Purity
Greater than 85% as determined by SDS-PAGE.
Species
Homo sapiens (Human)
Target Protein Sequence
IPGGLSEAKPATPEIQEIVDKVKPQLEEKTNETYGKLEAVQYKTQVVAGTNYYIKVRAGDNKYMHLKVFKSLPGQNEDLVLTGYQVDKNKDDELTGF
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal GST-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The recombinant Human CSTA was expressed with the amino acid range of 2-98. The expected molecular weight for the CSTA protein is calculated to be 38.3 kDa. This CSTA protein is produced using e.coli expression system. The CSTA coding gene included the N-terminal GST tag, which simplifies the detection and purification processes of the recombinant CSTA protein in following stages of expression and purification.
Human cystatin-A (CSTA), also known as stefin A, is a member of the cystatin superfamily of cysteine proteinase inhibitors. CSTA plays a crucial role in regulating the activity of cysteine proteases by forming tight, reversible complexes with them. CSTA is primarily expressed in the epidermis, where it helps control the activity of proteases involved in skin homeostasis. Its inhibition of cathepsins protects against excessive protein degradation, contributing to the maintenance of skin integrity. Beyond its role in skin physiology, CSTA has been implicated in various pathological conditions, including cancer, where its dysregulation may influence tumor progression. Understanding CSTA's functions is vital for unraveling its diverse roles in health and disease.
