Recombinant Human Chromodomain-helicase-DNA-binding protein 3 (CHD3), partial

Code CSB-YP622515HU
MSDS
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Source Yeast
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Code CSB-EP622515HU
MSDS
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Source E.coli
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Code CSB-EP622515HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP622515HU
MSDS
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Source Baculovirus
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Code CSB-MP622515HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CHD3
Uniprot No.
Alternative Names
226; 2600010P09Rik; AF020312; ATP dependent helicase CHD3; ATP-dependent helicase Chd3; CHD-3; Chd3; CHD3_HUMAN; Chd7; Chromodomain-helicase-DNA-binding protein 3; hZFH; MGC40857; Mi 2 autoantigen 240 kDa protein; Mi 2a; Mi-2 autoantigen 240 kDa protein; Mi2 ALPHA; Mi2-alpha; Prp7; Prp9 1; ZFH; Zinc finger helicase; zinc-finger helicase (Snf2-like)
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Involved in transcriptional repressiobn as part of the NuRD complex. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.
Gene References into Functions
  1. The tandem PHD fingers of CHD3 bind histone H3 tails. PMID: 29020631
  2. CHD3 and CHD4 exhibit distinct nuclear localization patterns in unperturbed cells, revealing a subset of specific target genes. PMID: 28977666
  3. Mutations in CHD3 gene is associated with cutaneous T cell lymphoma and Sezary syndrome. PMID: 26551667
  4. CHD3 was confirmed to interact with NES1 in NS2, and a disruption to this interaction by mutation in NES1 significantly delayed viral vRNPs export and viral propagation. PMID: 25213355
  5. CHD3.1 and ACF1-SNF2H display counteractive activities but similar histone affinities. PMID: 25533843
  6. In this study, the chromatin remodeler chromodomain helicase DNA binding (CHD3) protein is identified as an important component of the initial repression of the herpesvirus genome. PMID: 24425734
  7. effect of progressive C-terminal deletions of CHD3, which indicated that sequences required for repression of PS1 lie between amino acids 1955 and 1877 PMID: 17489097
  8. Pericentrin forms complexes with CHD3 and CHD4, but a distinct CHD3-pericentrin complex is required for centrosomal anchoring of pericentrin/gamma-tubulin and for centrosome integrity. PMID: 17626165
  9. UV radiation regulates Mi-2 through protein translation and stability PMID: 18922793

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Subcellular Location
Nucleus, PML body. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
Protein Families
SNF2/RAD54 helicase family
Tissue Specificity
Widely expressed.
Database Links

HGNC: 1918

OMIM: 602120

KEGG: hsa:1107

STRING: 9606.ENSP00000369716

UniGene: Hs.25601

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