Recombinant Human Exostosin-like 2 (EXTL2), partial

Code CSB-YP880066HU
MSDS
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Source Yeast
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Code CSB-EP880066HU
MSDS
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Source E.coli
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Code CSB-EP880066HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP880066HU
MSDS
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Source Baculovirus
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Code CSB-MP880066HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
EXTL2
Uniprot No.
Alternative Names
4-N-acetylhexosaminyltransferase EXTL2; Alpha 1 4 N acetylhexosaminyltransferase EXTL2; Alpha 1; Alpha GalNAcT EXTL2; Alpha-1; Alpha-GalNAcT EXTL2; Exostoses (multiple) like 2; Exostoses multiple like 2; Exostosin like 2; Exostosin like glycosyltransferase 2; EXT L2; EXT related protein 2 ; EXT-related protein 2; EXTL 2; EXTL2; EXTL2_HUMAN; EXTR 2; EXTR2; Glucuronyl galactosyl proteoglycan 4 alpha N acetylglucosaminyltransferase; Glucuronyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase; Multiple exostoses like 2; Processed exostosin like 2; Processed exostosin-like 2
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Glycosyltransferase required for the biosynthesis of heparan-sulfate and responsible for the alternating addition of beta-1-4-linked glucuronic acid (GlcA) and alpha-1-4-linked N-acetylglucosamine (GlcNAc) units to nascent heparan sulfate chains.
Gene References into Functions
  1. EXTL2 functions to suppress GAG biosynthesis that is enhanced by a xylose kinase and the EXTL2-dependent mechanism that regulates GAG biosynthesis might be a "quality control system" for proteoglycans PMID: 23395820
  2. Lysosomal glycosaminoglycan levels in mucopolysaccharidosis are reduced by EXTL2 gene silencing. PMID: 19690583
  3. donor binding appears to undergo two distinct steps, beginning with the N-acetyl group expelling water from the donor- enzyme complex into the bulk solvent followed by positioning of the donor into the binding site for interactions with the enzyme PMID: 15831490
Subcellular Location
Endoplasmic reticulum membrane; Single-pass type II membrane protein.; [Processed exostosin-like 2]: Secreted. Note=A soluble form is found in the serum.
Protein Families
Glycosyltransferase 47 family
Tissue Specificity
Ubiquitous.
Database Links

HGNC: 3516

OMIM: 602411

KEGG: hsa:2135

STRING: 9606.ENSP00000359131

UniGene: Hs.357637

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