Recombinant Human Dual specificity protein phosphatase 12 (DUSP12)

Code CSB-YP890767HU
MSDS
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Source Yeast
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Code CSB-EP890767HU
MSDS
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Source E.coli
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Code CSB-EP890767HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP890767HU
MSDS
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Source Baculovirus
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Code CSB-MP890767HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DUSP12
Uniprot No.
Alternative Names
Dual specificity phosphatase 12; Dual specificity protein phosphatase 12; Dual specificity tyrosine phosphatase YVH1; DUS12_HUMAN; DUSP 1; DUSP 12; DUSP1; DUSP12; Serine/threonine specific protein phosphatase; TDSP4; YVH 1; YVH1; YVH1 protein tyrosine phosphatase (S. cerevisiae) ortholog; YVH1 protein tyrosine phosphatase ortholog
Species
Homo sapiens (Human)
Expression Region
1-340
Target Protein Sequence
MLEAPGPSDG CELSNPSASR VSCAGQMLEV QPGLYFGGAA AVAEPDHLRE AGITAVLTVD SEEPSFKAGP GVEDLWRLFV PALDKPETDL LSHLDRCVAF IGQARAEGRA VLVHCHAGVS RSVAIITAFL MKTDQLPFEK AYEKLQILKP EAKMNEGFEW QLKLYQAMGY EVDTSSAIYK QYRLQKVTEK YPELQNLPQE LFAVDPTTVS QGLKDEVLYK CRKCRRSLFR SSSILDHREG SGPIAFAHKR MTPSSMLTTG RQAQCTSYFI EPVQWMESAL LGVMDGQLLC PKCSAKLGSF NWYGEQCSCG RWITPAFQIH KNRVDEMKIL PVLGSQTGKI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Dual specificity phosphatase; can dephosphorylate both phosphotyrosine and phosphoserine or phosphothreonine residues. Can dephosphorylate glucokinase (in vitro). Has phosphatase activity with the synthetic substrate 6,8-difluoro-4-methylumbelliferyl phosphate and other in vitro substrates.
Gene References into Functions
  1. Results from a study on gene variability markers in early-stage human embryos shows that DUSP12 is a putative variability marker for the 3-day, 8-cell embryo stage. PMID: 26288249
  2. A significant decrease in DUSP12 expression in hypertrophic hearts and cardiomyocytes. PMID: 27702885
  3. These results propagate a role for dual specificity phosphatases at RNP particles and suggest that hYVH1 may affect a variety of fundamental cellular processes by regulating messenger ribonucleoprotein (mRNP) dynamics. PMID: 27856639
  4. hYVH1 is a novel modulator of cell cycle progression mediated by a phosphorylation site located at the C-terminal end of the zinc-binding domain PMID: 21521943
  5. propose that the BRC repeats in BRCA2 cooperate in a partially redundant but reinforcing manner to ensure a high probability of RAD51 filament formation PMID: 21556130
  6. these data suggests common SNPs within DUSP12-ATF6 locus may not play a major role in glucose metabolism in the Chinese. PMID: 21211013
  7. tudies confirmed elevated expression of three target antigens RAB38, TBCE, and DUSP12 in CML. PMID: 20103624
  8. Our data suggest that sequences in or upstream of DUSP12 may contribute to type 2 diabetes susceptibility, but the lack of replication suggests a small effect size. PMID: 16936214
  9. hYVH1 is a novel cell survival phosphatase that co-operates with Hsp70 to positively affect cell viability in response to cellular insults. PMID: 18973475
  10. Data determined that hYVH1 forms intramolecular disulfide bonds at the catalytic cleft as well as within the zinc binding domain to avoid irreversible inactivation during severe oxidative stress. PMID: 19567874

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Subcellular Location
Nucleus. Cytoplasm, cytosol.
Protein Families
Protein-tyrosine phosphatase family, Non-receptor class dual specificity subfamily
Tissue Specificity
Ubiquitous, highest expression in spleen, testis, ovary, and peripheral blood leukocytes and lower expression in liver and lung.
Database Links

HGNC: 3067

OMIM: 604835

KEGG: hsa:11266

STRING: 9606.ENSP00000356920

UniGene: Hs.416216

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