Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Transcription
Alternative Names
CBX7; Chromobox protein homolog 7
Species
Homo sapiens (Human)
Expression Region
1-251aa
Target Protein Sequence
MELSAIGEQVFAVESIRKKRVRKGKVEYLVKWKGWPPKYSTWEPEEHILDPRLVMAYEEKEERDRASGYRKRGPKPKRLLLQRLYSMDLRSSHKAKGKEKLCFSLTCPLGSGSPEGVVKAGAPELVDKGPLVPTLPFPLRKPRKAHKYLRLSRKKFPPRGPNLESHSHRRELFLQEPPAPDVLQAAGEWEPAAQPPEEEADADLAEGPPPWTPALPSSEVTVTDITANSITVTFREAQAAEGFFRDRSGKF
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Amino acids 1-251 form the expressed segment for recombinant Human CBX7. This CBX7 protein is theoretically predicted to have a molecular weight of 32.3 kDa. This CBX7 recombinant protein is manufactured in e.coli. The N-terminal 6xHis tag was fused into the coding gene segment of CBX7, making it easier to detect and purify the CBX7 recombinant protein in the later stages of expression and purification.
Chromobox protein homolog 7 (CBX7) is a protein that regulates gene expression, with one of its most prominent research areas centered around its role in cancer development. The abnormal expression of CBX7 in various cancer types is associated with tumor growth, invasion, and metastasis. Researchers are endeavoring to unveil the molecular mechanisms of CBX7 in the initiation and progression of cancer, aiming to provide new targets for cancer treatment and prevention. Additionally, CBX7's research in stem cells and genomic stability is of great interest. CBX7 is involved in cell fate determination and the self-renewal processes of stem cells, influencing cellular functions under normal physiological conditions. Its regulatory role in the genome involves changes in chromatin structure, making it crucial for understanding cellular development and differentiation processes.