Recombinant Human Dual specificity protein phosphatase 3 (DUSP3)

Code CSB-YP007255HU
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Source Yeast
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Code CSB-EP007255HU
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Source E.coli
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Code CSB-EP007255HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007255HU
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Source Baculovirus
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Code CSB-MP007255HU
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Source Mammalian cell
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Product Details

>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
Dual specificity phosphatase 3; Dual specificity protein phosphatase 3; Dual specificity protein phosphatase VHR; DUS3_HUMAN; DUSP3; Serine/threonine specific protein phosphatase; Vaccinia H1-related phosphatase; Vaccinia virus phosphatase VH1 related; VHR
Homo sapiens (Human)
Expression Region
Target Protein Sequence
Protein Length
Full length protein
Tag Info
The following tags are available.
N-terminal His-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Please contact us to get it.

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Target Background

Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2.
Gene References into Functions
  1. Nuclear HSP70 leads to enhancement of vaccinia H1-related phosphatase (VHR) activity via protein-protein interaction rather than its molecular chaperone activity, thereby suppressing excessive ERK activation. Downregulation of either VRK3 or HSP70 rendered cells vulnerable to glutamate-induced apoptosis. PMID: 27941812
  2. Data indicated that regulating non-receptor tyrosine kinase FAK and cell migration is a newly revealed function of VHR/DUSP3. PMID: 28759036
  3. The loss of DUSP3 activity markedly increases gamma radiation-induced DNA strand breaks, suggesting a potential novel role for DUSP3 in DNA repair. PMID: 28389334
  4. In PTP1B and VHR, two new allosteric clusters were identified in each enzyme. PMID: 28625849
  5. In the phosphatase-silenced cells, the normal bipolar spindle structure was restored by chemical inhibition of Erk1/2 and ectopic overexpression of Dusp3. We propose that at M phase Dusp3 keeps Erk1/2 activity in check to facilitate normal mitosis. PMID: 27423135
  6. Data suggest levels of gene expression of both DUSP3 (dual specificity phosphatase 3) and PSME3 (proteasome activator subunit 3) are associated with susceptibility to Staphylococcus aureus infection/sepsis in humans and in mouse disease model. PMID: 24901344
  7. Our results demonstrate that DUSP3 plays a key and nonredundant role as a regulator of innate immune responses PMID: 25876765
  8. VHR can dimerize inside cells, and that VHR catalytic activity is reduced upon dimerization. PMID: 24798147
  9. DUSP3 is a pro-angiogenic atypical dual-specificity phosphatase. PMID: 24886454
  10. DUSP3 interacting partners are nucleolar proteins involved in processes related to DNA repair and senescence. PMID: 24245651
  11. we report the first successful site-specific incorporation of sulfotyrisine into VHR through the use of expanding genetic code PMID: 23918168
  12. Proteins containing the class II motifs are efficient VHR substrates in vitro, suggesting that VHR may act on a novel class of yet unidentified Tyr(P) proteins in vivo. PMID: 23322772
  13. Enteric commensal bacteria induce extracellular signal-regulated kinase pathway signaling via formyl peptide receptor-dependent redox modulation of dual specific phosphatase 3 PMID: 21921027
  14. VHR expression enhances the signaling of ErbB receptors and may be involved in NSCLC pathogenesis. PMID: 21262974
  15. VHR, a Vaccinia virus VH1-related dual-specific protein phosphatase, is phosphorylated at Y138 by ZAP-70. PMID: 12447358
  16. VHR is required for cell-cycle progression as it modulates MAP kinase activation in a cell-cycle phase-dependent manner. PMID: 16604064
  17. deregulated expression of BRCA1-IRIS is likely to reduce dependence on normal physiological growth stimuli PMID: 17278098
  18. Small dual-specificity phosphatase VHR selectively dephosphorylates tyrosine-phosphorylated interferon-alpha- and beta-activated transcription factor STAT5, leading to the subsequent inhibition of STAT5 function. PMID: 17785772
  19. Results highlight the importance of a high intracellular Zn(2+) content and the VHR/ZAP-70/ERK1,2-associated pathways in the modulation of LNCaP prostate cancer cell growth. PMID: 18311544
  20. VHR can be considered as a new marker for cancer progression in cervix carcinoma and potential new target for anticancer therapy PMID: 18505570
  21. VHR has a direct role in the inhibition of JNK-dependent apoptosis in LNCaP cells and may therefore have a role in prostate cancer progression. PMID: 19010898

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Subcellular Location
Protein Families
Protein-tyrosine phosphatase family, Non-receptor class dual specificity subfamily
Database Links

HGNC: 3069

OMIM: 600183

KEGG: hsa:1845

STRING: 9606.ENSP00000226004

UniGene: Hs.181046

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