Recombinant Human Coronin-1B (CORO1B)

Code CSB-YP880087HU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP880087HU
MSDS
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Source E.coli
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Code CSB-EP880087HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP880087HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP880087HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CORO1B
Uniprot No.
Alternative Names
COR1B_HUMAN; CORO 1B; CORO1B; Coronin 1B; Coronin 2; Coronin actin binding protein 1B; Coronin-1B; Coronin-2; Coronin1B; Coronin2; DKFZP762I166; OTTHUMP00000236823
Species
Homo sapiens (Human)
Expression Region
1-489
Target Protein Sequence
MSFRKVVRQS KFRHVFGQPV KNDQCYEDIR VSRVTWDSTF CAVNPKFLAV IVEASGGGAF LVLPLSKTGR IDKAYPTVCG HTGPVLDIDW CPHNDEVIAS GSEDCTVMVW QIPENGLTSP LTEPVVVLEG HTKRVGIIAW HPTARNVLLS AGCDNVVLIW NVGTAEELYR LDSLHPDLIY NVSWNHNGSL FCSACKDKSV RIIDPRRGTL VAEREKAHEG ARPMRAIFLA DGKVFTTGFS RMSERQLALW DPENLEEPMA LQELDSSNGA LLPFYDPDTS VVYVCGKGDS SIRYFEITEE PPYIHFLNTF TSKEPQRGMG SMPKRGLEVS KCEIARFYKL HERKCEPIVM TVPRKSDLFQ DDLYPDTAGP EAALEAEEWV SGRDADPILI SLREAYVPSK QRDLKISRRN VLSDSRPAMA PGSSHLGAPA STTTAADATP SGSLARAGEA GKLEEVMQEL RALRALVKEQ GDRICRLEEQ LGRMENGDA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Regulates leading edge dynamics and cell motility in fibroblasts. May be involved in cytokinesis and signal transduction.
Gene References into Functions
  1. VEGF-activated p38alpha phosphorylates coronin 1B at Ser2 and activates the Arp2/3 complex by liberating it from coronin 1B. PMID: 27592029
  2. Coronin 1B constitutively binds to TGF beta receptor I in vascular smooth muscle cells. PMID: 28625921
  3. The loss of junctional NMIIA, upon Coronin 1B knockdown, perturbed RhoA signaling. PMID: 27650961
  4. When cells undergo apoptosis in an epithelial monolayer, coronin 1B is recruited to the junctional cortex at apoptotic/neighbor cell interface in an E-cadherin-dependent fashion to support actin architectural reorganization, contractility, and extrusion. PMID: 27046832
  5. Coronin 1B regulates S1P-induced human lung endothelial cell chemotaxis. PMID: 23667561
  6. analysis of the mechanism for negatively regulating Rho-kinase (ROCK) signaling through Coronin1B protein in neuregulin 1 (NRG-1)-induced tumor cell motility PMID: 22563075
  7. Coronin 1B regulates leading edge dynamics and controls cell motility and interactions with the actin-related protein (Arp)2/3 complex PMID: 16027158
  8. Study concludes that Coronin 1B's coordination of filament formation by Arp2/3 complex and filament turnover by Cofilin is required for effective lamellipodial protrusion and cell migration. PMID: 17350576
  9. In rabbits, cholinergic stimulation increases the phosphorylation of coronin 1B in gastric mucosal cells PMID: 9915840

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Subcellular Location
Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, stress fiber.
Protein Families
WD repeat coronin family
Database Links

HGNC: 2253

OMIM: 609849

KEGG: hsa:57175

STRING: 9606.ENSP00000340211

UniGene: Hs.6191

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