Recombinant Human Death ligand signal enhancer (KIAA0141)

Code CSB-YP613488HU
MSDS
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Source Yeast
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Code CSB-EP613488HU
MSDS
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Source E.coli
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Code CSB-EP613488HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP613488HU
MSDS
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Source Baculovirus
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Code CSB-MP613488HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
KIAA0141
Uniprot No.
Alternative Names
DELE1; DELE; KIAA0141DAP3-binding cell death enhancer 1; Death ligand signal enhancer
Species
Homo sapiens (Human)
Expression Region
102-515
Target Protein Sequence
IHFQASLPA GPQRVEHCSW HSPLDRFFSS PLWHPCSSLR QHILPSPDGP APRHTGLREP RLGQEEASAQ PRNFSHNSLR GARPQDPSEE GPGDFGFLHA SSSIESEAKP AQPQPTGEKE QDKSKTLSLE EAVTSIQQLF QLSVSIAFNF LGTENMKSGD HTAAFSYFQK AAARGYSKAQ YNAGLCHEHG RGTPRDISKA VLYYQLAASQ GHSLAQYRYA RCLLRDPASS WNPERQRAVS LLKQAADSGL REAQAFLGVL FTKEPYLDEQ RAVKYLWLAA NNGDSQSRYH LGICYEKGLG VQRNLGEALR CYQQSAALGN EAAQERLRAL FSMGAAAPGP SDLTVTGLKS FSSPSLCSLN TLLAGTSRLP HASSTGNLGL LCRSGHLGAS LEASSRAIPP HPYPLERSVV RLGFG
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Key activator of the integrated stress response (ISR) following mitochondrial stress. In response to mitochondrial stress, cleaved by the protease OMA1, generating the DAP3-binding cell death enhancer 1 short form (DELE1(S) or S-DELE1), which translocates to the cytosol and activates EIF2AK1/HRI to trigger the ISR. Essential for the induction of death receptor-mediated apoptosis through the regulation of caspase activation.; Protein kinase activator generated by protein cleavage in response to mitochondrial stress, which accumulates in the cytosol and specifically binds to and activates the protein kinase activity of EIF2AK1/HRI. It thereby activates the integrated stress response (ISR): EIF2AK1/HRI activation promotes eIF-2-alpha (EIF2S1) phosphorylation, leading to a decrease in global protein synthesis and the induction of selected genes, including the transcription factor ATF4, the master transcriptional regulator of the ISR.
Gene References into Functions
  1. identifies a novel DAP3-binding protein termed death ligand signal enhancer (DELE); demonstrates the biological significance of DELE for apoptosis signal mediated by death receptors PMID: 20563667
Subcellular Location
[DAP3-binding cell death enhancer 1]: Mitochondrion. Mitochondrion inner membrane.; [DAP3-binding cell death enhancer 1 short form]: Cytoplasm, cytosol.
Tissue Specificity
Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.
Database Links

HGNC: 28969

OMIM: 615741

KEGG: hsa:9812

STRING: 9606.ENSP00000194118

UniGene: Hs.210532

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