Recombinant Human DEP domain-containing protein 1A (DEPDC1), partial

Code CSB-YP722576HU
MSDS
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Source Yeast
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Code CSB-EP722576HU
MSDS
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Source E.coli
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Code CSB-EP722576HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP722576HU
MSDS
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Source Baculovirus
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Code CSB-MP722576HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DEPDC1
Uniprot No.
Alternative Names
5830484J08Rik; Cell cycle control protein SDP35; DEP domain containing 1; DEP domain containing protein 1A; DEP domain-containing protein 1A; DEP.8; DEP1A_HUMAN; DEPDC 1; DEPDC 1A; DEPDC1; DEPDC1-V2; Depdc1a; FLJ20354; SDP 35; SDP35
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May be involved in transcriptional regulation as a transcriptional corepressor. The DEPDC1A-ZNF224 complex may play a critical role in bladder carcinogenesis by repressing the transcription of the A20 gene, leading to transport of NF-KB protein into the nucleus, resulting in suppression of apoptosis of bladder cancer cells.
Gene References into Functions
  1. Our results demonstrated that increased expression of SDP35/DEPDC1A and XPT1/DEPDC1B correlates with metastatic progression of metastatic soft tissue sarcoma PMID: 29297565
  2. The results of this study suggest that DEPDC1 represents a target molecule for the treatment of glioma. PMID: 28555424
  3. DEPDC1a, but not DEPDC1b, was required for the integrity of centrosome and organization of the bipolar spindle PMID: 28602627
  4. DEPDC1 mRNA and protein expression levels were dramatically increased in prostate cancer tissues and cell lines. Overexpression of DEPDC1 promoted, but depletion of DEPDC1 inhibited cell proliferation by regulating the G1-S phase cell cycle transition. PMID: 28634077
  5. miR-130a is an epigenetically regulated miRNA involved in regulation of key molecular and phenotypic features of prostate carcinogenesis, acting as a tumour suppressor miRNA by targeting SEC23B and DEPDC1. PMID: 27984115
  6. Results suggest that protocadherin 10 (PCDH10)-Dishevelled, EGL-10 and Pleckstrin domain containing 1 (DEPDC1)-caspase signaling may be a novel regulatory axis in endometrial endometrioid carcinoma (EEC) development. PMID: 26970279
  7. DEPDC1, plays a pivotal role in the regulation of proper mitotic progression. PMID: 25902835
  8. High DEPDC1 mRNA expression is associated with hepatocellular carcinoma/ PMID: 25605201
  9. DEPDC1 regulates vincristine-induced cell death by promoting JNK-dependent degradation of the BCL-2 family protein MCL1. PMID: 25064737
  10. Inhibition of DEPDC1A, a bad prognostic marker in multiple myeloma, delays growth and induces mature plasma cell markers in malignant plasma cells. PMID: 23646139
  11. Coimmunoprecipitation and immunocytochemistry revealed that DEPDC1 interacted and colocalized with zinc finger transcription factor ZNF224, a known transcriptional repressor. PMID: 20587513

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Subcellular Location
Nucleus. Note=Colocalizes with ZNF224 at the nucleus.
Tissue Specificity
Expressed in testis. Up-regulated in bladder cancer cells (at protein level).
Database Links

HGNC: 22949

OMIM: 612002

KEGG: hsa:55635

STRING: 9606.ENSP00000412292

UniGene: Hs.445098

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