Recombinant Human ETS-related transcription factor Elf-1 (ELF1)

Code CSB-YP007596HU
MSDS
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Source Yeast
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Code CSB-EP007596HU
MSDS
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Source E.coli
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Code CSB-EP007596HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007596HU
MSDS
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Source Baculovirus
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Code CSB-MP007596HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ELF1
Uniprot No.
Alternative Names
E74 like factor 1 ; E74-like factor 1; ELF 1; ELF1; ELF1_HUMAN; Ets domain transcription factor; ETS related transcription factor Elf 1; ETS related transcription factor Elf1; ets-related transcription factor; ETS-related transcription factor Elf-1; mElf 1; p70; Sts1; TRANSCRIPTION FACTOR ELF 1
Species
Homo sapiens (Human)
Expression Region
1-619
Target Protein Sequence
MAAVVQQNDL VFEFASNVME DERQLGDPAI FPAVIVEHVP GADILNSYAG LACVEEPNDM ITESSLDVAE EEIIDDDDDD ITLTVEASCH DGDETIETIE AAEALLNMDS PGPMLDEKRI NNNIFSSPED DMVVAPVTHV SVTLDGIPEV METQQVQEKY ADSPGASSPE QPKRKKGRKT KPPRPDSPAT TPNISVKKKN KDGKGNTIYL WEFLLALLQD KATCPKYIKW TQREKGIFKL VDSKAVSRLW GKHKNKPDMN YETMGRALRY YYQRGILAKV EGQRLVYQFK EMPKDLIYIN DEDPSSSIES SDPSLSSSAT SNRNQTSRSR VSSSPGVKGG ATTVLKPGNS KAAKPKDPVE VAQPSEVLRT VQPTQSPYPT QLFRTVHVVQ PVQAVPEGEA ARTSTMQDET LNSSVQSIRT IQAPTQVPVV VSPRNQQLHT VTLQTVPLTT VIASTDPSAG TGSQKFILQA IPSSQPMTVL KENVMLQSQK AGSPPSIVLG PAQVQQVLTS NVQTICNGTV SVASSPSFSA TAPVVTFSPR SSQLVAHPPG TVITSVIKTQ ETKTLTQEVE KKESEDHLKE NTEKTEQQPQ PYVMVVSSSN GFTSQVAMKQ NELLEPNSF
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor that activates the LYN and BLK promoters. Appears to be required for the T-cell-receptor-mediated trans activation of HIV-2 gene expression. Binds specifically to two purine-rich motifs in the HIV-2 enhancer.
Gene References into Functions
  1. ELF1 binding occurs at both TERT promoter mutations in melanoma in vitro such that increased recruitment of GABP is enabled by the spatial architecture of native and novel ETS motifs in the TERT promoter region. PMID: 26553150
  2. ELF-1 binds an important duplicated GGAA cis-acting element at the OAS1 promoter and in cooperation with RB1 and SP1 recruitment contributes to regulation in response to IFN stimulation. PMID: 26643049
  3. Data suggest that Elf-1 may modulate breast cancer progression to some extent without having an impact on survival of breast cancer patients. PMID: 21811762
  4. Expression of FLI-1, ELF-1, and GABP activated the PF4 promoter in HepG2 cells. PMID: 21931859
  5. The expression of Elf-1 and survivin in non-small cell lung cancer is related to differentiation, lymphatic metastasis, clinical stage, and prognosis, and both are positively correlated with microvessel density. PMID: 20346215
  6. the results indicate that insufficient O-GlcNAc modification prevents Elf-1-mediated transcriptional repression and thereby upregulates Tollip gene expression in intestinal epithelial cells. PMID: 21867680
  7. through a genome-wide association in a Hong Kong Chinese cohort and replication in 2 other Asian populations, identified association of ELF1 with systemic lupus erythematosus PMID: 21044949
  8. The expression levels of both Elf-1 and VEGF were significantly related with tumor differentiation, lymphatic metastasis, clinical stage, and postoperative survival time in non-small cell lung cancer. PMID: 19624906
  9. ETS transcription factor ELF1 is an important positive regulator of MEIS1 expression PMID: 20600580
  10. transcriptional regulation of the LAT gene, including the discovery that the Ets transcription factor Elf-1 may play a central role in its expression PMID: 16464244
  11. These results provide new evidence that Elf-1 is involved in transcriptional activation of hPygo2. PMID: 18314487
  12. Elf-1 in combination with Sp1 and GABP reduced FcRgamma promoter activity. PMID: 18378679
  13. aberrantly increased PP2A in lupus T cells dephosphorylates Elf-1 at Thr-231, resulting in limited expression and binding of the 98 kDa Elf-1 form to the CD3zeta and FcRgamma promoters. PMID: 18714041
  14. Includes a comparison of MEF to ELF1. PMID: 10207087

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Subcellular Location
Nucleus.
Protein Families
ETS family
Tissue Specificity
In fetal tissues, it is highly expressed in heart, lung liver and kidney, and weakly expressed in brain. In adult, it is highly expressed in pancreas, spleen, thymus and peripheral blood leukocytes, expressed at moderate levels in heart, placenta, lung, l
Database Links

HGNC: 3316

OMIM: 189973

KEGG: hsa:1997

STRING: 9606.ENSP00000239882

UniGene: Hs.135646

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