Recombinant Human Dehydrogenase/reductase SDR family member 2, mitochondrial (DHRS2)

Code CSB-YP615674HU
MSDS
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Source Yeast
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Code CSB-EP615674HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP615674HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP615674HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DHRS2
Uniprot No.
Alternative Names
DHRS2; SDR25C1; Dehydrogenase/reductase SDR family member 2; mitochondrial; EC 1.1.1.-; Dicarbonyl reductase HEP27; Protein D; Short chain dehydrogenase/reductase family 25C member 1
Species
Homo sapiens (Human)
Expression Region
1-300
Target Protein Sequence
MLSAVARGYQGWFHPCARLSVRMSSTGIDRKGVLANRVAVVTGSTSGIGFAIARRLARDGAHVVISSRKQQNVDRAMAKLQGEGLSVAGIVCHVGKAEDREQLVAKALEHCGGVDFLVCSAGVNPLVGSTLGTSEQIWDKILSVNVKSPALLLSQLLPYMENRRGAVILVSSIAAYNPVVALGVYNVSKTALLGLTRTLALELAPKDIRVNCVVPGIIKTDFSKVVRIGFMGMSLSGRTSRNIISCRGLGSQRTVQESCPSCALQMPATSTGRTLRWQATPLGSERSGGGCVAVVPGPGA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Displays NADPH-dependent dicarbonyl reductase activity in vitro with 3,4-Hexanedione, 2,3-Heptanedione and 1-Phenyl-1,2-propanedione as substrates. No reductase activity is displayed in vitro with steroids, retinoids and sugars as substrates. Attenuates MDM2-mediated p53/TP53 degradation, leading to p53/TP53 stabilization and increased transcription activity, resulting in the accumulation of MDM2 and CDKN1A/p21.
Gene References into Functions
  1. DHRS2 and DHRS4 genes are syntenic outparalogues originating from a duplication of the DHRS4 gene that took place before the formation of the mammalian clade PMID: 23036705
  2. A rapid divergent evolution brought the human DHRS2 gene, duplicated form of the DHRS4 one, to code a SDR enzyme having subcellular localization, synthesis regulation and specialized cellular functions very different from those of the human DHRS4 enzyme. PMID: 23036705
  3. Complete genomic organization of DHRS2, including two alternative promoter regions: a hepatocyte-specific promoter and a monocyte-derived dendritic cell-specific promoter PMID: 11944995
  4. c-Myb proto-oncogene has a tumor suppressor role in breast cancer via c-Myb controlled DHRS2 (HEP27) expression. PMID: 20949095
  5. Hep27 is regulated at the transcriptional level by the proto-oncogene c-Myb and is required for c-Myb-induced p53 stabilization. PMID: 20547751
  6. Molecular cloning, sequence and Chr 14 localization of the DHRS2 gene. Nuclear and cytoplasmic localization and normal tissue distribution of the DHRS2-encoded Hep27 protein. PMID: 11997086
  7. The synthesis of the nuclear protein D (Hep27 protein old name) is up-regulated in growth-inhibited HepG2 cells and is inhibited during DNA synthesis. PMID: 1847869
  8. In human endometrial carcinoma cells, the Hep27 protein encoded by DHRS2 is specifically upregulated in mitochondria by the ERM/ETV5 transcription factor and Hep27 has a protective role against apoptosis induced by oxidative stress. PMID: 19443906
  9. Hep27 a cell-cycle regulated protein belongs to the SDR family (short-chain dehydrogenase/reductase family). PMID: 7556196
  10. Hep27 is a NADPH-dependent dicarbonyl reductase enzyme active on xenobiotics. PMID: 16685466

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Subcellular Location
Mitochondrion matrix. Nucleus. Note=A minor fraction of the protein is translocated from the mitochondria to the nucleus, after cleavage of the targeting signal.
Protein Families
Short-chain dehydrogenases/reductases (SDR) family
Tissue Specificity
Widely expressed, with highest levels in liver and kidney, followed by heart, spleen, skeletal muscle and placenta. In hemopoietic cells, expressed in dendritic cells, but not in monocytes, macrophages, granulocytes, nor in B and T lymphocytes.
Database Links

HGNC: 18349

OMIM: 615194

KEGG: hsa:10202

STRING: 9606.ENSP00000344674

UniGene: Hs.272499

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