Recombinant Human FH1/FH2 domain-containing protein 3 (FHOD3), partial

Code CSB-YP651981HU
MSDS
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Source Yeast
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Code CSB-EP651981HU
MSDS
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Source E.coli
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Code CSB-EP651981HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP651981HU
MSDS
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Source Baculovirus
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Code CSB-MP651981HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
FHOD3
Uniprot No.
Alternative Names
FLJ22717; Formactin2; FH1/FH2 domain containing protein 3; FH1/FH2 domain-containing protein 3; Fhod3; FHOD3_HUMAN; FHOS2; FLJ22297; Formactin-2; Formin homolog overexpressed in spleen 2; Formin homology 2 domain containing 3; hFHOS2; KIAA1695; mKIAA1695
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Actin-organizing protein that may cause stress fiber formation together with cell elongation. Isoform 4 may play a role in actin filament polymerization in cardiomyocytes.
Gene References into Functions
  1. FHOD3 played a role in glioma linear migration. PMID: 26912794
  2. Suggest alpha5beta1/Arp2/Arp3/FHOD3 pathway reprograms the actin cytoskeleton to promote invasive migration and local invasion in vivo. PMID: 26370503
  3. DCM-associated FHOD3 variant may cause DCM by interfering with actin filament assembly. PMID: 24088304
  4. FHOD variants is with hypertrophic cardiomyopathy. PMID: 23255317
  5. C-terminal phosphorylation by ROCK1 is sufficient for FHOD3 activation as evidenced by an increase in F-actin in HeLa cells. PMID: 23052206
  6. Single nucleotide polymorphism in FHOD3 gene is associated with acute lymphoblastic leukemia. PMID: 19066393
  7. actin dynamics regulated by Fhod3 are critical for sarcomere organization in striated muscle cells. PMID: 19706596

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Subcellular Location
Cytoplasm, cytoskeleton.; [Isoform 4]: Cytoplasm, myofibril, sarcomere, Z line. Note=Threonine phosphorylation in isoform 4-specific sequence TDTDEEEEVE is required for targeting to myofibrils in cardiomyocytes.
Protein Families
Formin homology family
Tissue Specificity
Expressed in the heart, kidney and brain. May be down-regulated in various types of heart diseases, including idiopathic dilated, ventricular dilated, familial dilated and perinatal dilated cardiomyopathies, as well as ischemic heart disease (at protein l
Database Links

HGNC: 26178

OMIM: 609691

KEGG: hsa:80206

STRING: 9606.ENSP00000257209

UniGene: Hs.436636

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