Recombinant Human Chondroitin sulfate N-acetylgalactosaminyltransferase 1 (CSGALNACT1), partial

Code CSB-YP006051HU
MSDS
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Source Yeast
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Code CSB-EP006051HU
MSDS
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Source E.coli
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Code CSB-EP006051HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP006051HU
MSDS
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Source Baculovirus
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Code CSB-MP006051HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CSGALNACT1
Uniprot No.
Alternative Names
4-N-acetylgalactosaminyltransferase 1; Beta4GalNAcT 1; beta4GalNAcT; Beta4GalNAcT-1; Beta4GalNAcT1; CGAT1_HUMAN; ChGn; Chondroitin beta-1; Chondroitin beta1 4 N acetylgalactosaminyltransferase; Chondroitin beta1 4 N acetylgalactosaminyltransferase 1; Chondroitin sulfate N acetylgalactosaminyltransferase 1; Chondroitin sulfate N-acetylgalactosaminyltransferase 1; CSGalNAcT 1; CsGalNAcT-1; Csgalnact1; FLJ11264; FLJ13760
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transfers 1,4-N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of glucuronic acid (GlcUA). Required for addition of the first GalNAc to the core tetrasaccharide linker and for elongation of chondroitin chains. Important role in chondroitin chain biosynthesis in cartilage formation and subsequent endochondral ossification. Moreover, is involved in the metabolism of aggrecan.
Gene References into Functions
  1. In men, patients who had multiple sclerosis (MS) with S126L had a slower disease progression. This cSNP might be associated with the sex differences in clinical course of MS. PMID: 26806424
  2. CSGalNAcT-1 and Hapln-1 may play important roles in the pathogenesis of Kashin-Beck disease and osteoarthritis. PMID: 23748413
  3. CSGalNAcT-1 exhibited higher enzymatic efficiency toward monosulfated trisaccharides substituted at C4 or C6 position of Gal1, and at C6 of Gal2 compared with the unsulfated oligosaccharide. PMID: 22156920
  4. CSGALNACT1 missense mutations are associated with neuropathies. PMID: 21160489
  5. higher expression in the fetal skin than in adult skin PMID: 19701759
  6. These results suggest that this enzyme has N-acetylgalactosaminyltransferase activity in both the elongation and initiation of chondroitin sulfate synthesis. PMID: 12163485

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Subcellular Location
Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein.
Protein Families
Chondroitin N-acetylgalactosaminyltransferase family
Tissue Specificity
Ubiquitous, with the highest levels in placenta, thyroid, bladder, prostate and adrenal gland. Detected at low levels in the other tissues examined.
Database Links

HGNC: 24290

OMIM: 616615

KEGG: hsa:55790

STRING: 9606.ENSP00000310891

UniGene: Hs.613729

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