Recombinant Human DNA-3-methyladenine glycosylase(MPG),partial

Code CSB-YP014749HU
Product Type Recombinant Protein
Size US$2010
Uniprot No. P29372
Relevance Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Storage Buffer Tris-based buffer,50% glycerol
Alias 3-alkyladenine DNA glycosylase3-methyladenine DNA glycosidaseADPGN-methylpurine-DNA glycosylase
Species Homo sapiens (Human)
Purity Greater than 90% as determined by SDS-PAGE.
Sequence QFCRRMGQKKQRPARAGQPHSSSDAAQAPAEQPHSSSDAAQAPCPRERCLGPPTTPGPYRSIYFSSPKGHLTRLGLEFFDQPAVPLARAFLGQVLVRRLPNGTELRGRIVETEAYLGPEDEAAHSRGGRQTPRNRGMFMKPGTLYVYIIYGMYFCMNISSQGDGACVLLRALEPLEGLETMRQLRSTLRKGTASRVLKDRELCSGPSKLCQALAINKSFDQRDLAQDEAVWLERGPLEPSEPAVVAAARVGVGHAGEWARKPLRFYVRGSPWVSVVDRVAEQDTQA
Research Area Epigenetics and Nuclear Signaling
Source Yeast
Gene Names MPG
Protein Names Recommended name: DNA-3-methyladenine glycosylase EC= 3.2.2.21 Alternative name(s): 3-alkyladenine DNA glycosylase 3-methyladenine DNA glycosidase ADPG N-methylpurine-DNA glycosylase
Expression Region 13-298aa
Tag Info N-terminal 6xHis-tagged
Mol. Weight 33.5kDa
Protein Description Partial
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Function Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.
Subcellular Location Cytoplasm, Mitochondrion matrix, mitochondrion nucleoid, Nucleus
Protein Families DNA glycosylase MPG family
Database Links

HGNC: 7211

OMIM: 156565

KEGG: hsa:4350

STRING: 9606.ENSP00000219431

UniGene: Hs.459596

Pathway DNA repair pathway

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