Recombinant Human Fanconi anemia-associated protein of 24 kDa (FAAP24)

Code CSB-YP866280HU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP866280HU
MSDS
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Source E.coli
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Code CSB-EP866280HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP866280HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP866280HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
FAAP24
Uniprot No.
Alternative Names
FAAP24; C19orf40Fanconi anemia core complex-associated protein 24; Fanconi anemia-associated protein of 24 kDa
Species
Homo sapiens (Human)
Expression Region
1-215
Target Protein Sequence
MEKNPPDDTG PVHVPLGHIV ANEKWRGSQL AQEMQGKIKL IFEDGLTPDF YLSNRCCILY VTEADLVAGN GYRKRLVRVR NSNNLKGIVV VEKTRMSEQY FPALQKFTVL DLGMVLLPVA SQMEASCLVI QLVQEQTKEP SKNPLLGKKR ALLLSEPSLL RTVQQIPGVG KVKAPLLLQK FPSIQQLSNA SIGELEQVVG QAVAQQIHAF FTQPR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Plays a role in DNA repair through recruitment of the FA core complex to damaged DNA. Regulates FANCD2 monoubiquitination upon DNA damage. Induces chromosomal instability as well as hypersensitivity to DNA cross-linking agents, when repressed. Targets FANCM/FAAP24 complex to the DNA, preferentially to single strand DNA.
Gene References into Functions
  1. This is the first report of an FAAP24 loss of function mutation found in human patients with EBV-associated lymphoproliferation. PMID: 27473539
  2. These results demonstrate dual roles of FAAP24 in DNA damage response against crosslinking lesions, one through the formation of FANCM/FAAP24 heterodimer and the other via its ssDNA-binding activity required in optimized checkpoint activation. PMID: 23999858
  3. Crystal structure of the FANCM-FAAP24 complex. PMID: 23932590
  4. Results show that the first HhH motif of FAAP24 is a potential binding site for DNA, which plays a critical role in targeting FANCM-FAAP24 to chromatin. PMID: 24003026
  5. FANCM and FAAP24 play multiple, while not fully epistatic, roles in maintaining genomic integrity. PMID: 23333308
  6. FAAP24 targets FANCM to structures that mimic intermediates formed during the replication/repair of damaged DNA. PMID: 17289582
  7. FAAP24 is dispensable for DNA binding and branch migration activity of FANCM. PMID: 18206976

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Subcellular Location
Nucleus.
Database Links

HGNC: 28467

OMIM: 610884

KEGG: hsa:91442

STRING: 9606.ENSP00000466121

UniGene: Hs.579899

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