Recombinant Human DNA polymerase delta subunit 3 (POLD3)

1. findings demonstrate a key role of POLD1 and POLD3 in genome stability and S-phase progression revealing RNA-DNA hybrids-dependent effects for POLD3 that might be partly due to its Pol zeta interaction. PMID: 27974823
2. The proofreading activity of DNA polymerase delta plays a role in shunting DNA mismatch repair to an EXO1-dependent excision pathway as opposed to directly participating in gap formation via its 3'-5' exonuclease activity. PMID: 28934474
3. The mitotic DNA synthesis is RAD52 dependent, and RAD52 is required for the timely recruitment of MUS81 and POLD3 to common fragile sites in early mitosis. PMID: 27984745
4. data support Poldelta contributing to translesion synthesis in vivo and suggest that the mutagenesis resulting from loss of Poldelta proofreading activity may in part be explained by enhanced lesion bypass PMID: 27185888
5. Studies indicate that DNA polymerase delta 3 (Pol delta3) exhibits significant differences in properties with a major impact on cellular processes in genomic surveillance, DNA replication and DNA repair. PMID: 26916162
6. Data suggest that relatively high affinity binding of PolD3-RIR motif to Rev1-C-terminal domain displaces subunits from PolN, Pol-iota, or PolK from Rev1 complex and promotes formation of Rev1/PolZ4 assembly with PCNA for translesion DNA replication. PMID: 26982350
7. POLD3 is SUMOylated by SUMO2 in response to replication stress and could play an important role in the regulation of cellular response to DNA replication stress. PMID: 25497329
8. Findings provide evidence for the novel concept that Pol delta3 has a role in lagging strand synthesis, and that both forms of Pol delta3 and 4 may participate in DNA replication in higher eukaryotic cells. PMID: 24035200
9. In a cyclin E overexpression model of DNA replication stress, POLD3, the human ortholog of POL32, was required for cell cycle progression and processive DNA synthesis. PMID: 24310611
10. Pol delta3 is the predominant form of Pol delta at sites of UV damage as a result of p12 degradation; results show that Pol delta at the DNA damage site is the Pol delta trimer lacking p12 regardless of the cell cycle phase PMID: 22801543
11. We identified three new CRC risk loci at 6p21 (rs1321311, near CDKN1A; P = 1.14 x 10(-10)), 11q13.4 (rs3824999, intronic to POLD3; P = 3.65 x 10(-10)) and Xp22.2 (rs5934683, near SHROOM2; P = 7.30 x 10(-10)). PMID: 22634755
12. Serine-458, located in the proliferating cell nuclear antigen (PCNA)-interacting motif of Poldelta(p68), is a phosphorylation site for protein kinase A (PKA); p68 mutation results in decreased p68 affinity for PCNA as well as processivity of Poldelta. PMID: 22148433
13. show that there is a direct interaction between p66 and PCNA in living cells during DNA replication. The dominant negative effect upon growth resulting from expression of p66 sub-domains confirms that the p66-PCNA interaction is essential in vivo. PMID: 16000169
14. POLD3, plays a crucial role in the efficient recycling of PCNA during dissociation-association cycles of pol delta during elongation phase of DNA replication. PMID: 17932049
15. Taken together, the results provide evidence that concurrent phosphorylation events in p66 may positively and negatively regulate its activity and interactions with other components of the replisome during the cell cycle. PMID: 18157942
16. As a first step towards understanding the functional importance of their regulatory subunit interactions, the three-dimensional structure of the p50-p66 heterodimer of human Pol delta has been solved using X-ray crystallography. PMID: 18765914
17. the crystal structure of p50*p66(N) complex featuring oligonucleotide binding and phosphodiesterase domains in winged helix-turn-helix N-terminal domain in p66 PMID: 18818516

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HGNC: 20932

OMIM: 611415

KEGG: hsa:10714

STRING: 9606.ENSP00000263681

UniGene: Hs.82502