Recombinant Human Dehydrogenase/reductase SDR family member 11 (DHRS11)

Code CSB-YP744264HU
MSDS
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Source Yeast
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Code CSB-EP744264HU
MSDS
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Source E.coli
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Code CSB-EP744264HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP744264HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP744264HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DHRS11
Uniprot No.
Alternative Names
DHRS11; SDR24C1; UNQ836/PRO1774Dehydrogenase/reductase SDR family member 11; 17-beta-hydroxysteroid dehydrogenase; 3-beta-hydroxysteroid 3-dehydrogenase; EC 1.1.1.270; Estradiol 17-beta-dehydrogenase; EC 1.1.1.62; Short-chain dehydrogenase/reductase family 24C member 1
Species
Homo sapiens (Human)
Expression Region
31-260
Target Protein Sequence
LVQQGLKVVG CARTVGNIEE LAAECKSAGY PGTLIPYRCD LSNEEDILSM FSAIRSQHSG VDICINNAGL ARPDTLLSGS TSGWKDMFNV NVLALSICTR EAYQSMKERN VDDGHIININ SMSGHRVLPL SVTHFYSATK YAVTALTEGL RQELREAQTH IRATCISPGV VETQFAFKLH DKDPEKAAAT YEQMKCLKPE DVAEAVIYVL STPAHIQIGD IQMRPTEQVT
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the conversion of the 17-keto group of estrone, 4- and 5-androstenes and 5-alpha-androstanes into their 17-beta-hydroxyl metabolites and the conversion of the 3-keto group of 3-, 3,17- and 3,20- diketosteroids into their 3-hydroxyl metabolites. Exhibits reductive 3-beta-hydroxysteroid dehydrogenase activity toward 5-beta-androstanes, 5-beta-pregnanes, 4-pregnenes and bile acids. May also reduce endogenous and exogenous alpha-dicarbonyl compounds and xenobiotic alicyclic ketones.
Gene References into Functions
  1. The recombinant protein (DHRS11) efficiently catalyzed the conversion of the 17-keto group of estrone, 4- and 5-androstenes and 5alpha-androstanes into their 17beta-hydroxyl metabolites with NADPH as a coenzyme. PMID: 26920053
Subcellular Location
Secreted.
Protein Families
Short-chain dehydrogenases/reductases (SDR) family
Tissue Specificity
Isoform 1: Ubiquitously expressed, with highest levels in testis, small intestine, colon, kidney, brain and heart. Isoform 3: Expressed in brain, heart and skeletal muscle.
Database Links

HGNC: 28639

OMIM: 616159

KEGG: hsa:79154

STRING: 9606.ENSP00000251312

UniGene: Hs.462859

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