Recombinant Human E3 ubiquitin-protein ligase ZNRF2 (ZNRF2)

Code CSB-YP847746HU
MSDS
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Source Yeast
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Code CSB-EP847746HU
MSDS
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Source E.coli
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Code CSB-EP847746HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP847746HU
MSDS
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Source Baculovirus
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Code CSB-MP847746HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ZNRF2
Uniprot No.
Alternative Names
E3 ubiquitin-protein ligase ZNRF2; Protein Ells2; RING finger protein 202; RNF202; Zinc/RING finger protein 2; Znrf2; ZNRF2_HUMAN
Species
Homo sapiens (Human)
Expression Region
2-242
Target Protein Sequence
GAKQSGPAA ANGRTRAYSG SDLPSSSSGG ANGTAGGGGG ARAAAAGRFP AQVPSAHQPS ASGGAAAAAA APAAPAAPRS RSLGGAVGSV ASGARAAQSP FSIPNSSSGP YGSQDSVHSS PEDGGGGRDR PVGGSPGGPR LVIGSLPAHL SPHMFGGFKC PVCSKFVSSD EMDLHLVMCL TKPRITYNED VLSKDAGECA ICLEELQQGD TIARLPCLCI YHKGCIDEWF EVNRSCPEHP SD
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
May play a role in the establishment and maintenance of neuronal transmission and plasticity via its ubiquitin ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
Gene References into Functions
  1. Results detected significantly higher levels of ZNRF2 in osteosarcoma cells (OS) and provide evidence that ZNRF2 enhances OS cell growth and improves survival against doxorubicin. PMID: 28416774
  2. These findings uncover ZNRF2 as a component of the amino acid sensing machinery that acts upstream of Rag-GTPases and the V-ATPase to activate mTORC1. PMID: 27244671
  3. ZNRF1 and ZNRF2 are new players in regulation of the ubiquitous Na(+)/K(+)ATPase that is tuned to changing demands in many physiological contexts. PMID: 22797923
  4. identification of ZNRF2; data suggest that ZNRF proteins play a role in the establishment and maintenance of neuronal transmission and plasticity via their ubiquitin ligase activity [ZNRF2] PMID: 14561866
Subcellular Location
Endosome membrane; Peripheral membrane protein. Lysosome membrane; Peripheral membrane protein. Cell junction, synapse, presynaptic cell membrane; Peripheral membrane protein.
Tissue Specificity
Highly expressed in the brain, with higher expression during development than in adult. Expressed also in mammary glands, testis, colon and kidney.
Database Links

HGNC: 22316

OMIM: 612061

KEGG: hsa:223082

STRING: 9606.ENSP00000323879

UniGene: Hs.263912

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