Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Cell Biology
Alternative Names
DHFR; DHFRP1; Dihydrofolate reductase; DYR; DYR_HUMAN; EC 1.5.1.3
Species
Homo sapiens (Human)
Expression Region
1-187aa
Target Protein Sequence
MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
Tris-based buffer,50% glycerol
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The expression region of this recombinant Human DHFR covers amino acids 1-187. The expected molecular weight for the DHFR protein is calculated to be 25.5 kDa. This DHFR protein is produced using e.coli expression system. The DHFR gene fragment has been modified by fusing the N-terminal 6xHis tag, providing convenience in detecting and purifying the recombinant DHFR protein during the following stages.
Human dihydrofolate reductase (DHFR) is a pivotal enzyme in cellular metabolism, essential for the synthesis of nucleic acids. It catalyzes the conversion of dihydrofolate to tetrahydrofolate, a vital cofactor for DNA and RNA synthesis. DHFR plays a crucial role in cell division and proliferation by ensuring an adequate supply of folate-derived cofactors for nucleotide biosynthesis. Due to its significance in cellular processes, DHFR is a target for antifolate drugs, including methotrexate, commonly used in cancer chemotherapy and autoimmune disease treatment. Understanding DHFR's function is vital for developing therapeutic strategies and elucidating folate metabolism intricacies.