Product Details

Purity

>85% (SDS-PAGE)

Target Names

CDK14

Uniprot No.

O94921

Alternative Names

cdk14; CDK14_HUMAN; Cell division protein kinase 14; Cyclin-dependent kinase 14; hPFTAIRE1; KIAA0834; mKIAA0834; PFTAIRE protein kinase 1; PFTAIRE1; PFTK1; Serine/threonine protein kinase PFTAIRE 1; Serine/threonine-protein kinase PFTAIRE-1

Species

Homo sapiens (Human)

Expression Region

1-469

Target Protein Sequence

MCDLIEPQPA EKIGKMKKLR RTLSESFSRI ALKKDDTTFD EICVTKMSTR NCQGMDSVIK PLDTIPEDKK VRVQRTQSTF DPFEKPANQV KRVHSENNAC INFKTSSTGK ESPKVRRHSS PSSPTSPKFG KADSYEKLEK LGEGSYATVY KGKSKVNGKL VALKVIRLQE EEGTPFTAIR EASLLKGLKH ANIVLLHDII HTKETLTLVF EYVHTDLCQY MDKHPGGLHP DNVKLFLFQL LRGLSYIHQR YILHRDLKPQ NLLISDTGEL KLADFGLARA KSVPSHTYSN EVVTLWYRPP DVLLGSTEYS TCLDMWGVGC IFVEMIQGVA AFPGMKDIQD QLERIFLVLG TPNEDTWPGV HSLPHFKPER FTLYSSKNLR QAWNKLSYVN HAEDLASKLL QCSPKNRLSA QAALSHEYFS DLPPRLWELT DMSSIFTVPN VRLQPEAGES MRAFGKNNSY GKSLSNSKH

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Serine/threonine-protein kinase involved in the control of the eukaryotic cell cycle, whose activity is controlled by an associated cyclin. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by mediating the phosphorylation of LRP6 at 'Ser-1490', leading to the activation of the Wnt signaling pathway. Acts as a regulator of cell cycle progression and cell proliferation via its interaction with CCDN3. Phosphorylates RB1 in vitro, however the relevance of such result remains to be confirmed in vivo. May also play a role in meiosis, neuron differentiation and may indirectly act as a negative regulator of insulin-responsive glucose transport.

Gene References into Functions

CDK14 was validated to be the target protein the miR-139. PMID: 29944887
OIP5-AS1 acts as a miR-223 'sponge' to trigger CDK14 expression to promote osteosarcoma tumorigenesis. PMID: 30119217
knockdown of E2F5 and PFTK1 mimicked the tumor-suppressive effects of miR-1-3p overexpression on PCa progression. Conversely, concomitant knockdown of miR-1-3p and E2F5 and PFTK1 substantially reversed the inhibitory effects of either E2F5 or PFTK1 silencing alone. PMID: 30185212
MiR-613 and CDK14 may serve as potential therapeutic targets for the treatment of glioma. PMID: 29289838
CDK14 mediated miR-1202 to exert its anti-tumor effects. PMID: 29217161
CDK14 expression was closely associated with poor prognosis and overall survival of Osteosarcoma patients. miR-216a inhibits CDK14 expression by binding to the 3'-untranslated region of CDK14. PMID: 29022909
PFTK1 expression is significantly higher in CRC tissues compared with matched adjacent noncancerous colorectal tissues. The associations between PFTK1 expression and clinicopathological characters, and PFTK1 expression and the OS rate following resection suggested that PFTK1 may represent a novel biomarker of poor prognosis in CRC PMID: 28498444
Results show that CDK14 and DYRK2 expression were inversely correlated in human breast cancer tissues and identified CDK14 as a target of DYRK2. PMID: 29193658
miR-455 inhibits breast cancer cell proliferation through targeting CDK14 PMID: 28300591
findings revealed an unrecognized role of Caprin-2 in facilitating LRP5/6 constitutive phosphorylation at G2/M through forming a quaternary complex with CDK14, Cyclin Y, and LRP5/6. PMID: 27821587
these results suggest that knockdown of PFTK1 inhibited the proliferation and invasion of colon cancer cells as well as the EMT progress by suppressing the Sonic hedgehog signaling pathway. PMID: 27458094
we report that PFTK1 downregulation might inhibit the proliferation and invasion of NSCLC cells by suppressing the Wnt/beta-catenin signaling pathway. PMID: 27458099
The expression level of PFTK1 was correlated with tumor grade, FIGO stage, lymph node metastasis of EOC patients as well as poor prognosis. PMID: 26772918
Knockdown of PFTK1 inhibited the proliferation and invasion of pancreatic cancer cells as well as the EMT progress by suppressing the PI3K/Akt signaling pathway. PMID: 26823712
PFTK1 overexpression promoted proliferation, migration and invasion of gastric cancer cells, while PFTK1 knockdown led to the opposite results. PMID: 26488471
Knockdown of PFTK1 increases E-cadherin expression, decreases vimentin expression and inhibits migration of glioma cells. PMID: 26234562
Up-regulated PFTK1 might promote breast cancer progression. PMID: 26033031
Cigarette smoke down regulates CDK14 levels and impairs Wnt signaling. PMID: 25680692
findings indicate that brain PP-1I associates with and is regulated by the associated protein kinases C-TAK1 and PFTK1 PMID: 25028520
binding of CCNY to 14-3-3 significantly enhanced the association between CCNY and CDK14 PMID: 24618387
Human cyclin Y (CCNY) is a phosphoprotein in vivo and phosphorylation of CCNY by CDK14 triggers its ubiquitination and degradation. PMID: 24794231
in patients with oesophageal squamous cell carcinoma 5-year overall survival rate was poorer in patients positive for PFTK1 than those with negative expression; uni- and multivariate analyses identified PFTK1 as an independent marker of prognosis PMID: 22333595
Taken together, our data propose a novel, oncogene-tumor suppressor interplay, where oncogenic PFTK1 confers HCC cell motility through inactivating the actin-binding motile suppressing function of TAGLN2 via phosphorylation. PMID: 21577206
Novel biological cascade that involved the phosphorylation activation of CaD by PFTK1 kinase in promoting formation of actin stress fibers. PMID: 21184254
Ser119 is crucial for the interaction between hPFTAIRE1 and the 14-3-3 proteins. Binding with the 14-3-3 proteins does not contribute to the subcellular localization of the hPFTAIRE1, although the binding may be involved in its signaling regulation. PMID: 16775625
PFTK1 acts as a cyclin-dependent kinase that regulates cell cycle progression and cell proliferation PMID: 17517622

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Subcellular Location

Cell membrane; Peripheral membrane protein. Cytoplasm. Nucleus. Note=Recruited to the cell membrane by CCNY.

Protein Families

Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily

Tissue Specificity

Highly expressed in brain, pancreas, kidney, heart, testis and ovary. Also detected at lower levels in other tissues except in spleen and thymus where expression is barely detected.

Database Links

HGNC: 8883

OMIM: 610679

KEGG: hsa:5218

STRING: 9606.ENSP00000265741

UniGene: Hs.258576

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Recombinant Human Cyclin-dependent kinase 14 (CDK14)

Product Details

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Target Background

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