Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

COX5A

Uniprot No.

P20674

Research Area

Metabolism

Alternative Names

COX; COX VA; COX5A; COX5A_HUMAN; Cytochrome c oxidase polypeptide Va; Cytochrome c oxidase polypeptide; mitochondrial; Cytochrome c oxidase subunit 5A; Cytochrome c oxidase subunit 5A; mitochondrial; Cytochrome c oxidase subunit Va; mitochondrial; Mitochondrial cytochrome c oxidase subunit Va; VA

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

42-150aa

Target Protein Sequence

SHGSQETDEEFDARWVTYFNKPDIDAWELRKGINTLVTYDMVPEPKIIDAALRACRRLNDFASTVRILEVVKDKAGPHKEIYPYVIQELRPTLNELGISTPEELGLDKV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

39.5kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal GST-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The region for expressing recombinant Human COX5A contains amino acids 42-150. The calculated molecular weight for this COX5A protein is 39.5 kDa. Expression of this COX5A protein is conducted in e.coli. The N-terminal GST tag was fused into the coding gene segment of COX5A, making it easier to detect and purify the COX5A recombinant protein in the later stages of expression and purification.

The human cytochrome c oxidase subunit 5A, mitochondrial (COX5A), is a crucial component of cytochrome c oxidase (complex IV) in the mitochondrial respiratory chain. COX5A is responsible for facilitating the transfer of electrons during oxidative phosphorylation. COX5A plays a pivotal role in the final step of the electron transport chain, where electrons are transferred to molecular oxygen, producing water. This process is essential for ATP synthesis and cellular energy production. Research on COX5A focuses on understanding its role in mitochondrial function, energy metabolism, and its implications in various diseases related to mitochondrial dysfunction.

Target Background

Function

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

Gene References into Functions

The monomeric COX1 assembly intermediate accumulates demonstrating a function of COX5A in complex IV biogenesis. A potential therapeutic lead is demonstrated by showing that copper supplementation leads to partial rescue of complex IV deficiency in patient fibroblasts. PMID: 28247525
Our current study showed that COX Va may play a role in migration and invasion of NSCLC cells and can be used as a biomarker to predict aggressiveness of NSCLC. PMID: 22748147
Novel insights into the assembly and function of human nuclear-encoded cytochrome c oxidase subunits 5a PMID: 20307258
Data show that Bcl-2 expression positively influences the targeting of nuclear-encoded COX Va and Vb to the mitochondria of cancer cells. PMID: 19834492
study points to a role for surfeit 1(SURF1) in promoting the association of cytochrome c oxidase II with the cytochrome c oxidase I.cytochrome c oxidase subunit 4.cytochrome c oxidase subunit 5A subassembly PMID: 14607829

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Involvement in disease

Mitochondrial complex IV deficiency is a rare condition caused by mutation in COX5A that lead to pulmonary arterial hypertension (PAH), failure to thrive and lactic acidemia.

Subcellular Location

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.

Protein Families

Cytochrome c oxidase subunit 5A family

Database Links

HGNC: 2267

OMIM: 603773

KEGG: hsa:9377

STRING: 9606.ENSP00000317780

UniGene: Hs.401903

Code	CSB-RP004144h
Abbreviation	Recombinant Human COX5A protein
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Cytochrome c oxidase subunit 5A, mitochondrial (COX5A)

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