Recombinant Human DNA replication complex GINS protein PSF3 (GINS3)

Code CSB-YP880092HU
MSDS
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Source Yeast
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Code CSB-EP880092HU
MSDS
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Source E.coli
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Code CSB-EP880092HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP880092HU
MSDS
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Source Baculovirus
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Code CSB-MP880092HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GINS3
Uniprot No.
Alternative Names
DNA replication complex GINS protein PSF3; GINS complex subunit 3; Gins3; PSF3 homolog; PSF3_HUMAN
Species
Homo sapiens (Human)
Expression Region
1-216
Target Protein Sequence
MSEAYFRVES GALGPEENFL SLDDILMSHE KLPVRTETAM PRLGAFFLER SAGAETDNAV PQGSKLELPL WLAKGLFDNK RRILSVELPK IYQEGWRTVF SADPNVVDLH KMGPHFYGFG SQLLHFDSPE NADISQSLLQ TFIGRFRRIM DSSQNAYNED TSALVARLDE MERGLFQTGQ KGLNDFQCWE KGQASQITAS NLVQNYKKRK FTDMED
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA.
Gene References into Functions
  1. The authors show that GINS is a compact tetramer in solution as observed in crystal structures, but also forms a double-tetrameric population, detectable by electron microscopy. PMID: 28071757
  2. expression in primary lung adenocarcinoma plays an important role in disease progression and is a prognostic indicator PMID: 27005977
  3. Psf3 expression was strongly correlated with tumor progression in non-small-cell lung cancer PMID: 26291987
  4. Two downregulated genes, NDRG4 and GINS3, have been located in a genomic interval associated with cardiac repolarization in published GWASs and zebra fish knockout models PMID: 25520251
  5. PSF3 expression plays an important role in the progression of colorectal cancer and acts as a factor significantly affecting the prognosis of patients. PMID: 25403684
  6. a prognostic biomarker in lung adenocarcinoma PMID: 23127337
  7. These results suggest that PSF3 is a potential biomarker for diagnosis of progression in colon cancer and could PMID: 20059967
  8. expression of mammalian GINS is regulated by 17beta-Estradiol-stimulated estrogen receptor alpha, and PSF3 acts as a gene responsive to transcription factor E2F1 PMID: 17127213
  9. This sensitized zebrafish screen identified 15 novel myocardial repolarization genes. Among these genes is GINS3, the human ortholog of which is a major locus in 2 concurrent human genome-wide association studies of QT interval. PMID: 19652097
  10. This paper describes work done in Saccharomyces cerevisiae. PMID: 12730134
  11. This paper describes work done in Schizosaccharomyces pombe. PMID: 16990792

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Subcellular Location
Nucleus. Chromosome.
Protein Families
GINS3/PSF3 family
Database Links

HGNC: 25851

OMIM: 610610

KEGG: hsa:64785

UniGene: Hs.47125

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