This POLQ protein is a semi-custom product. There are 5 expression system options: Yeast, E. coli, In Vivo Biotinylation in E. coli, Baculovirus, and Mammalian cell. Your requirements will be given top priority in determining the protein tags. For proteins within 800 aa, risk-free custom service is guaranteed. It means you will not be charged if the protein cannot be delivered.
POLQ is a DNA polymerase that plays a crucial role in various DNA repair processes such as translesion DNA synthesis (TLS) and DNA double-strand break (DSB) repair [1]. POLQ seals post-replicative single-stranded DNA gaps to maintain genome stability in BRCA-deficient cancer cells [2]. Additionally, POLQ participates in repairing end joining of DNA breaks during processes like immunoglobulin class-switching, resulting in insertions of base pairs at the joins with homology to specific DNA sequences [3]. Furthermore, POLQ is involved in repairing CRISPR/Cas9-induced double-stranded breaks, highlighting its role in DNA repair mechanisms [4]. POLQ has been identified as synthetic lethal with several DNA repair genes, including BRCA1/2, potentially for cancer target therapy, particularly in homologous recombination-deficient cancers like BRCA-deficient tumors [5][6].
References:
[1] K. Shinmura, H. Kato, Y. Kawanishi, K. Yoshimura, K. Tsuchiya, Y. Takaharaet al., Polq overexpression is associated with an increased somatic mutation load and plk4 overexpression in lung adenocarcinoma, Cancers, vol. 11, no. 5, p. 722, 2019. https://doi.org/10.3390/cancers11050722
[2] O. Beláň, M. Sebald, M. Adamowicz, R. Anand, A. Vančevska, J. Neveset al., Polq seals post-replicative ssdna gaps to maintain genome stability in brca-deficient cancer cells, Molecular Cell, vol. 82, no. 24, p. 4664-4680.e9, 2022. https://doi.org/10.1016/j.molcel.2022.11.008
[3] M. Yousefzadeh, D. Wyatt, K. Takata, Y. Mu, S. Hensley, J. Tomidaet al., Mechanism of suppression of chromosomal instability by dna polymerase polq, Plos Genetics, vol. 10, no. 10, p. e1004654, 2014. https://doi.org/10.1371/journal.pgen.1004654
[4] K. Mara, F. Charlot, A. Guyon‐Debast, D. Schaefer, C. Collonnier, M. Grelonet al., polq plays a key role in the repair of crispr/cas9‐induced double‐stranded breaks in the moss physcomitrella patens, New Phytologist, vol. 222, no. 3, p. 1380-1391, 2019. https://doi.org/10.1111/nph.15680
[5] A. Schrempf, J. Slyšková, & J. Loizou, Targeting the dna repair enzyme polymerase θ in cancer therapy, Trends in Cancer, vol. 7, no. 2, p. 98-111, 2021. https://doi.org/10.1016/j.trecan.2020.09.007
[6] Z. Qin, Molecular generation, qsar, and molecular dynamic simulation studies for virtual screening of dna polymerase theta inhibitors, Current Computer - Aided Drug Design, vol. 20, 2024. https://doi.org/10.2174/011573409930514224050805183
