Recombinant Human DNA polymerase nu (POLN), partial

Product Details

Purity

≥85% (SDS-PAGE)

Target Names

POLN

Uniprot No.

Q7Z5Q5

Alternative Names

POLNDNA polymerase nu; EC 2.7.7.7

Species

Homo sapiens (Human)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

DNA polymerase with very low fidelity that catalyzes considerable misincorporation by inserting dTTP opposite a G template, and dGTP opposite a T template. Is the least accurate of the DNA polymerase A family (i.e. POLG, POLN and POLQ). Can perform accurate translesion DNA synthesis (TLS) past a 5S-thymine glycol. Can perform efficient strand displacement past a nick or a gap and gives rise to an amount of product similar to that on non-damaged template. Has no exonuclease activity. Error-prone DNA polymerase that preferentially misincorporates dT regardless of template sequence. May play a role in TLS during interstrand cross-link (ICL) repair. May be involved in TLS when genomic replication is blocked by extremely large major groove DNA lesions. May function in the bypass of some DNA-protein and DNA-DNA cross-links. May have a role in cellular tolerance to DNA cross-linking agents. Involved in the repair of DNA cross-links and double-strand break (DSB) resistance. Participates in FANCD2-mediated repair. Forms a complex with HELQ helicase that participates in homologous recombination (HR) repair and is essential for cellular protection against DNA cross-links.

Gene References into Functions

Data suggest that translesion DNA synthesis mediated by (1) POLI-dependent pathway (2) REV1- and POLN-dependent pathway, or (3) POLtheta-dependent pathway occur in predominantly error-free manner in human cells. (POLI = DNA polymerase iota; REV1 = DNA repair protein-REV1; POLN = DNA polymerase nu; POLtheta = DNA polymerase theta) PMID: 29330301
The structural data are consistent with the observed tendency of hpol eta to insert both dC and dT opposite the O(6)-MeG lesion with similar efficiencies. PMID: 27694439
Five x-ray crystal structures of hpol eta ternary complexes were determined, three at the insertion and two at the extension stage. PMID: 27226627
The Proliferating Cell Nuclear Antigen (PCNA)-interacting Protein (PIP) Motif of DNA Polymerase eta Mediates Its Interaction with the C-terminal Domain of Rev1. PMID: 26903512
This is the first crystal structure of a DNA polymerase with an incoming rNTP opposite a DNA lesion. PMID: 26740629
Pol nu catalyzes both correct and mispair formation with high catalytic efficiency. PMID: 25963146
WRN improves the efficiency and fidelity of hpol eta to promote more effective replication of DNA. PMID: 23045531
Epstein-Barr virus BPLF1 deubiquitinates PCNA and attenuates polymerase eta recruitment to DNA damage sites PMID: 22623772
Our finding suggests that polymorphisms in DNA repair genes, POLN and PRKDC, were associated with increased melanoma risk in melanoma families with and without CDKN2A mutations. PMID: 21671477
both yeast and human pol eta synthesize past the 3'-(m)C CPD in a >99% error-free manner, consistent with the highly water-exposed nature of the active site. PMID: 22262850
The lower fidelity of POLN compared to that of Klenow fragment of Escherichia coli can be attributed to a much lower catalytic efficiency for correct dNTP incorporation, whereas both enzymes have similar kinetic parameters for G-dTTP misinsertion. PMID: 22008035
Described is a novel translesion DNA synthesis substrate specificity of pol nu, demonstrating that it is able to bypass exceptionally large DNA lesions whose linkages are through the DNA major groove. PMID: 20102227
Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20379614
The recently discovered polymerase POLN identified here is involved in repair of DNA cross-links. PMID: 19995904
The DNA cross-linking agent mitomycin C hypersensitivity of PolN knockdown cells is rescued by overexpression of DNA polymerase-proficient PolN but not by DNA polymerase-deficient PolN. PMID: 19908865
identification as homolog to Mus308 PMID: 12794064
pol nu error rates for all 12 single base-base mismatches and for insertion and deletion errors PMID: 17118716

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Subcellular Location

Nucleus.

Protein Families

DNA polymerase type-A family

Tissue Specificity

Highly expressed in testis and heart. Weakly expressed in skeletal muscle.

Database Links

HGNC: 18870

OMIM: 610887

KEGG: hsa:353497

STRING: 9606.ENSP00000372316

UniGene: Hs.368454

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Recombinant Human DNA polymerase nu (POLN), partial

Product Details

Customer Reviews and Q&A

Target Background

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