Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

DENND1A

Uniprot No.

Q8TEH3

Research Area

Neuroscience

Alternative Names

Connecdenn 1; Connecdenn; DEN1A_HUMAN; DENN domain-containing protein 1A; DENN/MADD domain containing 1A; dennd1a; FAM31A; FLJ21129; FLJ38464; KIAA1608; OTTHUMP00000022090; Protein FAM31A; RP11 230L22.3

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-559aa

Target Protein Sequence

MGSRIKQNPETTFEVYVEVAYPRTGGTLSDPEVQRQFPEDYSDQEVLQTLTKFCFPFYVDSLTVSQVGQNFTFVLTDIDSKQRFGFCRLSSGAKSCFCILSYLPWFEVFYKLLNILADYTTKRQENQWNELLETLHKLPIPDPGVSVHLSVHSYFTVPDTRELPSIPENRNLTEYFVAVDVNNMLHLYASMLYERRILIICSKLSTLTACIHGSAAMLYPMYWQHVYIPVLPPHLLDYCCAPMPYLIGIHLSLMEKVRNMALDDVVILNVDTNTLETPFDDLQSLPNDVISSLKNRLKKVSTTTGDGVARAFLKAQAAFFGSYRNALKIEPEEPITFCEEAFVSHYRSGAMRQFLQNATQLQLFKQFIDGRLDLLNSGEGFSDVFEEEINMGEYAGSDKLYHQWLSTVRKGSGAILNTVKTKANPAMKTVYKFAKDHAKMGIKEVKNRLKQKDIAENGCAPTPEEQLPKTAPSPLVEAKDPKLREDRRPITVHFGQVRPPRPHVVKRPKSNIAVEGRRTSVPSPEQNTIATPATLHILQKSITHFAAKFPTRGWTSSSH
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

79.4kDa

Protein Length

Full Length of Isoform 2

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 1-559 form the expressed segment for recombinant Human DENND1A. The calculated molecular weight for this DENND1A protein is 79.4 kDa. This DENND1A protein is produced using e.coli expression system. Fusion of the N-terminal 6xHis-SUMO tag into the DENND1A encoding gene fragment was conducted, allowing for easier detection and purification of the DENND1A protein in subsequent stages.

Human DENN domain-containing protein 1A (DENND1A) primarily functions as a guanine nucleotide exchange factor (GEF) for Rab GTPases, playing a pivotal role in regulating intracellular membrane trafficking and vesicle transport. In cellular and molecular investigations, the study of DENND1A provides crucial insights into the dynamic processes of cellular organelle trafficking and vesicular formation. DENND1A's involvement in insulin granule trafficking connects it with diabetes research, while its association with cancer implicates it in cell migration and invasion. Exploring DENND1A opens avenues for understanding membrane dynamics, cellular signaling, and the pathophysiology of diseases related to vesicular trafficking, offering potential applications in diabetes and cancer research.

Target Background

Function

Guanine nucleotide exchange factor (GEF) regulating clathrin-mediated endocytosis through RAB35 activation. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB35 into its active GTP-bound form. Regulates clathrin-mediated endocytosis of synaptic vesicles and mediates exit from early endosomes. Binds phosphatidylinositol-phosphates (PtdInsPs), with some preference for PtdIns(3)P.

Gene References into Functions

The association of DENND1A rs10818854 and rs10986105 variants with PCOS in Tunisian but not Bahraini women confirms the dependence of this association on the ethnic/racial origin of study subjects. PMID: 29325736
Genome-wide association studies have identified DENND1A as a candidate gene for polycystic ovary syndrome (PCOS). PCOS theca cells are shown to overexpress DENND1A splice variant 2 (V2), but not V1. Also, the genetic variation 330 bp upstream of exon 20A is not associated with VE variant expression. PMID: 27297658
DENND1A gene variant is likely to have influence on PCOS risk. PMID: 27488699
This meta-analysis suggested that rs2479106 and rs10818854 polymorphisms in the DENND1A gene were associated with increased risk of polycystic ovary syndrome PMID: 26757598
The guanine nucleotide exchange factor activity of connecdenn 1 and 2 are autoinhibited, and this is regulated by Akt activation downstream of insulin stimulation. PMID: 26055712
We demonstrated lack of association of DENND1A SNPs rs10818854, rs2479106, and rs10986105, previously associated with PCOS in Asians, with PCOS in Bahraini Arab women. PMID: 25626177
sequence analysis of the DENND1A gene of patients with PCOS did not identify alterations that alone could be responsible for the PCOS pathogenesis PMID: 24086769
DENND1A, plays a key role in the hyperandrogenemia associated with polycystic ovary syndrome. PMID: 24706793
THADA and DENND1A, carry risk alleles that are associated with endocrine and metabolic disturbances in PCOS patients of Han Chinese descent. PMID: 23208300
Our results suggest that SNPs rs2479106 in gene DENND1A and rs13405728 in gene LHCGR are associated with endometrioid adenocarcinoma. PMID: 22902918
The rs2479106 G allele was associated with decreased PCOS susceptibility PMID: 22504079
Variants in DENND1A are associated with polycystic ovary syndrome in women of European ancestry. PMID: 22547425
At least two of the PCOS susceptibility loci identified in the Chinese PCOS GWAS (DENND1A and THADA) are also associated with PCOS in European derived populations. PMID: 22180642

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Involvement in disease

Genetic variants in DENND1A may play a role in susceptibility to polycystic ovary syndrome (PCOS), the most common endocrine disease among premenopausal women. PCOS is a complex disorder characterized by infertility, hirsutism, obesity, various menstrual disturbances, and enlarged ovaries studded with atretic follicles.

Subcellular Location

Cytoplasmic vesicle, clathrin-coated vesicle membrane; Peripheral membrane protein. Cell junction, synapse, presynaptic cell membrane.

Database Links

HGNC: 29324

OMIM: 613633

KEGG: hsa:57706

STRING: 9606.ENSP00000362727

UniGene: Hs.744995

Code	CSB-EP823472HU
Abbreviation	Recombinant Human DENND1A protein
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human DENN domain-containing protein 1A (DENND1A)

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