 25872870
  41. Overall results, confirm that a balance of hydrophobic and electrostatic interactions contribute predominantly relative to possible intermolecular halogen/hydrogen bonding ,in binding of halogenated benzotriazoles to the ATP-binding site of hCK2alpha PMID: 25891901
  42. These phosphopeptides include altogether 69 phosphoresidues, a large proportion of which (almost 50%) are generated by CK2, while the others do not conform to the CK2 consensus PMID: 25882195
  43. Over-expressed CK2alpha positively regulate Hh/Gli1 signaling in human mesothelioma. PMID: 25422081
  44. CDK11 and CK2 expression are individually essential for breast cancer cell survival, including TNBC. PMID: 25837326
  45. There is a major role of the CK2alpha-interacting protein CKIP-1 in activation of PAK1 for neoplastic prostate cells transformation. PMID: 26160174
  46. Casein kinase 2-mediated phosphorylation of Hsp90beta and stabilization of PXR is a key mechanism in the regulation of MDR1 expression. PMID: 25995454
  47. Phosphorylation of KCNQ2 and KCNQ3 anchor domains by protein kinase CK2 augments binding to AnkG. PMID: 25998125
  48. Results show that CK2alpha may have an important role in brain tumor-initiating cell maintenance through the regulation of beta-catenin in glioblastoma. PMID: 25241897
  49. CK2-phosphorylation of eIF3j at Ser127 promotes the assembly of the eIF3 complex, a crucial step in the activation of the translation initiation machinery. PMID: 25887626
  50. CK2 phosphorylates and inhibits TAp73 tumor suppressor function to promote expression of cancer stem cell genes and phenotype in head and neck cancer. PMID: 25379016
  51. CagA(+) H. pylori upregulates cellular invasiveness and motility through casein kinase 2. PMID: 25052887
  52. JWA reverses cisplatin resistance via the CK2-XRCC1 pathway in human gastric cancer cells. PMID: 25476899
  53. These findings highlight the potential role of dysregulated miRNA expression regulated by CK2 in breast cancer. PMID: 25120778
  54. Genetic suppression of CK2alpha enhances the apoptosis induced by cisplatin in laryngeal carcinoma cells. PMID: 24831064
  55. CK2 alpha is an independent prognostic indicator for gastric carcinoma patients and is involved in tumorigenesis by regulating the phosphorylation of DBC1. PMID: 24962073
  56. the inhibition of protein kinase CK2 transiently inhibits cell proliferation through the induction of G1 cell cycle arrest and attenuation of protein synthesis by phosphorylating eIF2 alpha. PMID: 24686080
  57. Study revealved that RPL22 binds CK2alpha in lung cancer cells. PMID: 24840952
  58. we characterize CK2 as an essential component of the Jak/STAT pathway PMID: 24742922
  59. Overexpression of CK2 (alpha, alpha' or beta subunits) by transient transfection resulted in decreased Stat3 Ser-727 phosphorylation. PMID: 24726840
  60. The CFTR Ser511 can be phosphorylated by the combined action of tyrosine kinases and CK2 and disclose a new mechanism of hierarchical phosphorylation where the role of the priming kinase is that of removing negative determinants. PMID: 24178769
  61. HCV core up-regulates HAMP gene transcription via a complex signaling network that requires both SMAD/BMP and STAT3 pathways and CK2 involvement PMID: 24718935
  62. protein kinase CK2 has a role in bone marrow stromal cell-fueled multiple myeloma growth and osteoclastogenesis PMID: 24897506
  63. HspA1A also interacts with CK2 and enhances the kinase activities of CK2 during DNA repair. PMID: 23979991
  64. Asymmetric alpha2beta2 tetramers of casein kinase 2 are organized in trimeric rings that correspond to inactive forms of the enzyme. The new crystal structures presented here reveal the symmetric architecture of the isolated active tetramers. PMID: 24175891
  65. These findings identify CK2 as an upstream activating kinase of PAK1, providing a novel mechanism for PAK1 activation. PMID: 23885116
  66. Phosphorylation by CK2 impairs Par-4 proapoptotic functions. PMID: 24457960
  67. Phosphorylation of the CK2 site on SMRT significantly increased affinity for SHARP. PMID: 24268649
  68. miR-125b acts as a tumor suppressor in breast tumorigenesis via its novel direct targets ENPEP, CK2-alpha, CCNJ, and MEGF9. PMID: 24098452
  69. The CSNK2A1 gene has gene dosage gains in glioblastoma, and is significantly associated with the classical glioblastoma subtype. PMID: 24036851
  70. knockdown of CK2alpha expression by siRNA restores the sensitivity of resistant LAMA84 cells to low imatinib concentrations. PMID: 24012109
  71. LSD1 is a novel substrate of protein kinase CK2alpha. PMID: 24486797
  72. crystal structure of CK2alpha with 21 loci of alternative conformations, including a niacin, 19 ethylene glycols and 346 waters, was determined at 1.06 A resolution to an Rwork of 14.0% PMID: 24121351
  73. Protein kinase CK2 alpha is involved in the phosphorylation of the ESCRT-III subunits CHMP3 and CHMP2B, as well as of VPS4B/SKD1, an ATPase that mediates ESCRT-III disassembly. PMID: 24440309
  74. Data suggest that overexpression of CK2a (casein kinase 2, alpha) in liver promotes oncogenesis by mediating EGF/EGFR- (epidermal growth factor/EGF receptor)-induced HDAC2 (histone deacetylase 2) expression in hepatocarcinogenesis. PMID: 24616922
  75. ATF4 is involved in ER stress signalling through the AARE, which further supports our finding that CK2 inhibition provokes an amino acid induced response pathway. PMID: 22609407
  76. These data support a model in which activated Akt enhances rRNA synthesis both by preventing TIF-IA degradation and phosphorylating CK2alpha, which in turn phosphorylates TIF-IA. PMID: 24297901
  77. upregulation of VEGF in primary RPE cells was blocked by a chemical inhibitor of protein kinase CK2 known to suppress induction of ATF4 and VEGF by OxPLs PMID: 24021586
  78. CK2 activity is inhibited by Nopp140 and reactivated by inositol hexakisphosphate by competitive binding at the substrate recognition site of CK2. PMID: 24218616
  79. crystal structure of CK2alpha with a 13-meric cyclic peptide derived from the C-terminal CK2beta segment PMID: 23474121
  80. inhibition of CK2alpha down-regulates Notch1 signalling and subsequently reduces a cancer stem-like cell population in human lung cancer cells PMID: 23651443
  81. CK2alpha' exhibits a striking preference for caspase-3 phosphorylation in cells as compared to CK2alpha and that CK2beta exhibits the capacity to abolish caspase-3 phosphorylation PMID: 23599180
  82. The study identifies casein kinase 2 as an NRF1-binding protein and finds that the knockdown of casein kinase 2 enhances the Nrf1-dependent expression of the proteasome subunit genes. PMID: 23816881
  83. These results identify casein kinase 2 as a new target of PD-1 and reveal an unexpected mechanism by which PD-1 decreases PTEN protein expression while increasing PTEN activity, thereby inhibiting the PI3K/Akt signaling axis. PMID: 23732914
  84. Functional proteomics strategy for validation of protein kinase inhibitors reveals new targets for a TBB-derived inhibitor of protein kinase CK2. PMID: 23017496
  85. Activation of protein kinase CK2 attenuates FOXO3a functioning in a PML-dependent manner. PMID: 23492774
  86. Enzyme inhibition studies and mutational analyses demonstrated that protein kinase CK2-catalyzed phosphorylation of HDAC1 and -2 is crucial for the dissociation of these two enzymes. PMID: 23612983
  87. Data indicate that by altering the scaffold of CK2 inhibitors to obtain selective inhibitors of DYRK1A and PIM1. PMID: 23360763
  88. Studies indicate systematic platforms for studying CK2 inhibitors. PMID: 23416530
  89. These results suggest that Sec31 phosphorylation by CK2 controls the duration of COPII vesicle formation, which regulates ER-to-Golgi trafficking. PMID: 23349870
  90. Phosphorylation of Sec63 by CK2 enhanced its binding to Sec62 PMID: 23287549
  91. PI3K is involved in CKII inhibition-mediated cellular senescence in HCT116 cells. PMID: 23523798
  92. Casein kinase 2alpha inhibition decreases the total protein level of beta-catenin and cell growth. PMID: 23153582
  93. A new crystal structure of the CK2 holoenzyme was determined that plays an essential role in the formation of inactive polymeric assemblies. PMID: 22506723
  94. CK2-mediated hyperphosphorylation of topoisomerase I targets serine 506, enhances topoisomerase I-DNA binding, and increases cellular camptothecin sensitivity. PMID: 23185622
  95. Brd4 association with p53 is modulated by casein kinase II (CK2)-mediated phosphorylation of a conserved acidic region in Brd4 PMID: 23317504
  96. Unbalanced expression of CK2 subunits may drive epithelial-to-mesenchymal transition, thereby contributing to tumour progression. PMID: 22562247
  97. our findings identify CK2 as a novel component of the autophagic machinery and underline the potential of its downregulation to kill glioblastoma cells by overcoming the resistance to multiple anticancer agents. PMID: 23007634
  98. that high intracellular PKCK2 activity confers anoikis resistance on esophageal cancer cells by inducing E- to N-cadherin switching. PMID: 22767590
  99. CK2alpha-NCoR cascade selectively represses the transcription of IP-10 and promotes oncogenic signaling in human esophageal cancer cells. PMID: 22675025
  100. the present results show that miR-186, miR-216b, miR-337-3p, and miR-760 cooperatively promote cellular senescence through the p53-p21(Cip1/WAF1) pathway by CKII downregulation-mediated ROS production in HCT116 cells. PMID: 23137536

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Involvement in disease Okur-Chung neurodevelopmental syndrome (OCNDS)
Subcellular Location Nucleus
Protein Families Protein kinase superfamily, Ser/Thr protein kinase family, CK2 subfamily
Tissue Specificity Expressed in gastric carcinoma tissue and the expression gradually increases with the progression of the carcinoma (at protein level).
Database Links

HGNC: 2457

OMIM: 115440

KEGG: hsa:1457

STRING: 9606.ENSP00000217244

UniGene: Hs.644056

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