Recombinant Human Doublesex- and mab-3-related transcription factor 1 (DMRT1)

Code CSB-YP896914HU
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Source Yeast
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Code CSB-EP896914HU
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Source E.coli
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Code CSB-EP896914HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP896914HU
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Source Baculovirus
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Code CSB-MP896914HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DMRT1
Uniprot No.
Alternative Names
CT154; DM domain expressed in testis 1; DM domain expressed in testis protein 1; DM domain gene expressed in testis; DMRT 1; dmrt1; DMRT1_HUMAN; DMT 1; DMT1; Doublesex and mab 3 related transcription factor 1; Doublesex- and mab-3-related transcription factor 1
Species
Homo sapiens (Human)
Expression Region
1-373
Target Protein Sequence
MPNDEAFSKP STPSEAPHAP GVPPQGRAGG FGKASGALVG AASGSSAGGS SRGGGSGSGA SDLGAGSKKS PRLPKCARCR NHGYASPLKG HKRFCMWRDC QCKKCNLIAE RQRVMAAQVA LRRQQAQEEE LGISHPIPLP SAAELLVKRE NNGSNPCLMT ECSGTSQPPP ASVPTTAASE GRMVIQDIPA VTSRGHVENT PDLVSDSTYY SSFYQPSLFP YYNNLYNCPQ YSMALAADSA SGEVGNPLGG SPVKNSLRGL PGPYVPGQTG NQWQMKNMEN RHAMSSQYRM HSYYPPPSYL GQSVPQFFTF EDAPSYPEAR ASVFSPPSSQ DSGLVSLSSS SPISNKSTKA VLECEPASEP SSFTVTPVIE EDE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor that plays a key role in male sex determination and differentiation by controlling testis development and male germ cell proliferation. Plays a central role in spermatogonia by inhibiting meiosis in undifferentiated spermatogonia and promoting mitosis, leading to spermatogonial development and allowing abundant and continuous production of sperm. Acts both as a transcription repressor and activator: prevents meiosis by restricting retinoic acid (RA)-dependent transcription and repressing STRA8 expression and promotes spermatogonial development by activating spermatogonial differentiation genes, such as SOHLH1. Also plays a key role in postnatal sex maintenance by maintaining testis determination and preventing feminization: represses transcription of female promoting genes such as FOXL2 and activates male-specific genes. May act as a tumor suppressor. May also play a minor role in oogenesis.
Gene References into Functions
  1. DMRT1 might influence sex-specific patterns of childhood asthma, and its expression in testis tissue and lung macrophages suggests a potential involvement in hormone or immune cell regulation. PMID: 26906082
  2. The results suggest a role of changes in DMRT1 dosage in non-obstructive azoospermia potentially also through a process of gene misregulation, even though DMRT1 deleterious variants seem to be rare. PMID: 26139570
  3. The role of DMT1 in mitochondrial iron acquisition by immunofluorescence with mitochondrial markers in cells and isolated mitochondria, as well as flow cytometric quantification of DMT1-positive mitochondria from an inducible expression system. PMID: 25483589
  4. These show that DMRT proteins use a unique binding interaction, inserting two adjacent antiparallel recognition helices into a widened DNA major groove to make base-specific contacts. PMID: 26005864
  5. Point mutations of DMRT1 may be rarely associated with male infertility. PMID: 24934491
  6. The combined results indicate that DMRT1 loss-of-function mutations are a risk factor and potential genetic cause of human spermatogenic failure PMID: 23555275
  7. neither DMRT1 nor FGF9 abnormalities are frequently involved in dysgenetic male gonad development in patients with non-syndromic 46,XY disorder of sex development. PMID: 22939835
  8. Meiosis signalling is dysregulated in carcinoma in situ (CIS) cells and that a key regulator of the mitosis-meiosis switch, DMRT1, is expressed in 'early-stage' CIS cells but is down-regulated with further invasive transformation. PMID: 23303528
  9. The biological importance of the changes in expression of DMRT1 in Sertoli cells remains to be established, but it is consistent with DMRT1 reinforcing the inhibition of meiosis in the testis. PMID: 22899867
  10. Findings continue to corroborate that genes influencing male germ cell development and differentiation have emerged as the major players in inherited TGCT susceptibility. PMID: 21551455
  11. variants in or near BAK1, DMRT1, TERT-CLPTM1L, and KITLG predispose to familial and bilateral TGCT. PMID: 21617256
  12. There is evidence that DMT1 expression and function respond rapidly to changes in dietary iron content PMID: 21462105
  13. Chromosome 9p loss is the hallmark of squamous cell carcinoma, and DMRT1, DMRT3 and DOCK8 genes at 9p24.3 might be of interest for the study of the pathophysiology of SCC as potential targets for therapeutic measures. PMID: 20596660
  14. Studies identified three new susceptibility loci DMRT1, TERT and ATF7IP associated with testicular germ cell cancer. PMID: 20543847
  15. Analysis of germ cell tumors reveals gene expression changes correlated to DMRT1 levels. PMID: 20007774
  16. Comparison of genomic and mRNA sequences show that DMRT1b and c result from exonization of intronic sequences, including the exonization of an Alu element. All 99 Alu elements are non-randomly distributed among the non-coding regions on both directions. PMID: 16617334

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Involvement in disease
Testicular germ cell tumor (TGCT); 46,XY sex reversal 4 (SRXY4)
Subcellular Location
Nucleus.
Protein Families
DMRT family
Tissue Specificity
Testis-specific. Expressed in prostate cancer (at protein level).
Database Links

HGNC: 2934

OMIM: 154230

KEGG: hsa:1761

STRING: 9606.ENSP00000371711

UniGene: Hs.98586

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