Purity
Greater than 85% as determined by SDS-PAGE.
Research Area
Epigenetics and Nuclear Signaling
Species
Homo sapiens (Human)
Expression Region
321-554aa
Target Protein Sequence
GDEDHVVSLCYETICDNHSVLLFCPSKKWCEKLADIIAREFYNLHHQAEGLVKPSECPPVILEQKELLEVMDQLRRLPSGLDSVLQKTVPWGVAFHHAGLTFEERDIIEGAFRQGLIRVLAATSTLSSGVNLPARRVIIRTPIFGGRPLDILTYKQMVGRAGRKGVDTVGESILICKNSEKSKGIALLQGSLKPVRSCLQRREGEEVTGSMIRAILEIIVGGVASTSQDMHTYA
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
To produce recombinant Human POLQ protein, a well-established recombinant DNA technology is the key. A DNA template of POLQ was constructed with N-terminal 10xHis tag & C-terminal Myc tag using the technique. Once the template was made, the recombinant Human POLQ protein could be produced with it efficiently. CUSABIO has built a strict QC system to ensure quality. The expression region is 321-554aa of the Human POLQ. The purity of this recombinant is 85% determined by SDS-PAGE.
POLQ (POLH) is a protein coding gene that encodes DNA polymerase theta. According to some studies, POLQ may have the following features.
POLQ inhibits genomic instability and genomic rearrangements derived from DNA double-strand breaks. POLQ is a member of a similar cluster of up-regulated genes that are strongly associated with poor clinical outcomes in breast, ovarian and other types of cancer. Inhibition of POLQ is a compelling combination therapy for radiosensitization. POLQ-deficient mutants displayed hypersensitivity to H2O2-induced oxidative alkali damage, but not to UV light or cisplatin. Overexpression of POLQ leads to poor prognosis in patients with early breast cancer.