Recombinant Human Endophilin-A2 (SH3GL1)

In Stock
Code CSB-EP859949HUb0
Abbreviation Recombinant Human SH3GL1 protein
MSDS
Size $306
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
Uniprot No.
Research Area
Cell Biology
Alternative Names
CNSA1; EEN; EEN fusion partner of MLL; Endophilin-2; Endophilin-A2; Extra 11 19 leukemia fusion gene; Extra eleven-nineteen leukemia fusion gene protein; Fusion partner of MLL; MGC111371; SH3 containing Grb 2 like 1 protein; SH3 containing protein EEN; SH3 domain GRB2 like 1; SH3 domain protein 2B; SH3 domain-containing GRB2-like protein 1; SH3D2B; SH3G1_HUMAN; Sh3gl1; SH3P8
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
1-368aa
Target Protein Sequence
MSVAGLKKQFYKASQLVSEKVGGAEGTKLDDDFKEMEKKVDVTSKAVTEVLARTIEYLQPNPASRAKLTMLNTVSKIRGQVKNPGYPQSEGLLGECMIRHGKELGGESNFGDALLDAGESMKRLAEVKDSLDIEVKQNFIDPLQNLCEKDLKEIQHHLKKLEGRRLDFDYKKKRQGKIPDEELRQALEKFEESKEVAETSMHNLLETDIEQVSQLSALVDAQLDYHRQAVQILDELAEKLKRRMREASSRPKREYKPKPREPFDLGEPEQSNGGFPCTTAPKIAASSSFRSSDKPIRTPSRSMPPLDQPSCKALYDFEPENDGELGFHEGDVITLTNQIDENWYEGMLDGQSGFFPLSYVEVLVPLPQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
Mol. Weight
45.0 kDa
Protein Length
Full Length
Tag Info
N-terminal 10xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Implicated in endocytosis. May recruit other proteins to membranes with high curvature.
Gene References into Functions
  1. Study observed elevated EA2 gene expression in the subcutaneous compared to that in the visceral human adipose tissue. EA2 gene expression negatively correlated with adiponectin and chemerin in visceral adipose tissue, and positively correlated with TNF-alpha in subcutaneous adipose tissue. EA2 gene expression was significantly downregulated during differentiation of preadipocytes in vitro. PMID: 29113429
  2. Our study provides novel evidence of Endo II function in HER2+ cancer cell motility and trafficking of HER2 that relates to effective treatments with trastuzumab or T-DM1. Thus, differential expression of Endo II may relate to sensitivity or resistance to trastuzumab-based therapies for HER2+ cancers PMID: 28974266
  3. Expression level of SH3GL1 was widely upregulated in human OS tissues, and their overexpression was significantly correlated with more aggressive clinicopathological features. PMID: 28702842
  4. Endophilin-A2 was identified as a novel molecule regulating macrophage-derived foam cell formation by mechanisms attributable to clathrin-mediated endocytosis and beyond clathrin-mediated endocytosis PMID: 27741419
  5. inhibition of SH3GL1 reverses multidrug resistance through declining P-glycoprotein expression via the EGFR/ERK/AP-1 pathway. PMID: 27220321
  6. Endo II expression was highest in triple-negative breast cancers tumors compared with other subtypes, and at the level of gene expression, high Endo II was associated with reduced relapse-free survival in patients with basal-like breast cancers. PMID: 25784716
  7. results establish a novel function of endoA2 in clathrin-independent endocytosis PMID: 25517096
  8. these data suggest that EEN may play a pivotal role in excessive cell proliferation and insufficient cell apoptosis of bone marrow plasma cells in multiple myeloma. PMID: 24704450
  9. MIR218 functions as a tumor suppressor by regulating SH3GL1 expression in medulloblastoma cancer cells. PMID: 23970061
  10. SH3GL1 may be involved in the oncogenic process of gliomas and effectively elicit an autologous antibody response in low-grade gliomas. PMID: 23050879
  11. Two endophilin A2 mutants, E264A and E264R, have different effects on how endophilin interacts with other proteins, such as dynamin or beta1-adrenergic receptors. PMID: 22750032
  12. Idiopathic scoliosis is a multifactorial genetic disease and SH3GL1 may be one of the pathogenic genes for this disease. PMID: 22183150
  13. SH3GL1 is possibly one of the disease associated genes of adolescent idiopathic scoliosis. PMID: 20627007
  14. new mechanism for MLL-EEN-mediated leukemogenesis in which MLL-EEN interferes with the Ras-suppressing activities of EBP through direct interaction. PMID: 14551139
  15. EEN part plays an essential role in leukemogenesis caused by MLL-EEN. PMID: 15077184
  16. Our results suggest that distinct subcellular localization of the endophilin A family members probably underpins their diverse cellular functions and indicates a role for EEN/EA2 in the cell cycle. PMID: 15214844
  17. findings reveal a concomitant activation of endocytosis and ERK signaling by BPGAP1 via the coupling of its proline-rich region, which targets EEN and its functional GAP domain PMID: 15944398

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Involvement in disease
In some cases of acute leukemia, a translocation results in the formation of a KMT2A/MLL1-EEN fusion gene.
Subcellular Location
Cytoplasm. Early endosome membrane; Peripheral membrane protein. Cell projection, podosome.
Protein Families
Endophilin family
Tissue Specificity
Ubiquitous. Higher expression in pancreas, placenta, prostate, testis and uterus.
Database Links

HGNC: 10830

OMIM: 601768

KEGG: hsa:6455

STRING: 9606.ENSP00000269886

UniGene: Hs.490415

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