Recombinant Human F-box only protein 28 (FBXO28)

Code CSB-YP889119HU
MSDS
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Source Yeast
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Code CSB-EP889119HU
MSDS
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Source E.coli
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Code CSB-EP889119HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP889119HU
MSDS
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Source Baculovirus
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Code CSB-MP889119HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
FBXO28
Uniprot No.
Alternative Names
CENP 30; Centromere protein 30; F box only protein 28; F box protein 28; F-box only protein 28; Fbx28 ; FBX28_HUMAN; FBXO28; FLJ10766; KIAA0483; OTTHUMP00000035597
Species
Homo sapiens (Human)
Expression Region
1-368
Target Protein Sequence
MAAAAEERMA EEGGGGQGDG GSSLASGSTQ RQPPPPAPQH PQPGSQALPA PALAPDQLPQ NNTLVALPIV AIENILSFMS YDEISQLRLV CKRMDLVCQR MLNQGFLKVE RYHNLCQKQV KAQLPRRESE RRNHSLARHA DILAAVETRL SLLNMTFMKY VDSNLCCFIP GKVIDEIYRV LRYVNSTRAP QRAHEVLQEL RDISSMAMEY FDEKIVPILK RKLPGSDVSG RLMGSPPVPG PSAALTTMQL FSKQNPSRQE VTKLQQQVKT NGAGVTVLRR EISELRTKVQ EQQKQLQDQD QKLLEQTQII GEQNARLAEL ERKLREVMES AVGNSSGSGQ NEESPRKRKK ATEAIDSLRK SKRLRNRK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation.
Gene References into Functions
  1. It was shown on human primary islets that FBXO28 improves pancreatic beta-cell survival under diabetogenic conditions without affecting insulin secretion. PMID: 29587369
  2. Expression levels of TP53BP2, FBXO28, and FAM53A genes were associated with patient survival specifically in ER-positive, TP53-mutated tumors. PMID: 28179588
  3. Fbxo28 regulates topoisomerase IIalpha decatenation activity and plays an important role in maintaining genomic stability. PMID: 27754753
  4. Refinement of the critical region of 1q41q42 microdeletion syndrome identifies FBXO28 as a candidate causative gene for intellectual disability and seizures. PMID: 24357076
  5. Study identified F-box protein, FBXO28 that controls MYC-dependent transcription by non-proteolytic ubiquitylation. Depletion of FBXO28 or overexpression of an F-box mutant unable to support MYC ubiquitylation results in an impairment of MYC-driven transcription, transformation and tumourigenesis. PMID: 23776131

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Subcellular Location
Chromosome, centromere, kinetochore.
Database Links

HGNC: 29046

OMIM: 609100

KEGG: hsa:23219

STRING: 9606.ENSP00000355827

UniGene: Hs.64691

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