Recombinant Human Docking protein 2 (DOK2)

Code CSB-YP007108HU
MSDS
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Source Yeast
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Code CSB-EP007108HU
MSDS
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Source E.coli
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Code CSB-EP007108HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007108HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP007108HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DOK2
Uniprot No.
Alternative Names
Docking protein 2 56kDa; Docking protein 2; DOK 2; DOK R; DOK2; DOK2_HUMAN; Downstream of tyrosine kinase 2; OTTHUMP00000161710; OTTHUMP00000224923; p56(dok 2); p56(dok-2); p56dok 2; p56DOK; p56dok2
Species
Homo sapiens (Human)
Expression Region
1-412
Target Protein Sequence
MGDGAVKQGF LYLQQQQTFG KKWRRFGASL YGGSDCALAR LELQEGPEKP RRCEAARKVI RLSDCLRVAE AGGEASSPRD TSAFFLETKE RLYLLAAPAA ERGDWVQAIC LLAFPGQRKE LSGPEGKQSR PCMEENELYS SAVTVGPHKE FAVTMRPTEA SERCHLRGSY TLRAGESALE LWGGPEPGTQ LYDWPYRFLR RFGRDKVTFS FEAGRRCVSG EGNFEFETRQ GNEIFLALEE AISAQKNAAP ATPQPQPATI PASLPRPDSP YSRPHDSLPP PSPTTPVPAP RPRGQEGEYA VPFDAVARSL GKNFRGILAV PPQLLADPLY DSIEETLPPR PDHIYDEPEG VAALSLYDSP QEPRGEAWRR QATADRDPAG LQHVQPAGQD FSASGWQPGT EYDNVVLKKG PK
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation.
Gene References into Functions
  1. In this report, the authors demonstrated that the cellular adaptor proteins Dok-2 and Dok-1 are tyrosine phosphorylated upon herpes simplex virus 1 infection. In addition, herpes simplex virus 1 induced the selective degradation of Dok-2. Finally, Dok-2 interacts with herpes simplex virus 1 VP11/12, and that herpes simplex virus 1-induced tyrosine phosphorylation and degradation of Dok-2 require VP11/12. PMID: 28841444
  2. DOK2 and DOK3 expression was significantly reduced in HTLV-1-infected T cells. PMID: 27265473
  3. point mutations in DOK1 and DOK2 genes are detected with low frequency in chronic myelomonocytic leukemia but may have consequences for the function of the DOK2 PTB domain PMID: 25252871
  4. Taken together, these results reveal that Dok1 and Dok2 proteins are involved in an intrinsic negative feedback loop downstream of natural killer-cell-activating receptors in mouse and human. PMID: 24963146
  5. DOK2 as a tumor suppressor in EGFR-mutant lung adenocarcinoma PMID: 24255704
  6. DOK2 is a marker of poor prognosis in patients with gastric cancer after curative resection PMID: 22130622
  7. Data indicate there was no evidence of DOK2 somatic mutation in the leukemias analyzed. PMID: 21329978
  8. data indicate that DOK2 is altered in gastric (GC) and colorectal cancers (CRC) by loss of expressions; data indicate that somatic mutation of DOK2 may be rare in GC, CRC, breast cancer, prostate cancer and liver cancer PMID: 21749457
  9. Dok1 negatively regulates Dok2-mediated CD200R signaling through the recruitment of CrkL. PMID: 21078907
  10. Identification of DOK genes as lung tumor suppressors. PMID: 20139980
  11. identified tyrosine residue 1106 on Tie2 as an Angiopoietin 1-dependent autophosphorylation site that mediates binding and phosphorylation of the downstream-of-kinase-related (Dok-R) docking protein PMID: 12665569
  12. Dok-R and c-Abl interact in both a constitutive and inducible fashion and Dok-R influences the intracellular kinase and biological activity of c-Abl PMID: 12777393
  13. Dok-2 tyrosine phosphorylation was also found to be involved in collagen receptor, glycoprotein VI (GPVI), signaling as well as in outside-in signaling through the major platelet integrin, alpha IIIb beta 3 PMID: 14645010
  14. DOK1 and DOK2 interact with the Tec protein tyrosine kinase. PMID: 14647425
  15. Dok-R acts as an EGFR-recruited scaffolding molecule that processively assembles c-Src and Csk to attenuate signaling from the EGFR. PMID: 15831486
  16. Phosphotyrosine-binding mediated oligomerization of Dok-1 and Dok-2 represents an essential step for Dok phosphorylation and function. PMID: 16177091
  17. The data provide evidence that DOK2 protein has a role in regulating cell proliferation and differentiation and is positive regulators of the MAPK signaling pathway in this context. PMID: 16823827
  18. Dok-2 is a critical element of a linker for activation of T cells (LAT)-dependent negative feedback loop that attenuates early T-cell receptor (TCR) signal. PMID: 17043143
  19. results demonstrate differential modes of regulation of Dok1 and Dok2 in platelets, and raise the possibility that Dok2 plays an important role in integrin outside-in signaling through a physical and functional interaction with integrin alphaIIbbeta3 PMID: 17092301
  20. Dok-1/Dok-2 pleckstrin homology domains bind in vitro to the rare phosphoinositide species, phosphatidylinositol 5-phosphate PMID: 19299694
  21. CD200R inhibits the activation of human myeloid cells through direct recruitment of Dok2 and subsequent activation of RAS p21 protein activator 1. PMID: 19786546

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Protein Families
DOK family, Type A subfamily
Tissue Specificity
Highly expressed in peripheral blood leukocytes, lymph nodes and spleen. Lower expression in thymus, bone marrow and fetal liver.
Database Links

HGNC: 2991

OMIM: 604997

KEGG: hsa:9046

STRING: 9606.ENSP00000276420

UniGene: Hs.71215

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